Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration.
TCTP (Translationally Controlled Tumour Protein) is a multifunctional protein that plays a role in the development, immune system, tumour reversion, and maintenance of stem cells. The mRNA of the Tpt1 gene is over-expressed during liver regeneration. But, the function of the protein in regeneration...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5389791?pdf=render |
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| author | Elaiya Raja Subramanian Nino Gopi Daisy Dinesh Kumar Sudalaimani Kalidas Ramamoorthy Subburathinam Balakrishnan Jackson Durairaj Selvan Christyraj Vaithilingaraja Arumugaswami Sudhakar Sivasubramaniam |
| author_facet | Elaiya Raja Subramanian Nino Gopi Daisy Dinesh Kumar Sudalaimani Kalidas Ramamoorthy Subburathinam Balakrishnan Jackson Durairaj Selvan Christyraj Vaithilingaraja Arumugaswami Sudhakar Sivasubramaniam |
| author_sort | Elaiya Raja Subramanian |
| collection | DOAJ |
| description | TCTP (Translationally Controlled Tumour Protein) is a multifunctional protein that plays a role in the development, immune system, tumour reversion, and maintenance of stem cells. The mRNA of the Tpt1 gene is over-expressed during liver regeneration. But, the function of the protein in regeneration is not known. To study the role of the protein in regeneration, the earthworm Eudrilus eugeniae was chosen. First, the full length cDNA of the Tpt1 gene was sequenced. The size of the cDNA is 504 bp and the protein has 167 amino acids. The highest level of TCTP expression was documented in the worm after three days of regeneration. The protein was found to be expressed specifically in the epithelial layer of the skin. During regeneration, the protein expression was found to be the highest at the tip of blastema. The pharmacological suppression of TCTP using nutlin-3 and TCTP RNAi experiments resulted in the failure of the regeneration process. The suppression of TCTP caused the arrest of proliferation in posterior amputated worms. The severe cell death was documented in the amputated region of nutlin-3 injected worm. The silencing of TCTP has blocked the modification of clitellar segments. The experiments confirm that TCTP has major functions in the upstream signalling of cell proliferation in the early regeneration process in E. eugeniae. |
| first_indexed | 2024-12-21T15:21:19Z |
| format | Article |
| id | doaj.art-a92de2e1539f4660bcc2fe7f172f2648 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T15:21:19Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a92de2e1539f4660bcc2fe7f172f26482022-12-21T18:59:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017531910.1371/journal.pone.0175319Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration.Elaiya Raja SubramanianNino Gopi DaisyDinesh Kumar SudalaimaniKalidas RamamoorthySubburathinam BalakrishnanJackson Durairaj Selvan ChristyrajVaithilingaraja ArumugaswamiSudhakar SivasubramaniamTCTP (Translationally Controlled Tumour Protein) is a multifunctional protein that plays a role in the development, immune system, tumour reversion, and maintenance of stem cells. The mRNA of the Tpt1 gene is over-expressed during liver regeneration. But, the function of the protein in regeneration is not known. To study the role of the protein in regeneration, the earthworm Eudrilus eugeniae was chosen. First, the full length cDNA of the Tpt1 gene was sequenced. The size of the cDNA is 504 bp and the protein has 167 amino acids. The highest level of TCTP expression was documented in the worm after three days of regeneration. The protein was found to be expressed specifically in the epithelial layer of the skin. During regeneration, the protein expression was found to be the highest at the tip of blastema. The pharmacological suppression of TCTP using nutlin-3 and TCTP RNAi experiments resulted in the failure of the regeneration process. The suppression of TCTP caused the arrest of proliferation in posterior amputated worms. The severe cell death was documented in the amputated region of nutlin-3 injected worm. The silencing of TCTP has blocked the modification of clitellar segments. The experiments confirm that TCTP has major functions in the upstream signalling of cell proliferation in the early regeneration process in E. eugeniae.http://europepmc.org/articles/PMC5389791?pdf=render |
| spellingShingle | Elaiya Raja Subramanian Nino Gopi Daisy Dinesh Kumar Sudalaimani Kalidas Ramamoorthy Subburathinam Balakrishnan Jackson Durairaj Selvan Christyraj Vaithilingaraja Arumugaswami Sudhakar Sivasubramaniam Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration. PLoS ONE |
| title | Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration. |
| title_full | Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration. |
| title_fullStr | Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration. |
| title_full_unstemmed | Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration. |
| title_short | Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration. |
| title_sort | function of translationally controlled tumor protein tctp in eudrilus eugeniae regeneration |
| url | http://europepmc.org/articles/PMC5389791?pdf=render |
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