Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitis
<p>Abstract</p> <p>Background</p> <p>Complementary and alternative medicine (CAM) is becoming a popular treatment for modulating diverse immune disorders. <it>Phellinus linteus</it> (<it>P. linteus</it>) as one of the CAMs has been used to modula...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/12/159 |
| _version_ | 1811300273174347776 |
|---|---|
| author | Hwang Ji Kwon Ho-Keun Kim Jung-Eun Rho Jeonghae Im Sin-Hyeog |
| author_facet | Hwang Ji Kwon Ho-Keun Kim Jung-Eun Rho Jeonghae Im Sin-Hyeog |
| author_sort | Hwang Ji |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Complementary and alternative medicine (CAM) is becoming a popular treatment for modulating diverse immune disorders. <it>Phellinus linteus</it> (<it>P. linteus</it>) as one of the CAMs has been used to modulate cancers, inflammation and allergic activities<b>.</b> However, little evidence has been shown about its underlying mechanism of action by which it exerts a beneficial role in dermatological disease <it>in vivo</it>. In this study, we examined the immunomodulatory effects of <it>P. linteus</it> on experimental atopic dermatitis (AD) and elucidated its action mechanism.</p> <p>Methods</p> <p>The immunomodulatory effect of total extract of <it>P. linteus</it> on IgE production by human myeloma U266B1 cells was measured by ELISA. To further identify the effective components, <it>P. linteus</it> was fractionated into methanol soluble, water soluble and boiling water soluble extracts. Each extract was treated to U266B1 cells and primary B cells to compare their inhibitory effects on IgE secretion. To test the <it>in vivo</it> efficacy, experimental atopic dermatitis (AD) was established by alternative treatment of DNCB and house dust mite extract into BALB/c mice. Water soluble extract of <it>P. linteus</it> (WA) or ceramide as a positive control were topically applied to ears of atopic mouse every day for 2 weeks and progression of the disease was estimated by the following criteria: (a) ear thickness, clinical score, (b) serum total IgE, IgG and mite specific IgE level by ELSIA, (c) histological examination of ear tissue by H&E staining and (d) cytokine profile of total ear cells and CD4<sup>+</sup> T cells by real time PCR and ELSIA.</p> <p>Results</p> <p>Treatment of total extracts of <it>P. linteus</it> to U266B1 inhibited IgE secretion. Among the diverse extracts of <it>P. linteus</it>, water soluble extract of <it>P. linteus</it> (WA) significantly reduced the IgE production in primary B cells and B cell line U266B1. Moreover, treatment of WA reduced AD symptoms such as ear swelling, erythema, and dryness and decreased recruitment of lymphocyte into the inflamed site. Interestingly WA treatment significantly reduced IgE level without affecting IgG levels and also down-regulated the levels of pathogenic cytokines (IL-4, IL-13, IL-12 and IFN-γ) and chemokines (CCL17 and CCL22) involved in AD development.</p> <p>Conclusions</p> <p>Our study indicates that protective effect of water soluble extract of <it>P. linteus</it> in atopic dermatitis is mediated by inhibiting IgE production and expression of AD associated pathogenic cytokines as well as chemokines, suggesting the beneficial effect of <it>P. linteus</it> to modulate allergic skin disease.</p> |
| first_indexed | 2024-04-13T06:48:36Z |
| format | Article |
| id | doaj.art-a931e046d928449ba58f5e07fa51e8c8 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-04-13T06:48:36Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-a931e046d928449ba58f5e07fa51e8c82022-12-22T02:57:29ZengBMCBMC Complementary and Alternative Medicine1472-68822012-09-0112115910.1186/1472-6882-12-159Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitisHwang JiKwon Ho-KeunKim Jung-EunRho JeonghaeIm Sin-Hyeog<p>Abstract</p> <p>Background</p> <p>Complementary and alternative medicine (CAM) is becoming a popular treatment for modulating diverse immune disorders. <it>Phellinus linteus</it> (<it>P. linteus</it>) as one of the CAMs has been used to modulate cancers, inflammation and allergic activities<b>.</b> However, little evidence has been shown about its underlying mechanism of action by which it exerts a beneficial role in dermatological disease <it>in vivo</it>. In this study, we examined the immunomodulatory effects of <it>P. linteus</it> on experimental atopic dermatitis (AD) and elucidated its action mechanism.</p> <p>Methods</p> <p>The immunomodulatory effect of total extract of <it>P. linteus</it> on IgE production by human myeloma U266B1 cells was measured by ELISA. To further identify the effective components, <it>P. linteus</it> was fractionated into methanol soluble, water soluble and boiling water soluble extracts. Each extract was treated to U266B1 cells and primary B cells to compare their inhibitory effects on IgE secretion. To test the <it>in vivo</it> efficacy, experimental atopic dermatitis (AD) was established by alternative treatment of DNCB and house dust mite extract into BALB/c mice. Water soluble extract of <it>P. linteus</it> (WA) or ceramide as a positive control were topically applied to ears of atopic mouse every day for 2 weeks and progression of the disease was estimated by the following criteria: (a) ear thickness, clinical score, (b) serum total IgE, IgG and mite specific IgE level by ELSIA, (c) histological examination of ear tissue by H&E staining and (d) cytokine profile of total ear cells and CD4<sup>+</sup> T cells by real time PCR and ELSIA.</p> <p>Results</p> <p>Treatment of total extracts of <it>P. linteus</it> to U266B1 inhibited IgE secretion. Among the diverse extracts of <it>P. linteus</it>, water soluble extract of <it>P. linteus</it> (WA) significantly reduced the IgE production in primary B cells and B cell line U266B1. Moreover, treatment of WA reduced AD symptoms such as ear swelling, erythema, and dryness and decreased recruitment of lymphocyte into the inflamed site. Interestingly WA treatment significantly reduced IgE level without affecting IgG levels and also down-regulated the levels of pathogenic cytokines (IL-4, IL-13, IL-12 and IFN-γ) and chemokines (CCL17 and CCL22) involved in AD development.</p> <p>Conclusions</p> <p>Our study indicates that protective effect of water soluble extract of <it>P. linteus</it> in atopic dermatitis is mediated by inhibiting IgE production and expression of AD associated pathogenic cytokines as well as chemokines, suggesting the beneficial effect of <it>P. linteus</it> to modulate allergic skin disease.</p>http://www.biomedcentral.com/1472-6882/12/159 |
| spellingShingle | Hwang Ji Kwon Ho-Keun Kim Jung-Eun Rho Jeonghae Im Sin-Hyeog Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitis BMC Complementary and Alternative Medicine |
| title | Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitis |
| title_full | Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitis |
| title_fullStr | Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitis |
| title_full_unstemmed | Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitis |
| title_short | Immunomodulatory effect of water soluble extract separated from mycelium of <it>Phellinus linteus</it> on experimental atopic dermatitis |
| title_sort | immunomodulatory effect of water soluble extract separated from mycelium of it phellinus linteus it on experimental atopic dermatitis |
| url | http://www.biomedcentral.com/1472-6882/12/159 |
| work_keys_str_mv | AT hwangji immunomodulatoryeffectofwatersolubleextractseparatedfrommyceliumofitphellinuslinteusitonexperimentalatopicdermatitis AT kwonhokeun immunomodulatoryeffectofwatersolubleextractseparatedfrommyceliumofitphellinuslinteusitonexperimentalatopicdermatitis AT kimjungeun immunomodulatoryeffectofwatersolubleextractseparatedfrommyceliumofitphellinuslinteusitonexperimentalatopicdermatitis AT rhojeonghae immunomodulatoryeffectofwatersolubleextractseparatedfrommyceliumofitphellinuslinteusitonexperimentalatopicdermatitis AT imsinhyeog immunomodulatoryeffectofwatersolubleextractseparatedfrommyceliumofitphellinuslinteusitonexperimentalatopicdermatitis |