Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei
The dorsal raphe nucleus (DR) and median raphe nucleus (MR) contain populations of glutamatergic and GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-o...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/65502 |
| _version_ | 1811227744847003648 |
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| author | Zhengchao Xu Zhao Feng Mengting Zhao Qingtao Sun Lei Deng Xueyan Jia Tao Jiang Pan Luo Wu Chen Ayizuohere Tudi Jing Yuan Xiangning Li Hui Gong Qingming Luo Anan Li |
| author_facet | Zhengchao Xu Zhao Feng Mengting Zhao Qingtao Sun Lei Deng Xueyan Jia Tao Jiang Pan Luo Wu Chen Ayizuohere Tudi Jing Yuan Xiangning Li Hui Gong Qingming Luo Anan Li |
| author_sort | Zhengchao Xu |
| collection | DOAJ |
| description | The dorsal raphe nucleus (DR) and median raphe nucleus (MR) contain populations of glutamatergic and GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-optical sectioning tomography to generate a comprehensive whole-brain atlas of inputs and outputs of glutamatergic and GABAergic neurons in the DR and MR. We found that these neurons received inputs from similar upstream brain regions. The glutamatergic and GABAergic neurons in the same raphe nucleus had divergent projection patterns with differences in critical brain regions. Specifically, MR glutamatergic neurons projected to the lateral habenula through multiple pathways. Correlation and cluster analysis revealed that glutamatergic and GABAergic neurons in the same raphe nucleus received heterogeneous inputs and sent different collateral projections. This connectivity atlas further elucidates the anatomical architecture of the raphe nuclei, which could facilitate better understanding of their behavioral functions. |
| first_indexed | 2024-04-12T09:46:50Z |
| format | Article |
| id | doaj.art-a93515dcd0604a538c51411e832e0ed3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:46:50Z |
| publishDate | 2021-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a93515dcd0604a538c51411e832e0ed32022-12-22T03:37:56ZengeLife Sciences Publications LtdeLife2050-084X2021-11-011010.7554/eLife.65502Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nucleiZhengchao Xu0https://orcid.org/0000-0002-9462-1638Zhao Feng1https://orcid.org/0000-0001-5035-7655Mengting Zhao2https://orcid.org/0000-0003-2037-9129Qingtao Sun3https://orcid.org/0000-0003-2167-7750Lei Deng4https://orcid.org/0000-0003-2774-5611Xueyan Jia5https://orcid.org/0000-0002-1221-6357Tao Jiang6https://orcid.org/0000-0002-4487-299XPan Luo7https://orcid.org/0000-0002-3923-2111Wu Chen8https://orcid.org/0000-0001-5673-4888Ayizuohere Tudi9https://orcid.org/0000-0002-2451-2903Jing Yuan10https://orcid.org/0000-0001-9050-4496Xiangning Li11https://orcid.org/0000-0002-3747-2824Hui Gong12https://orcid.org/0000-0001-5519-6248Qingming Luo13https://orcid.org/0000-0002-6725-9311Anan Li14https://orcid.org/0000-0002-5877-4813Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China; HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, ChinaHUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China; HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China; HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China; HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Science, Shanghai, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China; HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, China; School of Biomedical Engineering, Hainan University, Haikou, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China; HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Science, Shanghai, ChinaThe dorsal raphe nucleus (DR) and median raphe nucleus (MR) contain populations of glutamatergic and GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-optical sectioning tomography to generate a comprehensive whole-brain atlas of inputs and outputs of glutamatergic and GABAergic neurons in the DR and MR. We found that these neurons received inputs from similar upstream brain regions. The glutamatergic and GABAergic neurons in the same raphe nucleus had divergent projection patterns with differences in critical brain regions. Specifically, MR glutamatergic neurons projected to the lateral habenula through multiple pathways. Correlation and cluster analysis revealed that glutamatergic and GABAergic neurons in the same raphe nucleus received heterogeneous inputs and sent different collateral projections. This connectivity atlas further elucidates the anatomical architecture of the raphe nuclei, which could facilitate better understanding of their behavioral functions.https://elifesciences.org/articles/65502glutamatergic neuronGABAergic neuronraphe nucleus |
| spellingShingle | Zhengchao Xu Zhao Feng Mengting Zhao Qingtao Sun Lei Deng Xueyan Jia Tao Jiang Pan Luo Wu Chen Ayizuohere Tudi Jing Yuan Xiangning Li Hui Gong Qingming Luo Anan Li Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei eLife glutamatergic neuron GABAergic neuron raphe nucleus |
| title | Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei |
| title_full | Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei |
| title_fullStr | Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei |
| title_full_unstemmed | Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei |
| title_short | Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei |
| title_sort | whole brain connectivity atlas of glutamatergic and gabaergic neurons in the mouse dorsal and median raphe nuclei |
| topic | glutamatergic neuron GABAergic neuron raphe nucleus |
| url | https://elifesciences.org/articles/65502 |
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