Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay
In developing countries, pesticides such as triazophos (TZP) are widely used in agriculture. However, the misuse TZP poses a threat to human health and the environment due to its high chemical and photochemical stability. In this study, we developed a lateral flow assay to determine TZP in cucumber....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1816919 |
| Summary: | In developing countries, pesticides such as triazophos (TZP) are widely used in agriculture. However, the misuse TZP poses a threat to human health and the environment due to its high chemical and photochemical stability. In this study, we developed a lateral flow assay to determine TZP in cucumber. We produced anti-TZP monoclonal antibody with half inhibitory concentration of 2.013 ng/mL according to ELISA. Under optimum conditions, the lateral flow assay had TZP cut-off values of 100 ng/mL in 0.01 M PBS and 250 ng/mL in cucumber, and the results could be visualised with the naked eye in 5 min. our findings revealed that lateral flow assay is a rapid and sensitive method for detect TZP in cucumber. |
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| ISSN: | 0954-0105 1465-3443 |