Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay
In developing countries, pesticides such as triazophos (TZP) are widely used in agriculture. However, the misuse TZP poses a threat to human health and the environment due to its high chemical and photochemical stability. In this study, we developed a lateral flow assay to determine TZP in cucumber....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1816919 |
| _version_ | 1819203686123962368 |
|---|---|
| author | Wenliang Ge Steven Suryoprabowo Hua Kuang Liqiang Liu Shanshan Song |
| author_facet | Wenliang Ge Steven Suryoprabowo Hua Kuang Liqiang Liu Shanshan Song |
| author_sort | Wenliang Ge |
| collection | DOAJ |
| description | In developing countries, pesticides such as triazophos (TZP) are widely used in agriculture. However, the misuse TZP poses a threat to human health and the environment due to its high chemical and photochemical stability. In this study, we developed a lateral flow assay to determine TZP in cucumber. We produced anti-TZP monoclonal antibody with half inhibitory concentration of 2.013 ng/mL according to ELISA. Under optimum conditions, the lateral flow assay had TZP cut-off values of 100 ng/mL in 0.01 M PBS and 250 ng/mL in cucumber, and the results could be visualised with the naked eye in 5 min. our findings revealed that lateral flow assay is a rapid and sensitive method for detect TZP in cucumber. |
| first_indexed | 2024-12-23T04:23:51Z |
| format | Article |
| id | doaj.art-a93991c155e042849c68fb3d4aa72603 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-23T04:23:51Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-a93991c155e042849c68fb3d4aa726032022-12-21T18:00:11ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-013111051106010.1080/09540105.2020.18169191816919Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assayWenliang Ge0Steven Suryoprabowo1Hua Kuang2Liqiang Liu3Shanshan Song4Wuxi No.2 people’s hospitalState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan UniversityIn developing countries, pesticides such as triazophos (TZP) are widely used in agriculture. However, the misuse TZP poses a threat to human health and the environment due to its high chemical and photochemical stability. In this study, we developed a lateral flow assay to determine TZP in cucumber. We produced anti-TZP monoclonal antibody with half inhibitory concentration of 2.013 ng/mL according to ELISA. Under optimum conditions, the lateral flow assay had TZP cut-off values of 100 ng/mL in 0.01 M PBS and 250 ng/mL in cucumber, and the results could be visualised with the naked eye in 5 min. our findings revealed that lateral flow assay is a rapid and sensitive method for detect TZP in cucumber.http://dx.doi.org/10.1080/09540105.2020.1816919immunochromatographic assaymonoclonal antibodypesticidesstrip testtriazophos |
| spellingShingle | Wenliang Ge Steven Suryoprabowo Hua Kuang Liqiang Liu Shanshan Song Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay Food and Agricultural Immunology immunochromatographic assay monoclonal antibody pesticides strip test triazophos |
| title | Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay |
| title_full | Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay |
| title_fullStr | Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay |
| title_full_unstemmed | Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay |
| title_short | Rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay |
| title_sort | rapid detection of triazophos in cucumber using lateral flow immunochromatographic assay |
| topic | immunochromatographic assay monoclonal antibody pesticides strip test triazophos |
| url | http://dx.doi.org/10.1080/09540105.2020.1816919 |
| work_keys_str_mv | AT wenliangge rapiddetectionoftriazophosincucumberusinglateralflowimmunochromatographicassay AT stevensuryoprabowo rapiddetectionoftriazophosincucumberusinglateralflowimmunochromatographicassay AT huakuang rapiddetectionoftriazophosincucumberusinglateralflowimmunochromatographicassay AT liqiangliu rapiddetectionoftriazophosincucumberusinglateralflowimmunochromatographicassay AT shanshansong rapiddetectionoftriazophosincucumberusinglateralflowimmunochromatographicassay |