Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise
Physical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2020.576150/full |
| _version_ | 1819275460805132288 |
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| author | Alexandra Brahmer Alexandra Brahmer Elmo W. I. Neuberger Perikles Simon Eva-Maria Krämer-Albers |
| author_facet | Alexandra Brahmer Alexandra Brahmer Elmo W. I. Neuberger Perikles Simon Eva-Maria Krämer-Albers |
| author_sort | Alexandra Brahmer |
| collection | DOAJ |
| description | Physical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of cell-cell-communication. Different types of physical exercise interventions trigger the release of diverse EV subpopulations, which are hypothesized to be involved in physiological adaptation processes leading to health benefits and longevity. Large EVs (“microvesicles” and “microparticles”) are studied frequently in the context of physical exercise using straight forward flow cytometry approaches. However, the analysis of small EVs (sEVs) including exosomes is hampered by the complex composition of blood, confounding the methodology of EV isolation and characterization. This mini review presents a concise overview of the current state of research on sEVs released upon physical exercise (ExerVs), highlighting the technical limits of ExerV analysis. The purity of EV preparations is highly influenced by the co-isolation of non-EV structures in the size range or density of EVs, such as lipoproteins and protein aggregates. Technical constraints associated with EV purification challenge the quantification of distinct ExerV populations, the identification of their cargo, and the investigation of their biological functions. Here, we offer recommendations for the isolation and characterization of ExerVs to minimize the effects of these drawbacks. Technological advances in the ExerV research field will improve understanding of the inter-cellular cross-talk induced by physical exercise leading to health benefits. |
| first_indexed | 2024-12-23T23:24:41Z |
| format | Article |
| id | doaj.art-a93ca0e40c06408aa12f878f5035a0a0 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-23T23:24:41Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-a93ca0e40c06408aa12f878f5035a0a02022-12-21T17:26:14ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-12-011110.3389/fphys.2020.576150576150Considerations for the Analysis of Small Extracellular Vesicles in Physical ExerciseAlexandra Brahmer0Alexandra Brahmer1Elmo W. I. Neuberger2Perikles Simon3Eva-Maria Krämer-Albers4Extracellular Vesicles Research Group, Institute of Developmental Biology and Neurobiology, Johannes Gutenberg University of Mainz, Mainz, GermanyDepartment of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University of Mainz, Mainz, GermanyDepartment of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University of Mainz, Mainz, GermanyDepartment of Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg University of Mainz, Mainz, GermanyExtracellular Vesicles Research Group, Institute of Developmental Biology and Neurobiology, Johannes Gutenberg University of Mainz, Mainz, GermanyPhysical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of cell-cell-communication. Different types of physical exercise interventions trigger the release of diverse EV subpopulations, which are hypothesized to be involved in physiological adaptation processes leading to health benefits and longevity. Large EVs (“microvesicles” and “microparticles”) are studied frequently in the context of physical exercise using straight forward flow cytometry approaches. However, the analysis of small EVs (sEVs) including exosomes is hampered by the complex composition of blood, confounding the methodology of EV isolation and characterization. This mini review presents a concise overview of the current state of research on sEVs released upon physical exercise (ExerVs), highlighting the technical limits of ExerV analysis. The purity of EV preparations is highly influenced by the co-isolation of non-EV structures in the size range or density of EVs, such as lipoproteins and protein aggregates. Technical constraints associated with EV purification challenge the quantification of distinct ExerV populations, the identification of their cargo, and the investigation of their biological functions. Here, we offer recommendations for the isolation and characterization of ExerVs to minimize the effects of these drawbacks. Technological advances in the ExerV research field will improve understanding of the inter-cellular cross-talk induced by physical exercise leading to health benefits.https://www.frontiersin.org/articles/10.3389/fphys.2020.576150/fullphysical exerciseextracellular vesiclestissue cross-talkcirculationplasmaEV isolation |
| spellingShingle | Alexandra Brahmer Alexandra Brahmer Elmo W. I. Neuberger Perikles Simon Eva-Maria Krämer-Albers Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise Frontiers in Physiology physical exercise extracellular vesicles tissue cross-talk circulation plasma EV isolation |
| title | Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise |
| title_full | Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise |
| title_fullStr | Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise |
| title_full_unstemmed | Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise |
| title_short | Considerations for the Analysis of Small Extracellular Vesicles in Physical Exercise |
| title_sort | considerations for the analysis of small extracellular vesicles in physical exercise |
| topic | physical exercise extracellular vesicles tissue cross-talk circulation plasma EV isolation |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2020.576150/full |
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