Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells
BackgroundHyperglycemia, a characteristic feature of diabetes, induces glucotoxicity in pancreatic β-cells, resulting in further impairment of insulin secretion and worsening glycemic control. Thus, preservation of insulin secretory capacity is essential for the management of type 2 diabetes. In thi...
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| Format: | Article |
| Language: | English |
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Korean Diabetes Association
2015-02-01
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| Series: | Diabetes & Metabolism Journal |
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| Online Access: | http://e-dmj.org/Synapse/Data/PDFData/2004DMJ/dmj-39-51.pdf |
| _version_ | 1811211576416403456 |
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| author | Hae-Jung Lee Yoon-Jung Choi So-Young Park Jong-Yeon Kim Kyu-Chang Won Jong-Keun Son Yong-Woon Kim |
| author_facet | Hae-Jung Lee Yoon-Jung Choi So-Young Park Jong-Yeon Kim Kyu-Chang Won Jong-Keun Son Yong-Woon Kim |
| author_sort | Hae-Jung Lee |
| collection | DOAJ |
| description | BackgroundHyperglycemia, a characteristic feature of diabetes, induces glucotoxicity in pancreatic β-cells, resulting in further impairment of insulin secretion and worsening glycemic control. Thus, preservation of insulin secretory capacity is essential for the management of type 2 diabetes. In this study, we evaluated the ability of an Orthosiphon stamineus (OS) extract to prevent glucotoxicity in insulin-producing cells.MethodsWe measured insulin mRNA expression and glucose-stimulated insulin secretion (GSIS) in OS-treated INS-1 cells after exposure to a high glucose (HG; 30 mM) concentration.ResultsThe hexane extract of OS elevated mRNA expression of insulin as well as pancreatic and duodenal homeobox-1 of INS-1 cells in a dose-dependent manner. The hexane OS extract also increased the levels of phosphorylated phosphatidylinositol 3-kinase (PI3K) in a concentration-dependent manner. Additionally, Akt phosphorylation was elevated by treatment with 100 and 200 µmol of the hexane OS extract. Three days of HG exposure suppressed insulin mRNA expression and GSIS; these expressions were restored by treatment with the hexane OS extract. HG elevated peroxide levels in the INS-1 cells. These levels were unaffected by OS treatment under both normal and hyperglycemic conditions.ConclusionOur results suggested that the hexane extract of OS elevates insulin mRNA expression and prevents glucotoxicity induced by a 3-day treatment with HG. This was associated with the activation of PI-3K and Akt. |
| first_indexed | 2024-04-12T05:15:05Z |
| format | Article |
| id | doaj.art-a93ddc1b7f584e3aa125e5ce5005f287 |
| institution | Directory Open Access Journal |
| issn | 2233-6079 2233-6087 |
| language | English |
| last_indexed | 2024-04-12T05:15:05Z |
| publishDate | 2015-02-01 |
| publisher | Korean Diabetes Association |
| record_format | Article |
| series | Diabetes & Metabolism Journal |
| spelling | doaj.art-a93ddc1b7f584e3aa125e5ce5005f2872022-12-22T03:46:39ZengKorean Diabetes AssociationDiabetes & Metabolism Journal2233-60792233-60872015-02-01391515810.4093/dmj.2015.39.1.5114730Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 CellsHae-Jung LeeYoon-Jung ChoiSo-Young ParkJong-Yeon KimKyu-Chang WonJong-Keun SonYong-Woon KimBackgroundHyperglycemia, a characteristic feature of diabetes, induces glucotoxicity in pancreatic β-cells, resulting in further impairment of insulin secretion and worsening glycemic control. Thus, preservation of insulin secretory capacity is essential for the management of type 2 diabetes. In this study, we evaluated the ability of an Orthosiphon stamineus (OS) extract to prevent glucotoxicity in insulin-producing cells.MethodsWe measured insulin mRNA expression and glucose-stimulated insulin secretion (GSIS) in OS-treated INS-1 cells after exposure to a high glucose (HG; 30 mM) concentration.ResultsThe hexane extract of OS elevated mRNA expression of insulin as well as pancreatic and duodenal homeobox-1 of INS-1 cells in a dose-dependent manner. The hexane OS extract also increased the levels of phosphorylated phosphatidylinositol 3-kinase (PI3K) in a concentration-dependent manner. Additionally, Akt phosphorylation was elevated by treatment with 100 and 200 µmol of the hexane OS extract. Three days of HG exposure suppressed insulin mRNA expression and GSIS; these expressions were restored by treatment with the hexane OS extract. HG elevated peroxide levels in the INS-1 cells. These levels were unaffected by OS treatment under both normal and hyperglycemic conditions.ConclusionOur results suggested that the hexane extract of OS elevates insulin mRNA expression and prevents glucotoxicity induced by a 3-day treatment with HG. This was associated with the activation of PI-3K and Akt.http://e-dmj.org/Synapse/Data/PDFData/2004DMJ/dmj-39-51.pdfGlucose-stimulated insulin secretion, Insulin mRNAGlucotoxicity |
| spellingShingle | Hae-Jung Lee Yoon-Jung Choi So-Young Park Jong-Yeon Kim Kyu-Chang Won Jong-Keun Son Yong-Woon Kim Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells Diabetes & Metabolism Journal Glucose-stimulated insulin secretion, Insulin mRNA Glucotoxicity |
| title | Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells |
| title_full | Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells |
| title_fullStr | Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells |
| title_full_unstemmed | Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells |
| title_short | Hexane Extract of Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells |
| title_sort | hexane extract of induces insulin expression and prevents glucotoxicity in ins 1 cells |
| topic | Glucose-stimulated insulin secretion, Insulin mRNA Glucotoxicity |
| url | http://e-dmj.org/Synapse/Data/PDFData/2004DMJ/dmj-39-51.pdf |
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