Experimental and theoretical study of the effect of temperature on collagen in aqueous solution
Background and Objectives: An experimental andtheoretical study ofthe effect oftemperature onthe vibrational spectra of an aqueous collagen solution in the range of 60–90°C was carried out. Results: According to the performed experiment, at first at a temperature of 60°C, a...
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| Format: | Article |
| Language: | English |
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Saratov State University
2022-11-01
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| Series: | Известия Саратовского университета. Новая серия Серия: Физика |
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| Online Access: | https://fizika.sgu.ru/sites/fizika.sgu.ru/files/text-pdf/2022/11/fizika_2022_4-55-66.pdf |
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| author | Ten, Galina Nikolaevna Gerasimenko, Aleksandr Yur'evich Savelyev, Mikhail S. Shcherbakova, Natalia Evgen'evna Slepchenkov, Mikhail Mikhailovich Glukhova, Olga Evgen'evna |
| author_facet | Ten, Galina Nikolaevna Gerasimenko, Aleksandr Yur'evich Savelyev, Mikhail S. Shcherbakova, Natalia Evgen'evna Slepchenkov, Mikhail Mikhailovich Glukhova, Olga Evgen'evna |
| author_sort | Ten, Galina Nikolaevna |
| collection | DOAJ |
| description | Background and Objectives: An experimental andtheoretical study ofthe effect oftemperature onthe vibrational spectra of an aqueous collagen solution in the range of 60–90°C was carried out. Results: According to the performed experiment, at first at a temperature of 60°C, an increase in the intensity of the absorption bands of the IR spectra of collagen is observed compared to room temperature, and then, with further heating of the aqueous collagen solution, the intensity of the absorption bands decreases. To explain this effect, a molecular modeling method was used, when the oligopeptide Gly-Pro-Hyp-Gly, the most frequently repeated in the structure of collagen of any type, was selected as the main structural fragment. The interpretation of the vibrational spectra of the selected molecular fragment of collagen showed qualitative and quantitative agreement with the experimental spectra, which made it possible to use it to explain the temperature dependence. Conclusion: An increase in the intensity of the absorption bands of the experimental IR spectrum of collagen at 60°C is associated with a change in the conformation of the zwitter-ion form of the oligopeptide Gly-Pro-Hyp-Gly, which occurs due to the rotation of the bipolar groups N+H3 and CO− relative to each other. A further increase in temperature leads to a decrease in the pH of the medium, and, as a consequence, to the formation of protonated Gly ionic forms located at the ends of the Gly-Pro-Hyp-Gly molecular fragment in an aqueous solution, which is confirmed by the calculation of theoretical spectra and their comparison with experiment. |
| first_indexed | 2024-04-12T05:11:51Z |
| format | Article |
| id | doaj.art-a94429ee2a354b6faa26ac5232c4d864 |
| institution | Directory Open Access Journal |
| issn | 1817-3020 2542-193X |
| language | English |
| last_indexed | 2024-04-12T05:11:51Z |
| publishDate | 2022-11-01 |
| publisher | Saratov State University |
| record_format | Article |
| series | Известия Саратовского университета. Новая серия Серия: Физика |
| spelling | doaj.art-a94429ee2a354b6faa26ac5232c4d8642022-12-22T03:46:44ZengSaratov State UniversityИзвестия Саратовского университета. Новая серия Серия: Физика1817-30202542-193X2022-11-0122433834910.18500/1817-3020-2022-22-4-338-349Experimental and theoretical study of the effect of temperature on collagen in aqueous solutionTen, Galina Nikolaevna0Gerasimenko, Aleksandr Yur'evich1Savelyev, Mikhail S.2Shcherbakova, Natalia Evgen'evna3Slepchenkov, Mikhail Mikhailovich4Glukhova, Olga Evgen'evna5Saratov State University, 410012, Russia, Saratov, Astrakhanskaya street, 83National Research University «Moscow Institute of Electronic Technology», 1, Shokin Sqr., Moscow, Zelenograd, 124498, RussiaNational Research University «Moscow Institute of Electronic Technology», 1, Shokin Sqr., Moscow, Zelenograd, 124498, RussiaRussian Research Anti-Plague Institute «Microbe», Saratov, University street, d. 46, 410005Saratov State University, 410012, Russia, Saratov, Astrakhanskaya street, 83Saratov State University, 410012, Russia, Saratov, Astrakhanskaya street, 83Background and Objectives: An experimental andtheoretical study ofthe effect oftemperature onthe vibrational spectra of an aqueous collagen solution in the range of 60–90°C was carried out. Results: According to the performed experiment, at first at a temperature of 60°C, an increase in the intensity of the absorption bands of the IR spectra of collagen is observed compared to room temperature, and then, with further heating of the aqueous collagen solution, the intensity of the absorption bands decreases. To explain this effect, a molecular modeling method was used, when the oligopeptide Gly-Pro-Hyp-Gly, the most frequently repeated in the structure of collagen of any type, was selected as the main structural fragment. The interpretation of the vibrational spectra of the selected molecular fragment of collagen showed qualitative and quantitative agreement with the experimental spectra, which made it possible to use it to explain the temperature dependence. Conclusion: An increase in the intensity of the absorption bands of the experimental IR spectrum of collagen at 60°C is associated with a change in the conformation of the zwitter-ion form of the oligopeptide Gly-Pro-Hyp-Gly, which occurs due to the rotation of the bipolar groups N+H3 and CO− relative to each other. A further increase in temperature leads to a decrease in the pH of the medium, and, as a consequence, to the formation of protonated Gly ionic forms located at the ends of the Gly-Pro-Hyp-Gly molecular fragment in an aqueous solution, which is confirmed by the calculation of theoretical spectra and their comparison with experiment.https://fizika.sgu.ru/sites/fizika.sgu.ru/files/text-pdf/2022/11/fizika_2022_4-55-66.pdfcollagenmodeling of ir and raman spectraaqueous solutionconformersdependence of vibrational spectra on temperature |
| spellingShingle | Ten, Galina Nikolaevna Gerasimenko, Aleksandr Yur'evich Savelyev, Mikhail S. Shcherbakova, Natalia Evgen'evna Slepchenkov, Mikhail Mikhailovich Glukhova, Olga Evgen'evna Experimental and theoretical study of the effect of temperature on collagen in aqueous solution Известия Саратовского университета. Новая серия Серия: Физика collagen modeling of ir and raman spectra aqueous solution conformers dependence of vibrational spectra on temperature |
| title | Experimental and theoretical study of the effect of temperature on collagen in aqueous solution |
| title_full | Experimental and theoretical study of the effect of temperature on collagen in aqueous solution |
| title_fullStr | Experimental and theoretical study of the effect of temperature on collagen in aqueous solution |
| title_full_unstemmed | Experimental and theoretical study of the effect of temperature on collagen in aqueous solution |
| title_short | Experimental and theoretical study of the effect of temperature on collagen in aqueous solution |
| title_sort | experimental and theoretical study of the effect of temperature on collagen in aqueous solution |
| topic | collagen modeling of ir and raman spectra aqueous solution conformers dependence of vibrational spectra on temperature |
| url | https://fizika.sgu.ru/sites/fizika.sgu.ru/files/text-pdf/2022/11/fizika_2022_4-55-66.pdf |
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