Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved]
Accessory gland proteins (ACPs) are important reproductive proteins produced by the male accessory glands (MAGs) of most insect species. These proteins are essential for male insect fertility, and are transferred alongside semen to females during copulation. ACPs are poorly characterized in Glossina...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wellcome
2017-11-01
|
| Series: | Wellcome Open Research |
| Subjects: | |
| Online Access: | https://wellcomeopenresearch.org/articles/2-73/v2 |
| _version_ | 1828464986361430016 |
|---|---|
| author | Muna F. Abry Kelvin M. Kimenyi Daniel Masiga Benard W. Kulohoma |
| author_facet | Muna F. Abry Kelvin M. Kimenyi Daniel Masiga Benard W. Kulohoma |
| author_sort | Muna F. Abry |
| collection | DOAJ |
| description | Accessory gland proteins (ACPs) are important reproductive proteins produced by the male accessory glands (MAGs) of most insect species. These proteins are essential for male insect fertility, and are transferred alongside semen to females during copulation. ACPs are poorly characterized in Glossina species (tsetse fly), the principal vector of the parasite that causes life-threatening Human African Trypanosomiasis and Animal trypanosomiasis in endemic regions in Africa. The tsetse fly has a peculiar reproductive cycle because of the absence of oviposition. Females mate once and store sperm in a spermathecal, and produce a single fully developed larva at a time that pupates within minutes of exiting their uterus. This slow reproductive cycle, compared to other insects, significantly restricts reproduction to only 3 to 6 larvae per female lifespan. This unique reproductive cycle is an attractive vector control strategy entry point. We exploit comparative genomics approaches to explore the diversity of ACPs in the recently available whole genome sequence data from five tsetse fly species ( Glossina morsitans, G. austeni, G. brevipalpis, G. pallidipes and G. fuscipes). We used previously described ACPs in Drosophila melanogaster and Anopheles gambiae as reference sequences. We identified 36, 27, 31, 29 and 33 diverse ACP orthologous genes in G. austeni, G. brevipalpis, G. fuscipes, G. pallidipes and G. morsitans genomes respectively, which we classified into 21 functional classes. Our findings provide genetic evidence of MAG proteins in five recently sequenced Glossina genomes. It highlights new avenues for molecular studies that evaluate potential field control strategies of these important vectors of human and animal disease. |
| first_indexed | 2024-12-11T03:24:12Z |
| format | Article |
| id | doaj.art-a9458ababb8f4eb89b698f5ae57f3b0a |
| institution | Directory Open Access Journal |
| issn | 2398-502X |
| language | English |
| last_indexed | 2024-12-11T03:24:12Z |
| publishDate | 2017-11-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj.art-a9458ababb8f4eb89b698f5ae57f3b0a2022-12-22T01:22:33ZengWellcomeWellcome Open Research2398-502X2017-11-01210.12688/wellcomeopenres.12445.214370Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved]Muna F. Abry0Kelvin M. Kimenyi1Daniel Masiga2Benard W. Kulohoma3Center for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, KenyaCenter for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, KenyaInternational Centre for Insect Physiology and Ecology, P.O. Box 30772, Nairobi, 00100, KenyaCenter for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, KenyaAccessory gland proteins (ACPs) are important reproductive proteins produced by the male accessory glands (MAGs) of most insect species. These proteins are essential for male insect fertility, and are transferred alongside semen to females during copulation. ACPs are poorly characterized in Glossina species (tsetse fly), the principal vector of the parasite that causes life-threatening Human African Trypanosomiasis and Animal trypanosomiasis in endemic regions in Africa. The tsetse fly has a peculiar reproductive cycle because of the absence of oviposition. Females mate once and store sperm in a spermathecal, and produce a single fully developed larva at a time that pupates within minutes of exiting their uterus. This slow reproductive cycle, compared to other insects, significantly restricts reproduction to only 3 to 6 larvae per female lifespan. This unique reproductive cycle is an attractive vector control strategy entry point. We exploit comparative genomics approaches to explore the diversity of ACPs in the recently available whole genome sequence data from five tsetse fly species ( Glossina morsitans, G. austeni, G. brevipalpis, G. pallidipes and G. fuscipes). We used previously described ACPs in Drosophila melanogaster and Anopheles gambiae as reference sequences. We identified 36, 27, 31, 29 and 33 diverse ACP orthologous genes in G. austeni, G. brevipalpis, G. fuscipes, G. pallidipes and G. morsitans genomes respectively, which we classified into 21 functional classes. Our findings provide genetic evidence of MAG proteins in five recently sequenced Glossina genomes. It highlights new avenues for molecular studies that evaluate potential field control strategies of these important vectors of human and animal disease.https://wellcomeopenresearch.org/articles/2-73/v2GenomicsParasitology |
| spellingShingle | Muna F. Abry Kelvin M. Kimenyi Daniel Masiga Benard W. Kulohoma Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved] Wellcome Open Research Genomics Parasitology |
| title | Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved] |
| title_full | Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved] |
| title_fullStr | Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved] |
| title_full_unstemmed | Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved] |
| title_short | Comparative genomics identifies male accessory gland proteins in five Glossina species [version 2; referees: 2 approved] |
| title_sort | comparative genomics identifies male accessory gland proteins in five glossina species version 2 referees 2 approved |
| topic | Genomics Parasitology |
| url | https://wellcomeopenresearch.org/articles/2-73/v2 |
| work_keys_str_mv | AT munafabry comparativegenomicsidentifiesmaleaccessoryglandproteinsinfiveglossinaspeciesversion2referees2approved AT kelvinmkimenyi comparativegenomicsidentifiesmaleaccessoryglandproteinsinfiveglossinaspeciesversion2referees2approved AT danielmasiga comparativegenomicsidentifiesmaleaccessoryglandproteinsinfiveglossinaspeciesversion2referees2approved AT benardwkulohoma comparativegenomicsidentifiesmaleaccessoryglandproteinsinfiveglossinaspeciesversion2referees2approved |