Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>)
Ensuring the humane harvest of farmed fish without compromising the quality of the fresh product is paramount to the welfare of fish and in meeting consumer demands. Electrical stunning is a quick and effective way to render fish unconscious and it has emerged as the suggested harvest method by EFSA...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Aquaculture Journal |
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| Online Access: | https://www.mdpi.com/2673-9496/2/4/18 |
| _version_ | 1797615671658414080 |
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| author | Rafael Angelakopoulos Arkadios Dimitroglou Leonidas Papaharisis Katerina A. Moutou |
| author_facet | Rafael Angelakopoulos Arkadios Dimitroglou Leonidas Papaharisis Katerina A. Moutou |
| author_sort | Rafael Angelakopoulos |
| collection | DOAJ |
| description | Ensuring the humane harvest of farmed fish without compromising the quality of the fresh product is paramount to the welfare of fish and in meeting consumer demands. Electrical stunning is a quick and effective way to render fish unconscious and it has emerged as the suggested harvest method by EFSA and OIE. The present study evaluated the effects of electrical stunning on the biochemical processes that lead to fillet degradation <i>postmortem</i>, in the red seabream (<i>Pagrus major</i>). Two distinct electrical stunning conditions (low and high) were compared along with the conventional harvest method (an ice slurry). The activity patterns of calpain, collagenase, and cathepsin B and L were assessed and compared to stereological changes in white muscles at different time points up to 13 days post-harvest. Histological examinations, independent of the harvest technique, revealed a progressively declining trend in fiber volume density and increasing interfibrillar spaces over time, indicative of degradation activity within and between the muscle fibers. Strong correlations between the stereological measures and the individual protease activities were recorded. The higher current condition (electric field 1.8 V/cm and velocity 1.6 m/s) consistently exhibited the lowest protease activity levels and the slowest pace of stereological changes, making it the suggested method of all harvest methods explored. |
| first_indexed | 2024-03-11T07:30:00Z |
| format | Article |
| id | doaj.art-a9460cbb3fff44849fe9e80bc87a63b4 |
| institution | Directory Open Access Journal |
| issn | 2673-9496 |
| language | English |
| last_indexed | 2024-03-11T07:30:00Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Aquaculture Journal |
| spelling | doaj.art-a9460cbb3fff44849fe9e80bc87a63b42023-11-17T07:23:01ZengMDPI AGAquaculture Journal2673-94962022-11-012430231510.3390/aquacj2040018Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>)Rafael Angelakopoulos0Arkadios Dimitroglou1Leonidas Papaharisis2Katerina A. Moutou3Laboratory of Genetics, Comparative and Evolutionary Biology, Department of Biochemistry and Biotechnology, School of Medical Sciences, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, GreeceDepartment of Research and Development, Avramar S.A., 34100 Chalkida, GreeceDepartment of Research and Development, Avramar S.A., 34100 Chalkida, GreeceLaboratory of Genetics, Comparative and Evolutionary Biology, Department of Biochemistry and Biotechnology, School of Medical Sciences, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, GreeceEnsuring the humane harvest of farmed fish without compromising the quality of the fresh product is paramount to the welfare of fish and in meeting consumer demands. Electrical stunning is a quick and effective way to render fish unconscious and it has emerged as the suggested harvest method by EFSA and OIE. The present study evaluated the effects of electrical stunning on the biochemical processes that lead to fillet degradation <i>postmortem</i>, in the red seabream (<i>Pagrus major</i>). Two distinct electrical stunning conditions (low and high) were compared along with the conventional harvest method (an ice slurry). The activity patterns of calpain, collagenase, and cathepsin B and L were assessed and compared to stereological changes in white muscles at different time points up to 13 days post-harvest. Histological examinations, independent of the harvest technique, revealed a progressively declining trend in fiber volume density and increasing interfibrillar spaces over time, indicative of degradation activity within and between the muscle fibers. Strong correlations between the stereological measures and the individual protease activities were recorded. The higher current condition (electric field 1.8 V/cm and velocity 1.6 m/s) consistently exhibited the lowest protease activity levels and the slowest pace of stereological changes, making it the suggested method of all harvest methods explored.https://www.mdpi.com/2673-9496/2/4/18red seabreamelectrical stunningfish harvestflesh qualityproteolytic enzymes |
| spellingShingle | Rafael Angelakopoulos Arkadios Dimitroglou Leonidas Papaharisis Katerina A. Moutou Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>) Aquaculture Journal red seabream electrical stunning fish harvest flesh quality proteolytic enzymes |
| title | Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>) |
| title_full | Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>) |
| title_fullStr | Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>) |
| title_full_unstemmed | Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>) |
| title_short | Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (<i>Pagrus major</i>) |
| title_sort | electrical stunning has the potential to delay fillet degradation post harvest in red seabream i pagrus major i |
| topic | red seabream electrical stunning fish harvest flesh quality proteolytic enzymes |
| url | https://www.mdpi.com/2673-9496/2/4/18 |
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