A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

Objective: To determine an algorithm for molecular diagnosis of visceral leishmaniasis (VL) by kinetoplast DNA (kDNA) (RV1/ RV2) and internal transcriber spacer (ITS1) (LITSR/L5.8S) polymerase chain reaction (PCR), complemented by ITS1 PCR restriction fragment length polymorphism (RFLP), using perip...

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Bibliographic Details
Main Authors:	Natalia Souza de Godoy, Manoel Sebastião da Costa Lima-Junior, José Angelo Lauletta Lindoso, Vera Lucia Pereira-Chioccola, Thelma Suely Okay, Lucia Maria Almeida Braz
Format:	Article
Language:	English
Published:	Wolters Kluwer Medknow Publications 2020-01-01
Series:	Asian Pacific Journal of Tropical Medicine
Subjects:	leishmania infantum; molecular diagnosis; visceral leishmaniasis; pcr; rflp
Online Access:	http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=2;spage=62;epage=70;aulast=de

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