QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
Abstract Background Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient mole...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1537-5 |
| _version_ | 1811211488277299200 |
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| author | L. Pereira V. Ruggieri S. Pérez K. G. Alexiou M. Fernández T. Jahrmann M. Pujol J. Garcia-Mas |
| author_facet | L. Pereira V. Ruggieri S. Pérez K. G. Alexiou M. Fernández T. Jahrmann M. Pujol J. Garcia-Mas |
| author_sort | L. Pereira |
| collection | DOAJ |
| description | Abstract Background Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular breeding tools. Results In order to identify QTLs controlling fruit quality, a recombinant inbred line population was developed using two commercial cultivars as parental lines: “Védrantais”, from the cantalupensis group, and “Piel de Sapo”, from the inodorus group. Both have desirable quality traits for the market, but their fruits differ in traits such as rind and flesh color, sugar content, ripening behavior, size and shape. We used a genotyping-by-sequencing strategy to construct a dense genetic map, which included around five thousand variants distributed in 824 bins. The RIL population was phenotyped for quality and morphology traits, and we mapped 33 stable QTLs involved in sugar and carotenoid content, fruit and seed morphology and major loci controlling external color of immature fruit and mottled rind. The median confidence interval of the QTLs was 942 kb, suggesting that the high density of the genetic map helped in increasing the mapping resolution. Some of these intervals contained less than a hundred annotated genes, and an integrative strategy combining gene expression and resequencing data enabled identification of candidate genes for some of these traits. Conclusion Several QTLs controlling fruit quality traits in melon were identified and delimited to narrow genomic intervals, using a RIL population and a GBS-based genetic map. |
| first_indexed | 2024-04-12T05:14:01Z |
| format | Article |
| id | doaj.art-a946a7d1786d4ed897d7d4ab7fcdc7af |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T05:14:01Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-a946a7d1786d4ed897d7d4ab7fcdc7af2022-12-22T03:46:41ZengBMCBMC Plant Biology1471-22292018-12-0118111710.1186/s12870-018-1537-5QTL mapping of melon fruit quality traits using a high-density GBS-based genetic mapL. Pereira0V. Ruggieri1S. Pérez2K. G. Alexiou3M. Fernández4T. Jahrmann5M. Pujol6J. Garcia-Mas7Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UBCentre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UBCentre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UBCentre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UBSemillas Fitó S.A.Semillas Fitó S.A.Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UBCentre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UBAbstract Background Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular breeding tools. Results In order to identify QTLs controlling fruit quality, a recombinant inbred line population was developed using two commercial cultivars as parental lines: “Védrantais”, from the cantalupensis group, and “Piel de Sapo”, from the inodorus group. Both have desirable quality traits for the market, but their fruits differ in traits such as rind and flesh color, sugar content, ripening behavior, size and shape. We used a genotyping-by-sequencing strategy to construct a dense genetic map, which included around five thousand variants distributed in 824 bins. The RIL population was phenotyped for quality and morphology traits, and we mapped 33 stable QTLs involved in sugar and carotenoid content, fruit and seed morphology and major loci controlling external color of immature fruit and mottled rind. The median confidence interval of the QTLs was 942 kb, suggesting that the high density of the genetic map helped in increasing the mapping resolution. Some of these intervals contained less than a hundred annotated genes, and an integrative strategy combining gene expression and resequencing data enabled identification of candidate genes for some of these traits. Conclusion Several QTLs controlling fruit quality traits in melon were identified and delimited to narrow genomic intervals, using a RIL population and a GBS-based genetic map.http://link.springer.com/article/10.1186/s12870-018-1537-5Quantitative trait lociMelonFruit qualityFruit morphologyGenotyping-by-sequencingGenetic map |
| spellingShingle | L. Pereira V. Ruggieri S. Pérez K. G. Alexiou M. Fernández T. Jahrmann M. Pujol J. Garcia-Mas QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map BMC Plant Biology Quantitative trait loci Melon Fruit quality Fruit morphology Genotyping-by-sequencing Genetic map |
| title | QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map |
| title_full | QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map |
| title_fullStr | QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map |
| title_full_unstemmed | QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map |
| title_short | QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map |
| title_sort | qtl mapping of melon fruit quality traits using a high density gbs based genetic map |
| topic | Quantitative trait loci Melon Fruit quality Fruit morphology Genotyping-by-sequencing Genetic map |
| url | http://link.springer.com/article/10.1186/s12870-018-1537-5 |
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