Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
Background: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment. Methods: Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selec...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/11/5/582 |
| _version_ | 1827670828864503808 |
|---|---|
| author | Yuliya Zboromyrska Verónica Rico Cristina Pitart Mariana José Fernández-Pittol Álex Soriano Jordi Bosch |
| author_facet | Yuliya Zboromyrska Verónica Rico Cristina Pitart Mariana José Fernández-Pittol Álex Soriano Jordi Bosch |
| author_sort | Yuliya Zboromyrska |
| collection | DOAJ |
| description | Background: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment. Methods: Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed. Results: The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases. Conclusion: the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool. |
| first_indexed | 2024-03-10T03:28:32Z |
| format | Article |
| id | doaj.art-a94bbaa69f794c1097ef86db32952922 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:28:32Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a94bbaa69f794c1097ef86db329529222023-11-23T09:48:07ZengMDPI AGAntibiotics2079-63822022-04-0111558210.3390/antibiotics11050582Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological DiagnosisYuliya Zboromyrska0Verónica Rico1Cristina Pitart2Mariana José Fernández-Pittol3Álex Soriano4Jordi Bosch5Microbiology Department, Hospital Clinic, 08036 Barcelona, SpainInfectious Diseases Department, Hospital Clinic, 08036 Barcelona, SpainMicrobiology Department, Hospital Clinic, 08036 Barcelona, SpainMicrobiology Department, Hospital Clinic, 08036 Barcelona, SpainInfectious Diseases Department, Hospital Clinic, 08036 Barcelona, SpainMicrobiology Department, Hospital Clinic, 08036 Barcelona, SpainBackground: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment. Methods: Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed. Results: The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases. Conclusion: the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.https://www.mdpi.com/2079-6382/11/5/582urineMALDI-TOFflow cytometryurinary tract infectionESBLcarbapenemases |
| spellingShingle | Yuliya Zboromyrska Verónica Rico Cristina Pitart Mariana José Fernández-Pittol Álex Soriano Jordi Bosch Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis Antibiotics urine MALDI-TOF flow cytometry urinary tract infection ESBL carbapenemases |
| title | Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis |
| title_full | Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis |
| title_fullStr | Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis |
| title_full_unstemmed | Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis |
| title_short | Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis |
| title_sort | implementation of a new protocol for direct identification from urine in the routine microbiological diagnosis |
| topic | urine MALDI-TOF flow cytometry urinary tract infection ESBL carbapenemases |
| url | https://www.mdpi.com/2079-6382/11/5/582 |
| work_keys_str_mv | AT yuliyazboromyrska implementationofanewprotocolfordirectidentificationfromurineintheroutinemicrobiologicaldiagnosis AT veronicarico implementationofanewprotocolfordirectidentificationfromurineintheroutinemicrobiologicaldiagnosis AT cristinapitart implementationofanewprotocolfordirectidentificationfromurineintheroutinemicrobiologicaldiagnosis AT marianajosefernandezpittol implementationofanewprotocolfordirectidentificationfromurineintheroutinemicrobiologicaldiagnosis AT alexsoriano implementationofanewprotocolfordirectidentificationfromurineintheroutinemicrobiologicaldiagnosis AT jordibosch implementationofanewprotocolfordirectidentificationfromurineintheroutinemicrobiologicaldiagnosis |