Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms
The main purpose of this study was to degrade total petroleum hydrocarbons (TPHs) from contaminated soil in batch microcosm reactors. Native soil fungi isolated from the same petroleum-polluted soil and ligninolytic fungal strains were screened and applied in the treatment of soil-contaminated micro...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1351 |
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| author | Dalel Daâssi Fatimah Qabil Almaghrabi |
| author_facet | Dalel Daâssi Fatimah Qabil Almaghrabi |
| author_sort | Dalel Daâssi |
| collection | DOAJ |
| description | The main purpose of this study was to degrade total petroleum hydrocarbons (TPHs) from contaminated soil in batch microcosm reactors. Native soil fungi isolated from the same petroleum-polluted soil and ligninolytic fungal strains were screened and applied in the treatment of soil-contaminated microcosms in aerobic conditions. The bioaugmentation processes were carried out using selected hydrocarbonoclastic fungal strains in mono or co-cultures. Results demonstrated the petroleum-degrading potential of six fungal isolates, namely KBR1 and KBR8 (indigenous) and KBR1-1, KB4, KB2 and LB3 (exogenous). Based on the molecular and phylogenetic analysis, KBR1 and KB8 were identified as <i>Aspergillus niger</i> [MW699896] and <i>tubingensis</i> [MW699895], while KBR1-1, KB4, KB2 and LB3 were affiliated with the genera <i>Syncephalastrum</i> sp. [MZ817958], <i>Paecilomyces formosus</i> [MW699897], <i>Fusarium chlamydosporum</i> [MZ817957] and <i>Coniochaeta</i> sp. [MW699893], respectively. The highest rate of TPH degradation was recorded in soil microcosm treatments (SMT) after 60 days by inoculation with <i>Paecilomyces formosus</i> 97 ± 2.54%, followed by bioaugmentation with the native strain <i>Aspergillus niger</i> (92 ± 1.83%) and then by the fungal consortium (84 ± 2.21%). The statistical analysis of the results showed significant differences. |
| first_indexed | 2024-03-11T03:28:39Z |
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| id | doaj.art-a95213feab174260a77fa2b8e2aa0c63 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:28:39Z |
| publishDate | 2023-05-01 |
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| series | Microorganisms |
| spelling | doaj.art-a95213feab174260a77fa2b8e2aa0c632023-11-18T02:35:31ZengMDPI AGMicroorganisms2076-26072023-05-01115135110.3390/microorganisms11051351Petroleum-Degrading Fungal Isolates for the Treatment of Soil MicrocosmsDalel Daâssi0Fatimah Qabil Almaghrabi1Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax 3018, TunisiaDepartment of Biology, College of Sciences and Arts, Khulais, University of Jeddah, P.O. Box 34, Jeddah 21959, Saudi ArabiaThe main purpose of this study was to degrade total petroleum hydrocarbons (TPHs) from contaminated soil in batch microcosm reactors. Native soil fungi isolated from the same petroleum-polluted soil and ligninolytic fungal strains were screened and applied in the treatment of soil-contaminated microcosms in aerobic conditions. The bioaugmentation processes were carried out using selected hydrocarbonoclastic fungal strains in mono or co-cultures. Results demonstrated the petroleum-degrading potential of six fungal isolates, namely KBR1 and KBR8 (indigenous) and KBR1-1, KB4, KB2 and LB3 (exogenous). Based on the molecular and phylogenetic analysis, KBR1 and KB8 were identified as <i>Aspergillus niger</i> [MW699896] and <i>tubingensis</i> [MW699895], while KBR1-1, KB4, KB2 and LB3 were affiliated with the genera <i>Syncephalastrum</i> sp. [MZ817958], <i>Paecilomyces formosus</i> [MW699897], <i>Fusarium chlamydosporum</i> [MZ817957] and <i>Coniochaeta</i> sp. [MW699893], respectively. The highest rate of TPH degradation was recorded in soil microcosm treatments (SMT) after 60 days by inoculation with <i>Paecilomyces formosus</i> 97 ± 2.54%, followed by bioaugmentation with the native strain <i>Aspergillus niger</i> (92 ± 1.83%) and then by the fungal consortium (84 ± 2.21%). The statistical analysis of the results showed significant differences.https://www.mdpi.com/2076-2607/11/5/1351single culturefungal consortiumbioaugmentationGC-MSPAH-contaminated soil |
| spellingShingle | Dalel Daâssi Fatimah Qabil Almaghrabi Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms Microorganisms single culture fungal consortium bioaugmentation GC-MS PAH-contaminated soil |
| title | Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms |
| title_full | Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms |
| title_fullStr | Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms |
| title_full_unstemmed | Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms |
| title_short | Petroleum-Degrading Fungal Isolates for the Treatment of Soil Microcosms |
| title_sort | petroleum degrading fungal isolates for the treatment of soil microcosms |
| topic | single culture fungal consortium bioaugmentation GC-MS PAH-contaminated soil |
| url | https://www.mdpi.com/2076-2607/11/5/1351 |
| work_keys_str_mv | AT daleldaassi petroleumdegradingfungalisolatesforthetreatmentofsoilmicrocosms AT fatimahqabilalmaghrabi petroleumdegradingfungalisolatesforthetreatmentofsoilmicrocosms |