Standards for Quantitative Measurement of DNA Damage in Mammalian Cells
As the potential applications of DNA diagnostics continue to expand, there is a need for improved methods and standards for DNA analysis. This report describes several methods that could be considered for the production of reference materials for the quantitative measurement of DNA damage in mammali...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/6/5427 |
| _version_ | 1797611325781704704 |
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| author | Donald H. Atha Vytas Reipa |
| author_facet | Donald H. Atha Vytas Reipa |
| author_sort | Donald H. Atha |
| collection | DOAJ |
| description | As the potential applications of DNA diagnostics continue to expand, there is a need for improved methods and standards for DNA analysis. This report describes several methods that could be considered for the production of reference materials for the quantitative measurement of DNA damage in mammalian cells. With the focus on DNA strand breaks, potentially useful methods for assessing DNA damage in mammalian cells are reviewed. The advantages and limitations of each method, as well as additional concerns with respect to reference material development, are also discussed. In conclusion, we outline strategies for developing candidate DNA damage reference materials that could be adopted by research laboratories in a wide variety of applications. |
| first_indexed | 2024-03-11T06:26:19Z |
| format | Article |
| id | doaj.art-a956c5e9fcbb4faaad8fe0f7de870979 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:26:19Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a956c5e9fcbb4faaad8fe0f7de8709792023-11-17T11:33:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246542710.3390/ijms24065427Standards for Quantitative Measurement of DNA Damage in Mammalian CellsDonald H. Atha0Vytas Reipa1Materials Measurement Laboratory, National Institute of Standards and Technology, Biosystems and Biomaterials Division, Gaithersburg, MD 20899, USAMaterials Measurement Laboratory, National Institute of Standards and Technology, Biosystems and Biomaterials Division, Gaithersburg, MD 20899, USAAs the potential applications of DNA diagnostics continue to expand, there is a need for improved methods and standards for DNA analysis. This report describes several methods that could be considered for the production of reference materials for the quantitative measurement of DNA damage in mammalian cells. With the focus on DNA strand breaks, potentially useful methods for assessing DNA damage in mammalian cells are reviewed. The advantages and limitations of each method, as well as additional concerns with respect to reference material development, are also discussed. In conclusion, we outline strategies for developing candidate DNA damage reference materials that could be adopted by research laboratories in a wide variety of applications.https://www.mdpi.com/1422-0067/24/6/5427DNA damagestandardsreference materialsmammalian cellsgenotoxicity |
| spellingShingle | Donald H. Atha Vytas Reipa Standards for Quantitative Measurement of DNA Damage in Mammalian Cells International Journal of Molecular Sciences DNA damage standards reference materials mammalian cells genotoxicity |
| title | Standards for Quantitative Measurement of DNA Damage in Mammalian Cells |
| title_full | Standards for Quantitative Measurement of DNA Damage in Mammalian Cells |
| title_fullStr | Standards for Quantitative Measurement of DNA Damage in Mammalian Cells |
| title_full_unstemmed | Standards for Quantitative Measurement of DNA Damage in Mammalian Cells |
| title_short | Standards for Quantitative Measurement of DNA Damage in Mammalian Cells |
| title_sort | standards for quantitative measurement of dna damage in mammalian cells |
| topic | DNA damage standards reference materials mammalian cells genotoxicity |
| url | https://www.mdpi.com/1422-0067/24/6/5427 |
| work_keys_str_mv | AT donaldhatha standardsforquantitativemeasurementofdnadamageinmammaliancells AT vytasreipa standardsforquantitativemeasurementofdnadamageinmammaliancells |