Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor
mNGS(metagenomics Next Generation Sequencing), as a novel culture-independent approach, demonstrated the capability of rapid, sensitive, and accurate pathogen identification. At present, there have been many case reports about the use of mNGS to assist in the diagnosis of bacterial, fungal, viral an...
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| Format: | Article |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2021.682758/full |
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| author | YuChen Liu YuChen Liu Jun Zhang Bing Han LiJuan Du ZhaoYang Shi ChunCheng Wang Min Xu YongGang Luo |
| author_facet | YuChen Liu YuChen Liu Jun Zhang Bing Han LiJuan Du ZhaoYang Shi ChunCheng Wang Min Xu YongGang Luo |
| author_sort | YuChen Liu |
| collection | DOAJ |
| description | mNGS(metagenomics Next Generation Sequencing), as a novel culture-independent approach, demonstrated the capability of rapid, sensitive, and accurate pathogen identification. At present, there have been many case reports about the use of mNGS to assist in the diagnosis of bacterial, fungal, viral and parasitic infections and to guide clinicians to determine appropriate treatment. However, the clinical understanding of this technique is not comprehensive, and the experience of using it is relatively limited. We reported a 53-year-old man who was admitted to hospital with a high fever and headache. His inflammatory biomarkers were markedly elevated. Based on the clinical presentation, He was initially diagnosed as having an intracranial infection of unknown etiology and received empirical antibiotics and systemic supportive treatment. But these did not relieve his symptoms. Both the blood and CSF specimens were examined using traditional culture, serological testing, and mNGS. Traditional culture and serological testing produced negative results, while the mNGS revealed the presence of a potential pathogen, mucor, in the CSF specimen. Then targeted antifungal treatment was selected quickly and his temperature gradually returned to normal. Thus, we report the case in which mNGS was an auxiliary method to diagnose mucormycosis, and discuss this case in combination with relevant literature, in order to improve the clinical cognition of this technology. |
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| format | Article |
| id | doaj.art-a958209b7d6a488da9a6f3b053c86080 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-18T00:37:32Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj.art-a958209b7d6a488da9a6f3b053c860802022-12-21T21:26:58ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2021-09-01810.3389/fmed.2021.682758682758Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by MucorYuChen Liu0YuChen Liu1Jun Zhang2Bing Han3LiJuan Du4ZhaoYang Shi5ChunCheng Wang6Min Xu7YongGang Luo8Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaGene Hospital of Henan Province, Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinamNGS(metagenomics Next Generation Sequencing), as a novel culture-independent approach, demonstrated the capability of rapid, sensitive, and accurate pathogen identification. At present, there have been many case reports about the use of mNGS to assist in the diagnosis of bacterial, fungal, viral and parasitic infections and to guide clinicians to determine appropriate treatment. However, the clinical understanding of this technique is not comprehensive, and the experience of using it is relatively limited. We reported a 53-year-old man who was admitted to hospital with a high fever and headache. His inflammatory biomarkers were markedly elevated. Based on the clinical presentation, He was initially diagnosed as having an intracranial infection of unknown etiology and received empirical antibiotics and systemic supportive treatment. But these did not relieve his symptoms. Both the blood and CSF specimens were examined using traditional culture, serological testing, and mNGS. Traditional culture and serological testing produced negative results, while the mNGS revealed the presence of a potential pathogen, mucor, in the CSF specimen. Then targeted antifungal treatment was selected quickly and his temperature gradually returned to normal. Thus, we report the case in which mNGS was an auxiliary method to diagnose mucormycosis, and discuss this case in combination with relevant literature, in order to improve the clinical cognition of this technology.https://www.frontiersin.org/articles/10.3389/fmed.2021.682758/fullmucormycosisLichtheimia ramosametagenomics Next Generation Sequencingprecision treatmentintracranial infection |
| spellingShingle | YuChen Liu YuChen Liu Jun Zhang Bing Han LiJuan Du ZhaoYang Shi ChunCheng Wang Min Xu YongGang Luo Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor Frontiers in Medicine mucormycosis Lichtheimia ramosa metagenomics Next Generation Sequencing precision treatment intracranial infection |
| title | Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor |
| title_full | Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor |
| title_fullStr | Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor |
| title_full_unstemmed | Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor |
| title_short | Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor |
| title_sort | case report diagnostic value of metagenomics next generation sequencing in intracranial infection caused by mucor |
| topic | mucormycosis Lichtheimia ramosa metagenomics Next Generation Sequencing precision treatment intracranial infection |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2021.682758/full |
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