Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases

Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases

We examined the ability of two human cytosolic transaminases, aspartate aminotransferase (GOT1) and alanine aminotransferase (GPT), to transform their preferred substrates whilst discriminating against similar metabolites. This offers an opportunity to survey our current understanding of enzyme sele...

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Bibliographic Details
Main Authors: Alessio Peracchi, Eugenia Polverini
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Molecules
Subjects:
substrate specificity
molecular discrimination
limits of specificity
enzyme assays
transaminases
enzyme evolution
Online Access:https://www.mdpi.com/1420-3049/27/4/1398

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