Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination
Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and pre...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/1661 |
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| author | Cheng-Chao Zhu Chu-Xin Wang Chen-Ya Lu Jin-Dong Wang Yu Zhou Min Xiong Chang-Quan Zhang Qiao-Quan Liu Qian-Feng Li |
| author_facet | Cheng-Chao Zhu Chu-Xin Wang Chen-Ya Lu Jin-Dong Wang Yu Zhou Min Xiong Chang-Quan Zhang Qiao-Quan Liu Qian-Feng Li |
| author_sort | Cheng-Chao Zhu |
| collection | DOAJ |
| description | Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of <i>OsbZIP09</i> inhibited rice PHS. Analysis of the expression of <i>OsbZIP09</i> and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including <i>OsLOX2</i> and <i>Late Embryogenesis Abundant</i> (<i>LEA</i>) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and <i>OsbZIP09</i> mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination. |
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| language | English |
| last_indexed | 2024-03-09T05:17:24Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a95c61a230de4f3a96f47778531eda1d2023-12-03T12:44:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224166110.3390/ijms22041661Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed GerminationCheng-Chao Zhu0Chu-Xin Wang1Chen-Ya Lu2Jin-Dong Wang3Yu Zhou4Min Xiong5Chang-Quan Zhang6Qiao-Quan Liu7Qian-Feng Li8Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, ChinaSeed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of <i>OsbZIP09</i> inhibited rice PHS. Analysis of the expression of <i>OsbZIP09</i> and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including <i>OsLOX2</i> and <i>Late Embryogenesis Abundant</i> (<i>LEA</i>) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and <i>OsbZIP09</i> mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.https://www.mdpi.com/1422-0067/22/4/1661OsbZIP09preharvest sproutingABARNA-seqDAP-seqseed germination |
| spellingShingle | Cheng-Chao Zhu Chu-Xin Wang Chen-Ya Lu Jin-Dong Wang Yu Zhou Min Xiong Chang-Quan Zhang Qiao-Quan Liu Qian-Feng Li Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination International Journal of Molecular Sciences OsbZIP09 preharvest sprouting ABA RNA-seq DAP-seq seed germination |
| title | Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination |
| title_full | Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination |
| title_fullStr | Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination |
| title_short | Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination |
| title_sort | genome wide identification and expression analysis of osbzip09 target genes in rice reveal its mechanism of controlling seed germination |
| topic | OsbZIP09 preharvest sprouting ABA RNA-seq DAP-seq seed germination |
| url | https://www.mdpi.com/1422-0067/22/4/1661 |
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