Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus
Polysaccharides from marine clams perform various biological activities, whereas information on structure is scarce. Here, a water-soluble polysaccharide MMPX-B2 was isolated from Meretrix meretrix Linnaeus. The proposed structure was deduced through characterization and its immunological activity w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/14/1/6 |
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| author | Li Li Heng Li Jianying Qian Yongfeng He Jialin Zheng Zhenming Lu Zhenghong Xu Jinsong Shi |
| author_facet | Li Li Heng Li Jianying Qian Yongfeng He Jialin Zheng Zhenming Lu Zhenghong Xu Jinsong Shi |
| author_sort | Li Li |
| collection | DOAJ |
| description | Polysaccharides from marine clams perform various biological activities, whereas information on structure is scarce. Here, a water-soluble polysaccharide MMPX-B2 was isolated from Meretrix meretrix Linnaeus. The proposed structure was deduced through characterization and its immunological activity was investigated. MMPX-B2 consisted of d-glucose and d-galctose residues at a molar ratio of 3.51:1.00. The average molecular weight of MMPX-B2 was 510 kDa. This polysaccharide possessed a main chain of (1→4)-linked-α-d-glucopyranosyl residues, partially substituted at the C-6 position by a few terminal β-d-galactose residues or branched chains consisting of (1→3)-linked β-d-galactose residues. Preliminary immunological tests in vitro showed that MMPX-B2 could stimulate the murine macrophages to release various cytokines, and the structure-activity relationship was then established. The present study demonstrated the potential immunological activity of MMPX-B2, and provided references for studying the active ingredients in M. meretrix. |
| first_indexed | 2024-04-12T19:43:24Z |
| format | Article |
| id | doaj.art-a95cc21cf8ca428a9dc86c4c3d5c7ab4 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-12T19:43:24Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a95cc21cf8ca428a9dc86c4c3d5c7ab42022-12-22T03:19:02ZengMDPI AGMarine Drugs1660-33972015-12-01141610.3390/md14010006md14010006Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix LinnaeusLi Li0Heng Li1Jianying Qian2Yongfeng He3Jialin Zheng4Zhenming Lu5Zhenghong Xu6Jinsong Shi7School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Science, Jiangnan University, Wuxi 214122, ChinaPolysaccharides from marine clams perform various biological activities, whereas information on structure is scarce. Here, a water-soluble polysaccharide MMPX-B2 was isolated from Meretrix meretrix Linnaeus. The proposed structure was deduced through characterization and its immunological activity was investigated. MMPX-B2 consisted of d-glucose and d-galctose residues at a molar ratio of 3.51:1.00. The average molecular weight of MMPX-B2 was 510 kDa. This polysaccharide possessed a main chain of (1→4)-linked-α-d-glucopyranosyl residues, partially substituted at the C-6 position by a few terminal β-d-galactose residues or branched chains consisting of (1→3)-linked β-d-galactose residues. Preliminary immunological tests in vitro showed that MMPX-B2 could stimulate the murine macrophages to release various cytokines, and the structure-activity relationship was then established. The present study demonstrated the potential immunological activity of MMPX-B2, and provided references for studying the active ingredients in M. meretrix.http://www.mdpi.com/1660-3397/14/1/6Meretrix meretrix Linnaeuspolysaccharidestructureimmunological activity |
| spellingShingle | Li Li Heng Li Jianying Qian Yongfeng He Jialin Zheng Zhenming Lu Zhenghong Xu Jinsong Shi Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus Marine Drugs Meretrix meretrix Linnaeus polysaccharide structure immunological activity |
| title | Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus |
| title_full | Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus |
| title_fullStr | Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus |
| title_full_unstemmed | Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus |
| title_short | Structural and Immunological Activity Characterization of a Polysaccharide Isolated from Meretrix meretrix Linnaeus |
| title_sort | structural and immunological activity characterization of a polysaccharide isolated from meretrix meretrix linnaeus |
| topic | Meretrix meretrix Linnaeus polysaccharide structure immunological activity |
| url | http://www.mdpi.com/1660-3397/14/1/6 |
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