Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats
The present study was designed to investigate the antioxidant property of l -carnitine (LC) on serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (TH) and testis oxidative stress in streptozotocin (STZ)-induced diabetic rats. The rats were divided into the fol...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-08-01
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| Series: | Journal of Evidence-Based Integrative Medicine |
| Online Access: | https://doi.org/10.1177/2515690X18796053 |
| _version_ | 1819049087870173184 |
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| author | Nourollah Rezaei PhD Tahereh Mardanshahi MSc Majid Malekzadeh Shafaroudi PhD Saeed Abedian PhD Hamid Mohammadi PhD Zohre Zare PhD |
| author_facet | Nourollah Rezaei PhD Tahereh Mardanshahi MSc Majid Malekzadeh Shafaroudi PhD Saeed Abedian PhD Hamid Mohammadi PhD Zohre Zare PhD |
| author_sort | Nourollah Rezaei PhD |
| collection | DOAJ |
| description | The present study was designed to investigate the antioxidant property of l -carnitine (LC) on serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (TH) and testis oxidative stress in streptozotocin (STZ)-induced diabetic rats. The rats were divided into the following groups: group I, control; group II, LC 100 mg/kg/d; group III, diabetic; and groups IV to VI, diabetic rats treated with 50, 100, and 200 mg/kg/d of LC, respectively. Daily injections were given intraperitoneally for 7 weeks. At the end of experimental period, after sacrificing the rats, FSH, LH, TH, total antioxidant capacity (TAC), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), mitochondrial function (MTT), protein carbonyl (PC), and reactive oxygen species (ROS) levels were measured. STZ caused an elevation of MDA, ROS, and PC ( P < .001) with reduction of GSH, CAT, TAC, and MTT ( P < .001) in the serum levels. Group VI had significantly increased FSH, LH, and TH levels versus the untreated diabetic group ( P < .001). Although groups V and VI significantly decreased MDA ( P < .001), PC ( P < .01), and ROS ( P < .01) compared with the untreated diabetic group; only in group VI, the activity of GSH ( P < .001), CAT ( P < .01), TAC ( P < .001), and MTT ( P < .001) significantly increased. The results of the present study suggest that LC decreased diabetes-induced oxidative stress complications and also improved serum level of FSH, LH, and TH by reducing levels of lipid peroxidation and increasing antioxidant enzymes. |
| first_indexed | 2024-12-21T11:26:35Z |
| format | Article |
| id | doaj.art-a95cc313685042679f112026b50370a2 |
| institution | Directory Open Access Journal |
| issn | 2515-690X |
| language | English |
| last_indexed | 2024-12-21T11:26:35Z |
| publishDate | 2018-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Evidence-Based Integrative Medicine |
| spelling | doaj.art-a95cc313685042679f112026b50370a22022-12-21T19:05:39ZengSAGE PublishingJournal of Evidence-Based Integrative Medicine2515-690X2018-08-012310.1177/2515690X18796053Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic RatsNourollah Rezaei PhD0Tahereh Mardanshahi MSc1Majid Malekzadeh Shafaroudi PhD2Saeed Abedian PhD3Hamid Mohammadi PhD4Zohre Zare PhD5 Mazandaran University of Medical Sciences, Sari, Iran Mazandaran University of Medical Sciences, Sari, Iran Mazandaran University of Medical Sciences, Sari, Iran Mazandaran University of Medical Sciences, Sari, Iran Mazandaran University of Medical Sciences, Sari, Iran Mazandaran University of Medical Sciences, Sari, IranThe present study was designed to investigate the antioxidant property of l -carnitine (LC) on serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (TH) and testis oxidative stress in streptozotocin (STZ)-induced diabetic rats. The rats were divided into the following groups: group I, control; group II, LC 100 mg/kg/d; group III, diabetic; and groups IV to VI, diabetic rats treated with 50, 100, and 200 mg/kg/d of LC, respectively. Daily injections were given intraperitoneally for 7 weeks. At the end of experimental period, after sacrificing the rats, FSH, LH, TH, total antioxidant capacity (TAC), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), mitochondrial function (MTT), protein carbonyl (PC), and reactive oxygen species (ROS) levels were measured. STZ caused an elevation of MDA, ROS, and PC ( P < .001) with reduction of GSH, CAT, TAC, and MTT ( P < .001) in the serum levels. Group VI had significantly increased FSH, LH, and TH levels versus the untreated diabetic group ( P < .001). Although groups V and VI significantly decreased MDA ( P < .001), PC ( P < .01), and ROS ( P < .01) compared with the untreated diabetic group; only in group VI, the activity of GSH ( P < .001), CAT ( P < .01), TAC ( P < .001), and MTT ( P < .001) significantly increased. The results of the present study suggest that LC decreased diabetes-induced oxidative stress complications and also improved serum level of FSH, LH, and TH by reducing levels of lipid peroxidation and increasing antioxidant enzymes.https://doi.org/10.1177/2515690X18796053 |
| spellingShingle | Nourollah Rezaei PhD Tahereh Mardanshahi MSc Majid Malekzadeh Shafaroudi PhD Saeed Abedian PhD Hamid Mohammadi PhD Zohre Zare PhD Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats Journal of Evidence-Based Integrative Medicine |
| title | Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats |
| title_full | Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats |
| title_fullStr | Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats |
| title_full_unstemmed | Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats |
| title_short | Effects of -Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats |
| title_sort | effects of carnitine on the follicle stimulating hormone luteinizing hormone testosterone and testicular tissue oxidative stress levels in streptozotocin induced diabetic rats |
| url | https://doi.org/10.1177/2515690X18796053 |
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