Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methods
Background: The upper airways of cystic fibrosis (CF) persons are an evolutionary niche where genetically adapted bacterial strains are selected for lung infection. The microbiological studies conducted up to now on the upper airways are not easily comparable. Methods: Using classical culture method...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024030093 |
| _version_ | 1827316482230452224 |
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| author | Daniela Dolce Novella Ravenni Cristina Fevola Michela Francalanci Paolo Bonomi Maria Chiara Cavicchi Valeria Galici Anna Silvia Neri Giovanni Taccetti Vito Terlizzi Diletta Innocenti Beatrice Ferrari Chiara Bianchimani Erica Camera Tommaso Orioli Silvia Campana |
| author_facet | Daniela Dolce Novella Ravenni Cristina Fevola Michela Francalanci Paolo Bonomi Maria Chiara Cavicchi Valeria Galici Anna Silvia Neri Giovanni Taccetti Vito Terlizzi Diletta Innocenti Beatrice Ferrari Chiara Bianchimani Erica Camera Tommaso Orioli Silvia Campana |
| author_sort | Daniela Dolce |
| collection | DOAJ |
| description | Background: The upper airways of cystic fibrosis (CF) persons are an evolutionary niche where genetically adapted bacterial strains are selected for lung infection. The microbiological studies conducted up to now on the upper airways are not easily comparable. Methods: Using classical culture methods, we simultaneously studied the microbiological status of upper and lower airways in persons not chronically infected with P. aeruginosa. Each person had a single upper airways sampling and a concomitant lower airways sampling. Lower airways sampling was performed by oropharyngeal swab or sputum collection. Using a quasi-experimental design of study, we evaluated the performance of 2 different upper airways’ sampling methods, nasal lavage according to method described by Mainz or nasal lavage with a rhino-set. Pain was measured with appropriate scales. Results: A total of 194 persons were enrolled in this study. Pathogenic flora was found in 128 (6.6%) of 194 upper airways samples and in 164 (84.6%) lower airways samples. A statistically significant difference between the upper airways and the lower airways was found in the isolation of S. aureus and non-fermenter gram negatives. Nasal lavage according to Mainz resulted in the isolation of more non-fermenter gramnegatives than the rhino-set (p < 0.05). No differences were found in the pain caused bythe two methods. Conclusions: In our study population, cultures of the upper airway and lower airway differ in CF persons. In people sampled with nasal lavage according to Mainz more non-fermenter gram negatives were detected than with rhino-set. The two sampling methods were comparable with regard to the caused pain, nasal lavage according to Mainz method being quicker to perform. |
| first_indexed | 2024-03-07T15:46:43Z |
| format | Article |
| id | doaj.art-a95d21a0cdc24eabb44dcdf92becc667 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:15:13Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-a95d21a0cdc24eabb44dcdf92becc6672024-03-17T07:56:57ZengElsevierHeliyon2405-84402024-03-01105e26978Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methodsDaniela Dolce0Novella Ravenni1Cristina Fevola2Michela Francalanci3Paolo Bonomi4Maria Chiara Cavicchi5Valeria Galici6Anna Silvia Neri7Giovanni Taccetti8Vito Terlizzi9Diletta Innocenti10Beatrice Ferrari11Chiara Bianchimani12Erica Camera13Tommaso Orioli14Silvia Campana15Meyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyFreelance Statistician, Milan, 20146, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, Italy; Corresponding author.Meyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Rehabilitation Unit, Florence, ItalyMeyer Children's Hospital IRCCS, Rehabilitation Unit, Florence, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyMeyer Children's Hospital IRCCS, Cystic Fibrosis Regional Reference Centre, Department of Paediatric Medicine, Florence, 50139, ItalyBackground: The upper airways of cystic fibrosis (CF) persons are an evolutionary niche where genetically adapted bacterial strains are selected for lung infection. The microbiological studies conducted up to now on the upper airways are not easily comparable. Methods: Using classical culture methods, we simultaneously studied the microbiological status of upper and lower airways in persons not chronically infected with P. aeruginosa. Each person had a single upper airways sampling and a concomitant lower airways sampling. Lower airways sampling was performed by oropharyngeal swab or sputum collection. Using a quasi-experimental design of study, we evaluated the performance of 2 different upper airways’ sampling methods, nasal lavage according to method described by Mainz or nasal lavage with a rhino-set. Pain was measured with appropriate scales. Results: A total of 194 persons were enrolled in this study. Pathogenic flora was found in 128 (6.6%) of 194 upper airways samples and in 164 (84.6%) lower airways samples. A statistically significant difference between the upper airways and the lower airways was found in the isolation of S. aureus and non-fermenter gram negatives. Nasal lavage according to Mainz resulted in the isolation of more non-fermenter gramnegatives than the rhino-set (p < 0.05). No differences were found in the pain caused bythe two methods. Conclusions: In our study population, cultures of the upper airway and lower airway differ in CF persons. In people sampled with nasal lavage according to Mainz more non-fermenter gram negatives were detected than with rhino-set. The two sampling methods were comparable with regard to the caused pain, nasal lavage according to Mainz method being quicker to perform.http://www.sciencedirect.com/science/article/pii/S2405844024030093 |
| spellingShingle | Daniela Dolce Novella Ravenni Cristina Fevola Michela Francalanci Paolo Bonomi Maria Chiara Cavicchi Valeria Galici Anna Silvia Neri Giovanni Taccetti Vito Terlizzi Diletta Innocenti Beatrice Ferrari Chiara Bianchimani Erica Camera Tommaso Orioli Silvia Campana Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methods Heliyon |
| title | Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methods |
| title_full | Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methods |
| title_fullStr | Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methods |
| title_full_unstemmed | Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methods |
| title_short | Microbiology of cystic fibrosis persons not chronically infected with P. aeruginosa: A quasi-experimental study on two different upper airways’ sampling methods |
| title_sort | microbiology of cystic fibrosis persons not chronically infected with p aeruginosa a quasi experimental study on two different upper airways sampling methods |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024030093 |
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