Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum
Abstract The mycobiome, comprising fungi inhabiting plants, potentially plays a crucial role in tree health and survival amidst environmental stressors like climate change and pathogenic fungi. Understanding the intricate relationships between trees and their microbial communities is essential for d...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-03099-y |
| _version_ | 1797577765012111360 |
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| author | Blessing Durodola Kathrin Blumenstein Adedolapo Akinbobola Anna Kolehmainen Victor Chano Oliver Gailing Eeva Terhonen |
| author_facet | Blessing Durodola Kathrin Blumenstein Adedolapo Akinbobola Anna Kolehmainen Victor Chano Oliver Gailing Eeva Terhonen |
| author_sort | Blessing Durodola |
| collection | DOAJ |
| description | Abstract The mycobiome, comprising fungi inhabiting plants, potentially plays a crucial role in tree health and survival amidst environmental stressors like climate change and pathogenic fungi. Understanding the intricate relationships between trees and their microbial communities is essential for developing effective strategies to bolster the resilience and well-being of forest ecosystems as we adopt more sustainable forest management practices. The mycobiome can be considered an integral aspect of a tree’s biology, closely linked to its genotype. To explore the influence of host genetics and environmental factors on fungal composition, we examined the mycobiome associated with phloem and roots of Norway spruce (Picea abies (L.) Karst.) cuttings under varying watering conditions. To test the “mycobiome-associated-fitness” hypothesis, we compared seedlings artificially inoculated with Heterobasidion parviporum and control plants to evaluate mycobiome interaction on necrosis development. We aimed to 1) identify specific mycobiome species for the Norway spruce genotypes/families within the phloem and root tissues and their interactions with H. parviporum and 2) assess stability in the mycobiome species composition under abiotic disturbances (reduced water availability). The mycobiome was analyzed by sequencing the ribosomal ITS2 region. Our results revealed significant variations in the diversity and prevalence of the phloem mycobiome among different Norway spruce genotypes, highlighting the considerable impact of genetic variation on the composition and diversity of the phloem mycobiome. Additionally, specific mycobiome genera in the phloem showed variations in response to water availability, indicating the influence of environmental conditions on the relative proportion of certain fungal genera in Norway spruce trees. In the root mycobiome, key fungi such as Phialocephala fortinii and Paraphaeosphaeria neglecta were identified as conferring inhibitory effects against H. parviporum growth in Norway spruce genotypes. Furthermore, certain endophytes demonstrated greater stability in root ecosystems under low water conditions than ectomycorrhizal fungi. This knowledge can contribute to developing sustainable forest management practices that enhance the well-being of trees and their ecosystems, ultimately bolstering forest resilience. |
| first_indexed | 2024-03-10T22:12:39Z |
| format | Article |
| id | doaj.art-a9603a3be1c44a068f742cf38157a337 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-10T22:12:39Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a9603a3be1c44a068f742cf38157a3372023-11-19T12:33:01ZengBMCBMC Microbiology1471-21802023-11-0123112110.1186/s12866-023-03099-yBeyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporumBlessing Durodola0Kathrin Blumenstein1Adedolapo Akinbobola2Anna Kolehmainen3Victor Chano4Oliver Gailing5Eeva Terhonen6Forest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of GöttingenForest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of GöttingenForest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of GöttingenForest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of GöttingenDepartment of Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Faculty of Forest Sciences and Forest Ecology, University of GöttingenDepartment of Forest Genetics and Forest Tree Breeding, Büsgen-Institute, Faculty of Forest Sciences and Forest Ecology, University of GöttingenForest Pathology Research Group, Büsgen-Institute, Department of Forest Botany and Tree Physiology, Faculty of Forest Sciences and Forest Ecology, University of GöttingenAbstract The mycobiome, comprising fungi inhabiting plants, potentially plays a crucial role in tree health and survival amidst environmental stressors like climate change and pathogenic fungi. Understanding the intricate relationships between trees and their microbial communities is essential for developing effective strategies to bolster the resilience and well-being of forest ecosystems as we adopt more sustainable forest management practices. The mycobiome can be considered an integral aspect of a tree’s biology, closely linked to its genotype. To explore the influence of host genetics and environmental factors on fungal composition, we examined the mycobiome associated with phloem and roots of Norway spruce (Picea abies (L.) Karst.) cuttings under varying watering conditions. To test the “mycobiome-associated-fitness” hypothesis, we compared seedlings artificially inoculated with Heterobasidion parviporum and control plants to evaluate mycobiome interaction on necrosis development. We aimed to 1) identify specific mycobiome species for the Norway spruce genotypes/families within the phloem and root tissues and their interactions with H. parviporum and 2) assess stability in the mycobiome species composition under abiotic disturbances (reduced water availability). The mycobiome was analyzed by sequencing the ribosomal ITS2 region. Our results revealed significant variations in the diversity and prevalence of the phloem mycobiome among different Norway spruce genotypes, highlighting the considerable impact of genetic variation on the composition and diversity of the phloem mycobiome. Additionally, specific mycobiome genera in the phloem showed variations in response to water availability, indicating the influence of environmental conditions on the relative proportion of certain fungal genera in Norway spruce trees. In the root mycobiome, key fungi such as Phialocephala fortinii and Paraphaeosphaeria neglecta were identified as conferring inhibitory effects against H. parviporum growth in Norway spruce genotypes. Furthermore, certain endophytes demonstrated greater stability in root ecosystems under low water conditions than ectomycorrhizal fungi. This knowledge can contribute to developing sustainable forest management practices that enhance the well-being of trees and their ecosystems, ultimately bolstering forest resilience.https://doi.org/10.1186/s12866-023-03099-yPicea abiesPlant-host relationshipDrought stressGenotypic variationMicrobesFungal community |
| spellingShingle | Blessing Durodola Kathrin Blumenstein Adedolapo Akinbobola Anna Kolehmainen Victor Chano Oliver Gailing Eeva Terhonen Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum BMC Microbiology Picea abies Plant-host relationship Drought stress Genotypic variation Microbes Fungal community |
| title | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
| title_full | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
| title_fullStr | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
| title_full_unstemmed | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
| title_short | Beyond the surface: exploring the mycobiome of Norway spruce under drought stress and with Heterobasidion parviporum |
| title_sort | beyond the surface exploring the mycobiome of norway spruce under drought stress and with heterobasidion parviporum |
| topic | Picea abies Plant-host relationship Drought stress Genotypic variation Microbes Fungal community |
| url | https://doi.org/10.1186/s12866-023-03099-y |
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