The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer
Background NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer,...
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PeerJ Inc.
2023-10-01
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| Online Access: | https://peerj.com/articles/16263.pdf |
| _version_ | 1797419719675871232 |
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| author | Lifan Tan Wensong Li Qin Su |
| author_facet | Lifan Tan Wensong Li Qin Su |
| author_sort | Lifan Tan |
| collection | DOAJ |
| description | Background NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis. Methods To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis. Results Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells. Conclusion High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment. |
| first_indexed | 2024-03-09T06:51:25Z |
| format | Article |
| id | doaj.art-a96078a1ab4846eb8589f6199f90328e |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:51:25Z |
| publishDate | 2023-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-a96078a1ab4846eb8589f6199f90328e2023-12-03T10:26:13ZengPeerJ Inc.PeerJ2167-83592023-10-0111e1626310.7717/peerj.16263The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancerLifan Tan0Wensong Li1Qin Su2Department of Otolaryngology, West China-Guang’an Hospital, Sichuan University, Guang’an, Sichuan, ChinaDepartment of Otolaryngology, West China-Guang’an Hospital, Sichuan University, Guang’an, Sichuan, ChinaDepartment of Otolaryngology, The People’s Hospital of Dujiangyan, Dujiangyan, Sichuan, ChinaBackground NTMT1, a transfer methylase that adds methyl groups to the N-terminus of proteins, has been identified as a critical player in tumor development and progression. However, its precise function in pan-cancer is still unclear. To gain a more comprehensive understanding of its role in cancer, we performed a thorough bioinformatics analysis. Methods To conduct our analysis, we gathered data from multiple sources, including RNA sequencing and clinical data from the TCGA database, protein expression data from the UALCAN and HPA databases, and single-cell expression data from the CancerSEA database. Additionally, we utilized TISIDB to investigate the interaction between the tumor and the immune system. To assess the impact of NTMT1 on the proliferation of SNU1076 cells, we performed a CCK8 assay. We also employed cellular immunofluorescence to detect DNA damage and used flow cytometry to measure tumor cell apoptosis. Results Our analysis revealed that NTMT1 was significantly overexpressed in various types of tumors and that high levels of NTMT1 were associated with poor survival outcomes. Functional enrichment analysis indicated that NTMT1 may contribute to tumor development and progression by regulating pathways involved in cell proliferation and immune response. In addition, we found that knockdown of NTMT1 expression led to reduced cell proliferation, increased DNA damage, and enhanced apoptosis in HNSCC cells. Conclusion High expression of NTMT1 in tumors is associated with poor prognosis. The underlying regulatory mechanism of NTMT1 in cancer is complex, and it may be involved in both the promotion of tumor development and the inhibition of the tumor immune microenvironment.https://peerj.com/articles/16263.pdfNTMT1Pan-cancerBioinformatics analysisPrognosticHead and neck squamous cell carcinoma |
| spellingShingle | Lifan Tan Wensong Li Qin Su The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer PeerJ NTMT1 Pan-cancer Bioinformatics analysis Prognostic Head and neck squamous cell carcinoma |
| title | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
| title_full | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
| title_fullStr | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
| title_full_unstemmed | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
| title_short | The comprehensive analysis of the prognostic and functional role of N-terminal methyltransferases 1 in pan-cancer |
| title_sort | comprehensive analysis of the prognostic and functional role of n terminal methyltransferases 1 in pan cancer |
| topic | NTMT1 Pan-cancer Bioinformatics analysis Prognostic Head and neck squamous cell carcinoma |
| url | https://peerj.com/articles/16263.pdf |
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