THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIA
This study was aimed to detect the prevalence of blaVIM and blaNDM genes among Enterobacter cloacae isolates that were isolated from Iraqi patients. About 50 bacterial isolates were collected from different hospitals in Baghdad city and all these isolates were diagnosed using biochemical tests and...
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| Format: | Article |
| Language: | English |
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Baghdad University
2022-08-01
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| Series: | The Iraqi Journal of Agricultural science |
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| Online Access: | https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1608 |
| _version_ | 1797999829201190912 |
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| author | Suhad S. Mahmood |
| author_facet | Suhad S. Mahmood |
| author_sort | Suhad S. Mahmood |
| collection | DOAJ |
| description |
This study was aimed to detect the prevalence of blaVIM and blaNDM genes among Enterobacter cloacae isolates that were isolated from Iraqi patients. About 50 bacterial isolates were collected from different hospitals in Baghdad city and all these isolates were diagnosed using biochemical tests and CHROMagar culture media and conformed using the Vitek II system. The antibiotic susceptibility for E.cloacae isolates was determined using the disk diffusion (Kirby Bauer) method and the results showed that these bacteria were showed resistance to Cefepime and Meropenem antibiotics in percentage (50%), (40%) respectively. In addition it is resistant to Amikacin, ampicillin/sulbactam, Piperacillin, and aztreonam was (20%), (50%), (40%), and (55%) respectively. Also it has been noted that all isolates showed resistance to Cefixime in percentage (100%). EDTA combined disc test was used to detect the ability of E. cloacae isolates to produce carbapenemase and the results were showed that only 8 isolates were gave positive results , While the prevalence of blaVIM and blaNDM was determined using the conventional Polymerase chain reaction technique. And the result showed that only four isolates harbored blaNDM while blaVIM was present in 3 isolates
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| first_indexed | 2024-04-11T11:10:57Z |
| format | Article |
| id | doaj.art-a9620f1a5dbc4ac4ac81d56204bccedc |
| institution | Directory Open Access Journal |
| issn | 0075-0530 2410-0862 |
| language | English |
| last_indexed | 2024-04-11T11:10:57Z |
| publishDate | 2022-08-01 |
| publisher | Baghdad University |
| record_format | Article |
| series | The Iraqi Journal of Agricultural science |
| spelling | doaj.art-a9620f1a5dbc4ac4ac81d56204bccedc2022-12-22T04:27:33ZengBaghdad UniversityThe Iraqi Journal of Agricultural science0075-05302410-08622022-08-0153410.36103/ijas.v53i4.1608THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIASuhad S. Mahmood This study was aimed to detect the prevalence of blaVIM and blaNDM genes among Enterobacter cloacae isolates that were isolated from Iraqi patients. About 50 bacterial isolates were collected from different hospitals in Baghdad city and all these isolates were diagnosed using biochemical tests and CHROMagar culture media and conformed using the Vitek II system. The antibiotic susceptibility for E.cloacae isolates was determined using the disk diffusion (Kirby Bauer) method and the results showed that these bacteria were showed resistance to Cefepime and Meropenem antibiotics in percentage (50%), (40%) respectively. In addition it is resistant to Amikacin, ampicillin/sulbactam, Piperacillin, and aztreonam was (20%), (50%), (40%), and (55%) respectively. Also it has been noted that all isolates showed resistance to Cefixime in percentage (100%). EDTA combined disc test was used to detect the ability of E. cloacae isolates to produce carbapenemase and the results were showed that only 8 isolates were gave positive results , While the prevalence of blaVIM and blaNDM was determined using the conventional Polymerase chain reaction technique. And the result showed that only four isolates harbored blaNDM while blaVIM was present in 3 isolates https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1608Enterobacteriace, carbapenem resistance, metallo β lactmase, EDTA combined disc test |
| spellingShingle | Suhad S. Mahmood THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIA The Iraqi Journal of Agricultural science Enterobacteriace, carbapenem resistance, metallo β lactmase, EDTA combined disc test |
| title | THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIA |
| title_full | THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIA |
| title_fullStr | THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIA |
| title_full_unstemmed | THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIA |
| title_short | THE PREVALENCE OF BLANDM, BLAVIM GENES AMONG ENTEROBACTER CLOACAE BACTERIA |
| title_sort | prevalence of blandm blavim genes among enterobacter cloacae bacteria |
| topic | Enterobacteriace, carbapenem resistance, metallo β lactmase, EDTA combined disc test |
| url | https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1608 |
| work_keys_str_mv | AT suhadsmahmood theprevalenceofblandmblavimgenesamongenterobactercloacaebacteria AT suhadsmahmood prevalenceofblandmblavimgenesamongenterobactercloacaebacteria |