Involvement of the miR156/SPL module in flooding response in Medicago sativa
Abstract The highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82450-7 |
| _version_ | 1818839956576010240 |
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| author | Biruk A. Feyissa Lisa Amyot Vida Nasrollahi Yousef Papadopoulos Susanne E. Kohalmi Abdelali Hannoufa |
| author_facet | Biruk A. Feyissa Lisa Amyot Vida Nasrollahi Yousef Papadopoulos Susanne E. Kohalmi Abdelali Hannoufa |
| author_sort | Biruk A. Feyissa |
| collection | DOAJ |
| description | Abstract The highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription factors. Here we investigated the involvement and possible mechanism of action of the miR156/SPL module in flooding tolerance in alfalfa. For that, we used miR156 overexpressing, SPL13RNAi, flood-tolerant (AAC-Trueman) and -sensitive (AC-Caribou) alfalfa cultivars exposed to flooding. We also used Arabidopsis ABA insensitive (abi1-2, abi5-8) mutants and transgenic lines with either overexpressed (KIN10-OX1, KIN10-OX2) or silenced (KIN10RNAi-1, KIN10RNAi-2) catalytic subunit of SnRK1 to investigate a possible role of ABA and SnRK1 in regulating miR156 expression under flooding. Physiological analysis, hormone profiling and global transcriptome changes revealed a role for miR156/SPL module in flooding tolerance. We also identified nine novel alfalfa SPLs (SPL1, SPL1a, SPL2a, SPL7, SPL7a, SPL8, SPL13a, SPL14, SPL16) responsive to flooding. Our results also showed a possible ABA-dependent SnRK1 upregulation to enhance miR156 expression, resulting in downregulation of SPL4, SPL7a, SPL8, SPL9, SPL13, and SPL13a. We conclude that these effects induce flooding adaptive responses in alfalfa and modulate stress physiology by affecting the transcriptome, ABA metabolites and secondary metabolism. |
| first_indexed | 2024-12-19T04:02:32Z |
| format | Article |
| id | doaj.art-a964fb14641e4d358983f1dc151183fe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:02:32Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a964fb14641e4d358983f1dc151183fe2022-12-21T20:36:39ZengNature PortfolioScientific Reports2045-23222021-02-0111111610.1038/s41598-021-82450-7Involvement of the miR156/SPL module in flooding response in Medicago sativaBiruk A. Feyissa0Lisa Amyot1Vida Nasrollahi2Yousef Papadopoulos3Susanne E. Kohalmi4Abdelali Hannoufa5Agriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaAgriculture and Agri-Food CanadaDepartment of Biology, University of Western OntarioAgriculture and Agri-Food CanadaAbstract The highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription factors. Here we investigated the involvement and possible mechanism of action of the miR156/SPL module in flooding tolerance in alfalfa. For that, we used miR156 overexpressing, SPL13RNAi, flood-tolerant (AAC-Trueman) and -sensitive (AC-Caribou) alfalfa cultivars exposed to flooding. We also used Arabidopsis ABA insensitive (abi1-2, abi5-8) mutants and transgenic lines with either overexpressed (KIN10-OX1, KIN10-OX2) or silenced (KIN10RNAi-1, KIN10RNAi-2) catalytic subunit of SnRK1 to investigate a possible role of ABA and SnRK1 in regulating miR156 expression under flooding. Physiological analysis, hormone profiling and global transcriptome changes revealed a role for miR156/SPL module in flooding tolerance. We also identified nine novel alfalfa SPLs (SPL1, SPL1a, SPL2a, SPL7, SPL7a, SPL8, SPL13a, SPL14, SPL16) responsive to flooding. Our results also showed a possible ABA-dependent SnRK1 upregulation to enhance miR156 expression, resulting in downregulation of SPL4, SPL7a, SPL8, SPL9, SPL13, and SPL13a. We conclude that these effects induce flooding adaptive responses in alfalfa and modulate stress physiology by affecting the transcriptome, ABA metabolites and secondary metabolism.https://doi.org/10.1038/s41598-021-82450-7 |
| spellingShingle | Biruk A. Feyissa Lisa Amyot Vida Nasrollahi Yousef Papadopoulos Susanne E. Kohalmi Abdelali Hannoufa Involvement of the miR156/SPL module in flooding response in Medicago sativa Scientific Reports |
| title | Involvement of the miR156/SPL module in flooding response in Medicago sativa |
| title_full | Involvement of the miR156/SPL module in flooding response in Medicago sativa |
| title_fullStr | Involvement of the miR156/SPL module in flooding response in Medicago sativa |
| title_full_unstemmed | Involvement of the miR156/SPL module in flooding response in Medicago sativa |
| title_short | Involvement of the miR156/SPL module in flooding response in Medicago sativa |
| title_sort | involvement of the mir156 spl module in flooding response in medicago sativa |
| url | https://doi.org/10.1038/s41598-021-82450-7 |
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