Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques
The increasing interest and recent developments in nanotechnology pose previously unparalleled challenges in understanding the effects of nanoparticles on living tissues. Despite significant progress in in vitro cell and tissue culture technologies, observations on particle distribution and tissue r...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Beilstein-Institut
2015-01-01
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| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.6.25 |
| _version_ | 1811203343356264448 |
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| author | Anja Ostrowski Daniel Nordmeyer Alexander Boreham Cornelia Holzhausen Lars Mundhenk Christina Graf Martina C. Meinke Annika Vogt Sabrina Hadam Jürgen Lademann Eckart Rühl Ulrike Alexiev Achim D. Gruber |
| author_facet | Anja Ostrowski Daniel Nordmeyer Alexander Boreham Cornelia Holzhausen Lars Mundhenk Christina Graf Martina C. Meinke Annika Vogt Sabrina Hadam Jürgen Lademann Eckart Rühl Ulrike Alexiev Achim D. Gruber |
| author_sort | Anja Ostrowski |
| collection | DOAJ |
| description | The increasing interest and recent developments in nanotechnology pose previously unparalleled challenges in understanding the effects of nanoparticles on living tissues. Despite significant progress in in vitro cell and tissue culture technologies, observations on particle distribution and tissue responses in whole organisms are still indispensable. In addition to a thorough understanding of complex tissue responses which is the domain of expert pathologists, the localization of particles at their sites of interaction with living structures is essential to complete the picture. In this review we will describe and compare different imaging techniques for localizing inorganic as well as organic nanoparticles in tissues, cells and subcellular compartments. The visualization techniques include well-established methods, such as standard light, fluorescence, transmission electron and scanning electron microscopy as well as more recent developments, such as light and electron microscopic autoradiography, fluorescence lifetime imaging, spectral imaging and linear unmixing, superresolution structured illumination, Raman microspectroscopy and X-ray microscopy. Importantly, all methodologies described allow for the simultaneous visualization of nanoparticles and evaluation of cell and tissue changes that are of prime interest for toxicopathologic studies. However, the different approaches vary in terms of applicability for specific particles, sensitivity, optical resolution, technical requirements and thus availability, and effects of labeling on particle properties. Specific bottle necks of each technology are discussed in detail. Interpretation of particle localization data from any of these techniques should therefore respect their specific merits and limitations as no single approach combines all desired properties. |
| first_indexed | 2024-04-12T02:54:19Z |
| format | Article |
| id | doaj.art-a966dc50b7924f5f934c054740693422 |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-04-12T02:54:19Z |
| publishDate | 2015-01-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-a966dc50b7924f5f934c0547406934222022-12-22T03:50:52ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862015-01-016126328010.3762/bjnano.6.252190-4286-6-25Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniquesAnja Ostrowski0Daniel Nordmeyer1Alexander Boreham2Cornelia Holzhausen3Lars Mundhenk4Christina Graf5Martina C. Meinke6Annika Vogt7Sabrina Hadam8Jürgen Lademann9Eckart Rühl10Ulrike Alexiev11Achim D. Gruber12Institute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, GermanyInstitute of Chemistry and Biochemistry - Physical and Theoretical Chemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, GermanyDepartment of Physics, Institute of Experimental Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, GermanyInstitute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, GermanyInstitute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, GermanyInstitute of Chemistry and Biochemistry - Physical and Theoretical Chemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, GermanyDepartment of Dermatology, Charite - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, GermanyDepartment of Dermatology, Charite - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, GermanyDepartment of Dermatology, Charite - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, GermanyDepartment of Dermatology, Charite - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, GermanyInstitute of Chemistry and Biochemistry - Physical and Theoretical Chemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, GermanyDepartment of Physics, Institute of Experimental Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, GermanyInstitute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, GermanyThe increasing interest and recent developments in nanotechnology pose previously unparalleled challenges in understanding the effects of nanoparticles on living tissues. Despite significant progress in in vitro cell and tissue culture technologies, observations on particle distribution and tissue responses in whole organisms are still indispensable. In addition to a thorough understanding of complex tissue responses which is the domain of expert pathologists, the localization of particles at their sites of interaction with living structures is essential to complete the picture. In this review we will describe and compare different imaging techniques for localizing inorganic as well as organic nanoparticles in tissues, cells and subcellular compartments. The visualization techniques include well-established methods, such as standard light, fluorescence, transmission electron and scanning electron microscopy as well as more recent developments, such as light and electron microscopic autoradiography, fluorescence lifetime imaging, spectral imaging and linear unmixing, superresolution structured illumination, Raman microspectroscopy and X-ray microscopy. Importantly, all methodologies described allow for the simultaneous visualization of nanoparticles and evaluation of cell and tissue changes that are of prime interest for toxicopathologic studies. However, the different approaches vary in terms of applicability for specific particles, sensitivity, optical resolution, technical requirements and thus availability, and effects of labeling on particle properties. Specific bottle necks of each technology are discussed in detail. Interpretation of particle localization data from any of these techniques should therefore respect their specific merits and limitations as no single approach combines all desired properties.https://doi.org/10.3762/bjnano.6.25fluorescence lifetime imagingfluorescence microscopyhistopathologylight microscopic autoradiographystructured illumination microscopy |
| spellingShingle | Anja Ostrowski Daniel Nordmeyer Alexander Boreham Cornelia Holzhausen Lars Mundhenk Christina Graf Martina C. Meinke Annika Vogt Sabrina Hadam Jürgen Lademann Eckart Rühl Ulrike Alexiev Achim D. Gruber Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques Beilstein Journal of Nanotechnology fluorescence lifetime imaging fluorescence microscopy histopathology light microscopic autoradiography structured illumination microscopy |
| title | Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques |
| title_full | Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques |
| title_fullStr | Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques |
| title_full_unstemmed | Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques |
| title_short | Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques |
| title_sort | overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques |
| topic | fluorescence lifetime imaging fluorescence microscopy histopathology light microscopic autoradiography structured illumination microscopy |
| url | https://doi.org/10.3762/bjnano.6.25 |
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