Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately
The present study aimed to clarify the potential mechanism of fucoidans found in <i>Ascophyllum nodosum</i> on anti-inflammation and to further explore the relationship between their structures and anti-inflammation. Two novel fucoidans named ANP-6 and ANP-7 and found in <i>A. nodo...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/15/2381 |
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| author | Lilong Wang Linlin Wang Chunhong Yan Chunqing Ai Chengrong Wen Xiaoming Guo Shuang Song |
| author_facet | Lilong Wang Linlin Wang Chunhong Yan Chunqing Ai Chengrong Wen Xiaoming Guo Shuang Song |
| author_sort | Lilong Wang |
| collection | DOAJ |
| description | The present study aimed to clarify the potential mechanism of fucoidans found in <i>Ascophyllum nodosum</i> on anti-inflammation and to further explore the relationship between their structures and anti-inflammation. Two novel fucoidans named ANP-6 and ANP-7 and found in <i>A. nodosum</i>, were separated and purified and their structures were elucidated by HPGPC, HPLC, GC-MS, FT-IR, NMR, and by the Congo red test. They both possessed a backbone constructed of →2)-α-L-Fuc<i>p</i>4<i>S</i>-(1→, →3)-α-L-Fuc<i>p</i>2<i>S</i>4<i>S</i>-(1→, →6)-β-D-Gal<i>p</i>-(1→, and →3,6)-β-D-Gal<i>p</i>4<i>S</i>-(1→ with branches of →2)-α-L-Fuc<i>p</i>4<i>S</i>-(1→ and →3)-β-D-Gal<i>p</i>-(1→. Moreover, ANP-6 and ANP-7 could prevent the inflammation of the LPS-stimulated macrophages by suppressing the NO production and by regulating the expressions of iNOS, COX-2, TNF-α, IL-1β, IL-6, and IL-10. Their inhibitory effects on the TLR-2 and TLR-4 levels suggest that they inhibit the inflammation process via the blocking of the TLR/NF-κB signal transduction. In addition, ANP-6, with a molecular weight (63.2 kDa), exhibited stronger anti-inflammatory capabilities than ANP-7 (124.5 kDa), thereby indicating that the molecular weight has an influence on the anti-inflammatory effects of fucoidans. |
| first_indexed | 2024-03-09T12:37:10Z |
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| id | doaj.art-a96750d647674fbf86e29644a61aaa91 |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T12:37:10Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a96750d647674fbf86e29644a61aaa912023-11-30T22:23:23ZengMDPI AGFoods2304-81582022-08-011115238110.3390/foods11152381Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway DiscriminatelyLilong Wang0Linlin Wang1Chunhong Yan2Chunqing Ai3Chengrong Wen4Xiaoming Guo5Shuang Song6National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaShenzhen Key Laboratory of Food Nutrition and Health, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaNational Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaThe present study aimed to clarify the potential mechanism of fucoidans found in <i>Ascophyllum nodosum</i> on anti-inflammation and to further explore the relationship between their structures and anti-inflammation. Two novel fucoidans named ANP-6 and ANP-7 and found in <i>A. nodosum</i>, were separated and purified and their structures were elucidated by HPGPC, HPLC, GC-MS, FT-IR, NMR, and by the Congo red test. They both possessed a backbone constructed of →2)-α-L-Fuc<i>p</i>4<i>S</i>-(1→, →3)-α-L-Fuc<i>p</i>2<i>S</i>4<i>S</i>-(1→, →6)-β-D-Gal<i>p</i>-(1→, and →3,6)-β-D-Gal<i>p</i>4<i>S</i>-(1→ with branches of →2)-α-L-Fuc<i>p</i>4<i>S</i>-(1→ and →3)-β-D-Gal<i>p</i>-(1→. Moreover, ANP-6 and ANP-7 could prevent the inflammation of the LPS-stimulated macrophages by suppressing the NO production and by regulating the expressions of iNOS, COX-2, TNF-α, IL-1β, IL-6, and IL-10. Their inhibitory effects on the TLR-2 and TLR-4 levels suggest that they inhibit the inflammation process via the blocking of the TLR/NF-κB signal transduction. In addition, ANP-6, with a molecular weight (63.2 kDa), exhibited stronger anti-inflammatory capabilities than ANP-7 (124.5 kDa), thereby indicating that the molecular weight has an influence on the anti-inflammatory effects of fucoidans.https://www.mdpi.com/2304-8158/11/15/2381sulfated polysaccharideschemical structureanti-inflammationmolecular weight |
| spellingShingle | Lilong Wang Linlin Wang Chunhong Yan Chunqing Ai Chengrong Wen Xiaoming Guo Shuang Song Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately Foods sulfated polysaccharides chemical structure anti-inflammation molecular weight |
| title | Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately |
| title_full | Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately |
| title_fullStr | Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately |
| title_full_unstemmed | Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately |
| title_short | Two <i>Ascophyllum nodosum</i> Fucoidans with Different Molecular Weights Inhibit Inflammation via Blocking of TLR/NF-κB Signaling Pathway Discriminately |
| title_sort | two i ascophyllum nodosum i fucoidans with different molecular weights inhibit inflammation via blocking of tlr nf κb signaling pathway discriminately |
| topic | sulfated polysaccharides chemical structure anti-inflammation molecular weight |
| url | https://www.mdpi.com/2304-8158/11/15/2381 |
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