Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i>
Arbuscular mycorrhizal fungi (AMF) are a group of soil microorganisms that establish symbioses with most land plant species. “Root trap culture” generally has been used for isolating a single regenerated spore in order to establish a monospecific, native AMF line. Roots may be co...
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MDPI AG
2019-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/6/142 |
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| author | Yoshihiro Kobae Ryo Ohtomo Sho Morimoto Daiki Sato Tomomi Nakagawa Norikuni Oka Shusei Sato |
| author_facet | Yoshihiro Kobae Ryo Ohtomo Sho Morimoto Daiki Sato Tomomi Nakagawa Norikuni Oka Shusei Sato |
| author_sort | Yoshihiro Kobae |
| collection | DOAJ |
| description | Arbuscular mycorrhizal fungi (AMF) are a group of soil microorganisms that establish symbioses with most land plant species. “Root trap culture” generally has been used for isolating a single regenerated spore in order to establish a monospecific, native AMF line. Roots may be co-colonized with multiple AMF species; however, only a small portion of AMF within roots sporulate, and do so only under certain conditions. In this study, we tested whether young thalli (<2 mm) of the liverwort <i>Marchantia paleacea</i> harbour monospecific AMF, and can be used as a vegetative inoculant line. When <i>M. paleacea</i> gemmae were co-cultivated with roots obtained from the field, the young thalli were infected by AMF via rhizoids and formed arbuscules after 18 days post-sowing. Ribosomal DNA sequencing of the AMF-colonized thalli (mycothalli) revealed that they harboured phylogenetically diverse AMF; however, new gemmae sown around transplanted mycothalli showed evidence of colonization from phylogenetically uniform <i>Rhizophagus</i> species. Of note, mycothalli can also be used as an inoculum. These results suggest that the young thalli of <i>M. paleacea</i> can potentially isolate monospecific AMF from field soil in a spore-independent manner. |
| first_indexed | 2024-04-11T19:47:21Z |
| format | Article |
| id | doaj.art-a9685a426bec428b9f8b1a642f5fbc29 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-11T19:47:21Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a9685a426bec428b9f8b1a642f5fbc292022-12-22T04:06:24ZengMDPI AGPlants2223-77472019-05-018614210.3390/plants8060142plants8060142Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i>Yoshihiro Kobae0Ryo Ohtomo1Sho Morimoto2Daiki Sato3Tomomi Nakagawa4Norikuni Oka5Shusei Sato6Laboratory of Crop Nutrition, Department of Sustainable Agriculture, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanHokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, JapanHokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, JapanLaboratory of Crop Nutrition, Department of Sustainable Agriculture, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, JapanDivision of Symbiotic Systems, National Institute for Basic Biology, Nishigonaka 38, Myodaiji, Okazaki 444-8585, Aichi, JapanHokkaido Agricultural Research Center, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, JapanGraduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-Ku, Sendai 980-8577, JapanArbuscular mycorrhizal fungi (AMF) are a group of soil microorganisms that establish symbioses with most land plant species. “Root trap culture” generally has been used for isolating a single regenerated spore in order to establish a monospecific, native AMF line. Roots may be co-colonized with multiple AMF species; however, only a small portion of AMF within roots sporulate, and do so only under certain conditions. In this study, we tested whether young thalli (<2 mm) of the liverwort <i>Marchantia paleacea</i> harbour monospecific AMF, and can be used as a vegetative inoculant line. When <i>M. paleacea</i> gemmae were co-cultivated with roots obtained from the field, the young thalli were infected by AMF via rhizoids and formed arbuscules after 18 days post-sowing. Ribosomal DNA sequencing of the AMF-colonized thalli (mycothalli) revealed that they harboured phylogenetically diverse AMF; however, new gemmae sown around transplanted mycothalli showed evidence of colonization from phylogenetically uniform <i>Rhizophagus</i> species. Of note, mycothalli can also be used as an inoculum. These results suggest that the young thalli of <i>M. paleacea</i> can potentially isolate monospecific AMF from field soil in a spore-independent manner.https://www.mdpi.com/2223-7747/8/6/142liverwort<i>Marchantia paleacea</i>mycothallinative arbuscular mycorrhizal fungiSanger sequencing |
| spellingShingle | Yoshihiro Kobae Ryo Ohtomo Sho Morimoto Daiki Sato Tomomi Nakagawa Norikuni Oka Shusei Sato Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i> Plants liverwort <i>Marchantia paleacea</i> mycothalli native arbuscular mycorrhizal fungi Sanger sequencing |
| title | Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i> |
| title_full | Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i> |
| title_fullStr | Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i> |
| title_full_unstemmed | Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i> |
| title_short | Isolation of Native Arbuscular Mycorrhizal Fungi within Young Thalli of the Liverwort <i>Marchantia paleacea</i> |
| title_sort | isolation of native arbuscular mycorrhizal fungi within young thalli of the liverwort i marchantia paleacea i |
| topic | liverwort <i>Marchantia paleacea</i> mycothalli native arbuscular mycorrhizal fungi Sanger sequencing |
| url | https://www.mdpi.com/2223-7747/8/6/142 |
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