Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana
Glutaredoxins (Grxs) are a family of small multifunctional proteins involved in various cellular functions, including redox regulation and protection under oxidative stress. Despite the high number of Grx genes in plant genomes (48 Grxs in rice), the biological functions and physiological roles of m...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-06-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00740/full |
| _version_ | 1818560896918618112 |
|---|---|
| author | Pankaj Kumar Verma Shikha eVerma Veena ePande Shekhar eMallick Rudra Deo eTripathi Om Parkash eDhankher Debasis eChakrabarty |
| author_facet | Pankaj Kumar Verma Shikha eVerma Veena ePande Shekhar eMallick Rudra Deo eTripathi Om Parkash eDhankher Debasis eChakrabarty |
| author_sort | Pankaj Kumar Verma |
| collection | DOAJ |
| description | Glutaredoxins (Grxs) are a family of small multifunctional proteins involved in various cellular functions, including redox regulation and protection under oxidative stress. Despite the high number of Grx genes in plant genomes (48 Grxs in rice), the biological functions and physiological roles of most of them remain unknown. Here, the functional characterization of the two arsenic-responsive rice Grx family proteins, OsGrx_C7 and OsGrx_C2.1 are reported. Over-expression of OsGrx_C7 and OsGrx_C2.1 in transgenic Arabidopsis thaliana conferred arsenic (As) tolerance as reflected by germination, root growth assay, and whole plant growth. Also, the transgenic expression of OsGrxs displayed significantly reduced As accumulation in A. thaliana seeds and shoot tissues compared to WT plants during both AsIII and AsV stress. Thus, OsGrx_C7 and OsGrx_C2.1 seem to be an important determinant of As-stress response in plants. OsGrx_C7 and OsGrx_C2.1 transgenic showed to maintain intracellular GSH pool and involved in lowering AsIII accumulation either by extrusion or reducing uptake by altering the transcript of A. thaliana AtNIPs. Overall, OsGrx_C7 and OsGrx_C2.1 may represent a Grx family protein involved in As stress response and may allow a better understanding of the As induced stress pathways and the design of strategies for the improvement of stress tolerance as well as decreased As content in crops. |
| first_indexed | 2024-12-14T00:44:09Z |
| format | Article |
| id | doaj.art-a96a08e7ad5f4badaed98051307458c3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T00:44:09Z |
| publishDate | 2016-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a96a08e7ad5f4badaed98051307458c32022-12-21T23:24:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-06-01710.3389/fpls.2016.00740189726Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thalianaPankaj Kumar Verma0Shikha eVerma1Veena ePande2Shekhar eMallick3Rudra Deo eTripathi4Om Parkash eDhankher5Debasis eChakrabarty6National Botanical Research InstituteNational Botanical Research InstituteKumaun UniversityNational Botanical Research InstituteNational Botanical Research InstituteUniversity of MassachusettsNational Botanical Research InstituteGlutaredoxins (Grxs) are a family of small multifunctional proteins involved in various cellular functions, including redox regulation and protection under oxidative stress. Despite the high number of Grx genes in plant genomes (48 Grxs in rice), the biological functions and physiological roles of most of them remain unknown. Here, the functional characterization of the two arsenic-responsive rice Grx family proteins, OsGrx_C7 and OsGrx_C2.1 are reported. Over-expression of OsGrx_C7 and OsGrx_C2.1 in transgenic Arabidopsis thaliana conferred arsenic (As) tolerance as reflected by germination, root growth assay, and whole plant growth. Also, the transgenic expression of OsGrxs displayed significantly reduced As accumulation in A. thaliana seeds and shoot tissues compared to WT plants during both AsIII and AsV stress. Thus, OsGrx_C7 and OsGrx_C2.1 seem to be an important determinant of As-stress response in plants. OsGrx_C7 and OsGrx_C2.1 transgenic showed to maintain intracellular GSH pool and involved in lowering AsIII accumulation either by extrusion or reducing uptake by altering the transcript of A. thaliana AtNIPs. Overall, OsGrx_C7 and OsGrx_C2.1 may represent a Grx family protein involved in As stress response and may allow a better understanding of the As induced stress pathways and the design of strategies for the improvement of stress tolerance as well as decreased As content in crops.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00740/fullArsenicOryza sativaGSHglutaredoxinOsGrxs |
| spellingShingle | Pankaj Kumar Verma Shikha eVerma Veena ePande Shekhar eMallick Rudra Deo eTripathi Om Parkash eDhankher Debasis eChakrabarty Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana Frontiers in Plant Science Arsenic Oryza sativa GSH glutaredoxin OsGrxs |
| title | Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana |
| title_full | Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana |
| title_fullStr | Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana |
| title_full_unstemmed | Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana |
| title_short | Overexpression of rice glutaredoxin OsGrx_C7 and OsGrx_C2.1 reduces intracellular arsenic accumulation and increases tolerance in Arabidopsis thaliana |
| title_sort | overexpression of rice glutaredoxin osgrx c7 and osgrx c2 1 reduces intracellular arsenic accumulation and increases tolerance in arabidopsis thaliana |
| topic | Arsenic Oryza sativa GSH glutaredoxin OsGrxs |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00740/full |
| work_keys_str_mv | AT pankajkumarverma overexpressionofriceglutaredoxinosgrxc7andosgrxc21reducesintracellulararsenicaccumulationandincreasestoleranceinarabidopsisthaliana AT shikhaeverma overexpressionofriceglutaredoxinosgrxc7andosgrxc21reducesintracellulararsenicaccumulationandincreasestoleranceinarabidopsisthaliana AT veenaepande overexpressionofriceglutaredoxinosgrxc7andosgrxc21reducesintracellulararsenicaccumulationandincreasestoleranceinarabidopsisthaliana AT shekharemallick overexpressionofriceglutaredoxinosgrxc7andosgrxc21reducesintracellulararsenicaccumulationandincreasestoleranceinarabidopsisthaliana AT rudradeoetripathi overexpressionofriceglutaredoxinosgrxc7andosgrxc21reducesintracellulararsenicaccumulationandincreasestoleranceinarabidopsisthaliana AT omparkashedhankher overexpressionofriceglutaredoxinosgrxc7andosgrxc21reducesintracellulararsenicaccumulationandincreasestoleranceinarabidopsisthaliana AT debasisechakrabarty overexpressionofriceglutaredoxinosgrxc7andosgrxc21reducesintracellulararsenicaccumulationandincreasestoleranceinarabidopsisthaliana |