Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression
Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2006-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-1103 |
| _version_ | 1811290281700491264 |
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| author | Laurent Laquitaine Eric Gomès Julie François Chloé Marchive Stéphanie Pascal Saïd Hamdi Rossitza Atanassova Serge Delrot Pierre Coutos-Thévenot |
| author_facet | Laurent Laquitaine Eric Gomès Julie François Chloé Marchive Stéphanie Pascal Saïd Hamdi Rossitza Atanassova Serge Delrot Pierre Coutos-Thévenot |
| author_sort | Laurent Laquitaine |
| collection | DOAJ |
| description | Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-glucuronidase (GUS) reporter gene, and introduced in tobacco plants and grapevine cell suspensions using Agrobacterium spp. Single MYB- and the W-boxes identified on the 0.250-kbp fragment were sufficient to induce GUS activity in transgenic tobacco plants after transient expression of MYB and WRKY. Ergosterol, a nonspecific fungal elicitor, induced GUS activity in transgenic grapevine cell suspensions transformed with the 1,420- and 750-bp promoter containing a palindromic arrangement of two Wboxes but not the 650- or 250-bp fragment, where only one W-box was present. Moreover, ergosterol triggered WRKY, VvLTP1, and stilbene synthase gene expression in grape plantlets and enhanced protection against Botrytis cinerea. The molecular basis of ergosterol-induced protection is discussed. |
| first_indexed | 2024-04-13T04:10:10Z |
| format | Article |
| id | doaj.art-a96c541d3125404aa375c2851a5a5a8d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:10:10Z |
| publishDate | 2006-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-a96c541d3125404aa375c2851a5a5a8d2022-12-22T03:03:07ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062006-10-0119101103111210.1094/MPMI-19-1103Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene ExpressionLaurent LaquitaineEric GomèsJulie FrançoisChloé MarchiveStéphanie PascalSaïd HamdiRossitza AtanassovaSerge DelrotPierre Coutos-ThévenotType I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-glucuronidase (GUS) reporter gene, and introduced in tobacco plants and grapevine cell suspensions using Agrobacterium spp. Single MYB- and the W-boxes identified on the 0.250-kbp fragment were sufficient to induce GUS activity in transgenic tobacco plants after transient expression of MYB and WRKY. Ergosterol, a nonspecific fungal elicitor, induced GUS activity in transgenic grapevine cell suspensions transformed with the 1,420- and 750-bp promoter containing a palindromic arrangement of two Wboxes but not the 650- or 250-bp fragment, where only one W-box was present. Moreover, ergosterol triggered WRKY, VvLTP1, and stilbene synthase gene expression in grape plantlets and enhanced protection against Botrytis cinerea. The molecular basis of ergosterol-induced protection is discussed.https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-1103phytoalexin |
| spellingShingle | Laurent Laquitaine Eric Gomès Julie François Chloé Marchive Stéphanie Pascal Saïd Hamdi Rossitza Atanassova Serge Delrot Pierre Coutos-Thévenot Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression Molecular Plant-Microbe Interactions phytoalexin |
| title | Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression |
| title_full | Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression |
| title_fullStr | Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression |
| title_full_unstemmed | Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression |
| title_short | Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression |
| title_sort | molecular basis of ergosterol induced protection of grape against botrytis cinerea induction of type i ltp promoter activity wrky and stilbene synthase gene expression |
| topic | phytoalexin |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-19-1103 |
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