In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition
In recent years, several studies have validated the use of piezoelectric materials for in situ biological stimulation, opening new interesting insights for bio-electric therapies. In this work, we investigate the morphological properties of polyvinylidene fluoride (PVDF) in the form of microstructur...
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MDPI AG
2021-09-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/18/5232 |
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| author | Marco A. Alvarez-Perez Valentina Cirillo Maria Giovanna Pastore Carbone Marianna Pannico Pellegrino Musto Vincenzo Guarino |
| author_facet | Marco A. Alvarez-Perez Valentina Cirillo Maria Giovanna Pastore Carbone Marianna Pannico Pellegrino Musto Vincenzo Guarino |
| author_sort | Marco A. Alvarez-Perez |
| collection | DOAJ |
| description | In recent years, several studies have validated the use of piezoelectric materials for in situ biological stimulation, opening new interesting insights for bio-electric therapies. In this work, we investigate the morphological properties of polyvinylidene fluoride (PVDF) in the form of microstructured films after temperature-driven phase transition. The work aims to investigate the correlations between morphology at micrometric (i.e., spherulite size) and sub-micrometric (i.e., phase crystallinity) scale and in vitro cell response to validate their use as bio-functional interfaces for cellular studies. Morphological analyses (SEM, AFM) enabled evidence of the peculiar spherulite-like structure and the dependence of surface properties (i.e., intra-/interdomain roughness) upon process conditions (i.e., temperature). Meanwhile, chemical (i.e., FTIR) and thermal (i.e., DSC) analyses highlighted an influence of casting temperature and polymer solution on apolar to polar phases transition, thus affecting in vitro cell response. Accordingly, in vitro tests confirmed the relationship between micro/sub-microstructural properties and hMSC response in terms of adhesion and viability, thus suggesting a promising use of PVDF films to model, in perspective, in vitro functionalities of cells under electrical stimuli upon mechanical solicitation. |
| first_indexed | 2024-03-10T07:29:13Z |
| format | Article |
| id | doaj.art-a96efc3779b442dabe2738fdb0fc1a2e |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T07:29:13Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-a96efc3779b442dabe2738fdb0fc1a2e2023-11-22T14:00:54ZengMDPI AGMaterials1996-19442021-09-011418523210.3390/ma14185232In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase TransitionMarco A. Alvarez-Perez0Valentina Cirillo1Maria Giovanna Pastore Carbone2Marianna Pannico3Pellegrino Musto4Vincenzo Guarino5TBL-DEPeI, Universidad Nacional Autonoma de Mexico (UNAM), Mexico City 04510, MexicoInstitute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy, Mostra d’Oltremare Pad.20, Viale J.F. Kennedy 54, 80125 Naples, ItalyInstitute of Chemical Engineering Sciences, Foundation for Research, and Technology Hellas (FORTH-ICEHT), Stadiou St, Platani GR-26504, 26504 Patras, GreeceInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 32, 80078 Pozzuoli, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 32, 80078 Pozzuoli, ItalyInstitute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy, Mostra d’Oltremare Pad.20, Viale J.F. Kennedy 54, 80125 Naples, ItalyIn recent years, several studies have validated the use of piezoelectric materials for in situ biological stimulation, opening new interesting insights for bio-electric therapies. In this work, we investigate the morphological properties of polyvinylidene fluoride (PVDF) in the form of microstructured films after temperature-driven phase transition. The work aims to investigate the correlations between morphology at micrometric (i.e., spherulite size) and sub-micrometric (i.e., phase crystallinity) scale and in vitro cell response to validate their use as bio-functional interfaces for cellular studies. Morphological analyses (SEM, AFM) enabled evidence of the peculiar spherulite-like structure and the dependence of surface properties (i.e., intra-/interdomain roughness) upon process conditions (i.e., temperature). Meanwhile, chemical (i.e., FTIR) and thermal (i.e., DSC) analyses highlighted an influence of casting temperature and polymer solution on apolar to polar phases transition, thus affecting in vitro cell response. Accordingly, in vitro tests confirmed the relationship between micro/sub-microstructural properties and hMSC response in terms of adhesion and viability, thus suggesting a promising use of PVDF films to model, in perspective, in vitro functionalities of cells under electrical stimuli upon mechanical solicitation.https://www.mdpi.com/1996-1944/14/18/5232morphologyAFMin vitro responsehMSCpiezoelectric materials |
| spellingShingle | Marco A. Alvarez-Perez Valentina Cirillo Maria Giovanna Pastore Carbone Marianna Pannico Pellegrino Musto Vincenzo Guarino In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition Materials morphology AFM in vitro response hMSC piezoelectric materials |
| title | In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition |
| title_full | In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition |
| title_fullStr | In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition |
| title_full_unstemmed | In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition |
| title_short | In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition |
| title_sort | in vitro cell interactions on pvdf films effects of surface morphology and polar phase transition |
| topic | morphology AFM in vitro response hMSC piezoelectric materials |
| url | https://www.mdpi.com/1996-1944/14/18/5232 |
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