Cell Immobilization for Erythritol Production
Nowadays, commercial erythritol synthesis is performed by free-cell fermentation with fungi in liquid media containing high concentrations of pure carbon sources. Alternative fermentation techniques, such as cell immobilization, could imply an economic and energetic improvement for erythritol-produc...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/12/1286 |
| _version_ | 1827638195469156352 |
|---|---|
| author | María Hijosa-Valsero Ana I. Paniagua-García Rebeca Díez-Antolínez |
| author_facet | María Hijosa-Valsero Ana I. Paniagua-García Rebeca Díez-Antolínez |
| author_sort | María Hijosa-Valsero |
| collection | DOAJ |
| description | Nowadays, commercial erythritol synthesis is performed by free-cell fermentation with fungi in liquid media containing high concentrations of pure carbon sources. Alternative fermentation techniques, such as cell immobilization, could imply an economic and energetic improvement for erythritol-producing factories. The present work describes, for the first time, the feasibility of achieving cell immobilization during erythritol production. Cells of the fungus <i>Moniliella pollinis</i> were successfully immobilized on a cotton cloth which was placed inside a 2-L bioreactor, where they were fed with red grape must supplemented with yeast extract. They produced 47.03 ± 6.16 g/L erythritol in 96 h (yield 0.18 ± 0.04 g/g) over four consecutive fermentation batches. The immobilized cells remained stable and operative during a 456 h period. The erythritol concentration attained was similar (<i>p</i> > 0.05; Tukey HSD test) to the reference value obtained with the use of free cells (41.88 ± 5.18 g/L erythritol) under the same fermentation conditions. The comparable results observed for free and immobilized cells evidences the efficiency of the immobilization system. Therefore, the proposed method for erythritol bioproduction eliminates the need for the continuous preparation of fungal inocula before each fermentation batch, thus reducing the costs of the reagents and energy. |
| first_indexed | 2024-03-09T16:14:09Z |
| format | Article |
| id | doaj.art-a97189ba02004ca3a74fbe2febeb35fd |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T16:14:09Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-a97189ba02004ca3a74fbe2febeb35fd2023-11-24T15:59:53ZengMDPI AGJournal of Fungi2309-608X2022-12-01812128610.3390/jof8121286Cell Immobilization for Erythritol ProductionMaría Hijosa-Valsero0Ana I. Paniagua-García1Rebeca Díez-Antolínez2Centro de Innovación en Bioproductos Agroalimentarios (CIBAG), Instituto Tecnológico Agrario de Castilla y León (ITACyL), Polígono Agroindustrial del Órbigo p. 2–6, Villarejo de Órbigo, E-24358 León, SpainCentro de Innovación en Bioproductos Agroalimentarios (CIBAG), Instituto Tecnológico Agrario de Castilla y León (ITACyL), Polígono Agroindustrial del Órbigo p. 2–6, Villarejo de Órbigo, E-24358 León, SpainCentro de Innovación en Bioproductos Agroalimentarios (CIBAG), Instituto Tecnológico Agrario de Castilla y León (ITACyL), Polígono Agroindustrial del Órbigo p. 2–6, Villarejo de Órbigo, E-24358 León, SpainNowadays, commercial erythritol synthesis is performed by free-cell fermentation with fungi in liquid media containing high concentrations of pure carbon sources. Alternative fermentation techniques, such as cell immobilization, could imply an economic and energetic improvement for erythritol-producing factories. The present work describes, for the first time, the feasibility of achieving cell immobilization during erythritol production. Cells of the fungus <i>Moniliella pollinis</i> were successfully immobilized on a cotton cloth which was placed inside a 2-L bioreactor, where they were fed with red grape must supplemented with yeast extract. They produced 47.03 ± 6.16 g/L erythritol in 96 h (yield 0.18 ± 0.04 g/g) over four consecutive fermentation batches. The immobilized cells remained stable and operative during a 456 h period. The erythritol concentration attained was similar (<i>p</i> > 0.05; Tukey HSD test) to the reference value obtained with the use of free cells (41.88 ± 5.18 g/L erythritol) under the same fermentation conditions. The comparable results observed for free and immobilized cells evidences the efficiency of the immobilization system. Therefore, the proposed method for erythritol bioproduction eliminates the need for the continuous preparation of fungal inocula before each fermentation batch, thus reducing the costs of the reagents and energy.https://www.mdpi.com/2309-608X/8/12/1286immobilizationerythritolwinery surplusrepeated batchesfermentation |
| spellingShingle | María Hijosa-Valsero Ana I. Paniagua-García Rebeca Díez-Antolínez Cell Immobilization for Erythritol Production Journal of Fungi immobilization erythritol winery surplus repeated batches fermentation |
| title | Cell Immobilization for Erythritol Production |
| title_full | Cell Immobilization for Erythritol Production |
| title_fullStr | Cell Immobilization for Erythritol Production |
| title_full_unstemmed | Cell Immobilization for Erythritol Production |
| title_short | Cell Immobilization for Erythritol Production |
| title_sort | cell immobilization for erythritol production |
| topic | immobilization erythritol winery surplus repeated batches fermentation |
| url | https://www.mdpi.com/2309-608X/8/12/1286 |
| work_keys_str_mv | AT mariahijosavalsero cellimmobilizationforerythritolproduction AT anaipaniaguagarcia cellimmobilizationforerythritolproduction AT rebecadiezantolinez cellimmobilizationforerythritolproduction |