Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation
Anhydrobiotes are rare microbes, plants and animals that tolerate severe water loss. Understanding the molecular basis for their desiccation tolerance may provide novel insights into stress biology and critical tools for engineering drought-tolerant crops. Using the anhydrobiote, budding yeast, we s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/38337 |
| _version_ | 1811181113448595456 |
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| author | Skylar Xantus Kim Gamze Çamdere Xuchen Hu Douglas Koshland Hugo Tapia |
| author_facet | Skylar Xantus Kim Gamze Çamdere Xuchen Hu Douglas Koshland Hugo Tapia |
| author_sort | Skylar Xantus Kim |
| collection | DOAJ |
| description | Anhydrobiotes are rare microbes, plants and animals that tolerate severe water loss. Understanding the molecular basis for their desiccation tolerance may provide novel insights into stress biology and critical tools for engineering drought-tolerant crops. Using the anhydrobiote, budding yeast, we show that trehalose and Hsp12, a small intrinsically disordered protein (sIDP) of the hydrophilin family, synergize to mitigate completely the inviability caused by the lethal stresses of desiccation. We show that these two molecules help to stabilize the activity and prevent aggregation of model proteins both in vivo and in vitro. We also identify a novel in vitro role for Hsp12 as a membrane remodeler, a protective feature not shared by another yeast hydrophilin, suggesting that sIDPs have distinct biological functions. |
| first_indexed | 2024-04-11T09:14:11Z |
| format | Article |
| id | doaj.art-a9746c5fba25405ca66f791a7061e66b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:14:11Z |
| publishDate | 2018-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a9746c5fba25405ca66f791a7061e66b2022-12-22T04:32:25ZengeLife Sciences Publications LtdeLife2050-084X2018-07-01710.7554/eLife.38337Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccationSkylar Xantus Kim0Gamze Çamdere1Xuchen Hu2Douglas Koshland3https://orcid.org/0000-0003-3742-6294Hugo Tapia4https://orcid.org/0000-0003-1901-2151Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesAnhydrobiotes are rare microbes, plants and animals that tolerate severe water loss. Understanding the molecular basis for their desiccation tolerance may provide novel insights into stress biology and critical tools for engineering drought-tolerant crops. Using the anhydrobiote, budding yeast, we show that trehalose and Hsp12, a small intrinsically disordered protein (sIDP) of the hydrophilin family, synergize to mitigate completely the inviability caused by the lethal stresses of desiccation. We show that these two molecules help to stabilize the activity and prevent aggregation of model proteins both in vivo and in vitro. We also identify a novel in vitro role for Hsp12 as a membrane remodeler, a protective feature not shared by another yeast hydrophilin, suggesting that sIDPs have distinct biological functions.https://elifesciences.org/articles/38337desiccation tolerancetrehalosesIDPHsp12 |
| spellingShingle | Skylar Xantus Kim Gamze Çamdere Xuchen Hu Douglas Koshland Hugo Tapia Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation eLife desiccation tolerance trehalose sIDP Hsp12 |
| title | Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation |
| title_full | Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation |
| title_fullStr | Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation |
| title_full_unstemmed | Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation |
| title_short | Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation |
| title_sort | synergy between the small intrinsically disordered protein hsp12 and trehalose sustain viability after severe desiccation |
| topic | desiccation tolerance trehalose sIDP Hsp12 |
| url | https://elifesciences.org/articles/38337 |
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