Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing
ABSTRACTThe aim of this study was to reveal the mechanism of its wound healing function at the molecular level using transcriptome sequencing technology. The results showed that EAE mainly upregulated Cyclin A gene through AMPK signalling pathway, activated cell cycle pathway, promoted DNA synthesis...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/09540105.2023.2214709 |
| _version_ | 1797447867249459200 |
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| author | Yongling Wu Chang Liu Wenjing Lu Siting Li Zhou Chen Junxia Xia Yiling Tian Aijin Ma Yingmin Jia |
| author_facet | Yongling Wu Chang Liu Wenjing Lu Siting Li Zhou Chen Junxia Xia Yiling Tian Aijin Ma Yingmin Jia |
| author_sort | Yongling Wu |
| collection | DOAJ |
| description | ABSTRACTThe aim of this study was to reveal the mechanism of its wound healing function at the molecular level using transcriptome sequencing technology. The results showed that EAE mainly upregulated Cyclin A gene through AMPK signalling pathway, activated cell cycle pathway, promoted DNA synthesis, accelerated mitosis, and thus promoted the proliferation of Human immortalized keratinocytes (HaCaT) cells. In addition, analysis of downregulated gene enrichment revealed that these genes were significantly or very significantly enriched not only in the cytokine-cytokine receptor interaction pathway but also in the TNF and the NF-κB signalling inflammatory classical pathway. It was mainly through downregulation of genes such as Fn14, CCL2, CCL5, CARD14 and LTB that prevented inflammatory responses while delaying apoptosis. The results of this study provided important information for the molecular mechanism of wound healing of EAE and provided a basis for further development of functional products of raspberry. |
| first_indexed | 2024-03-09T14:02:10Z |
| format | Article |
| id | doaj.art-a975ea16a4654cdc8e127d683f4ddc96 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-03-09T14:02:10Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-a975ea16a4654cdc8e127d683f4ddc962023-11-30T06:57:34ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432023-12-0134110.1080/09540105.2023.2214709Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healingYongling Wu0Chang Liu1Wenjing Lu2Siting Li3Zhou Chen4Junxia Xia5Yiling Tian6Aijin Ma7Yingmin Jia8School of Food and Health, Beijing Technology and Business University, Beijing, People’s Republic of ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing, People’s Republic of ChinaBeijing Institute of Biological Products Ltd., Beijing, People’s Republic of ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing, People’s Republic of ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing, People’s Republic of ChinaHebei Yangyuan ZhiHui Beverage Co., Ltd., Hengshui, HebeiCollege of Food Science and Technology, Hebei Agricultural University, Baoding, HebeiSchool of Food and Health, Beijing Technology and Business University, Beijing, People’s Republic of ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing, People’s Republic of ChinaABSTRACTThe aim of this study was to reveal the mechanism of its wound healing function at the molecular level using transcriptome sequencing technology. The results showed that EAE mainly upregulated Cyclin A gene through AMPK signalling pathway, activated cell cycle pathway, promoted DNA synthesis, accelerated mitosis, and thus promoted the proliferation of Human immortalized keratinocytes (HaCaT) cells. In addition, analysis of downregulated gene enrichment revealed that these genes were significantly or very significantly enriched not only in the cytokine-cytokine receptor interaction pathway but also in the TNF and the NF-κB signalling inflammatory classical pathway. It was mainly through downregulation of genes such as Fn14, CCL2, CCL5, CARD14 and LTB that prevented inflammatory responses while delaying apoptosis. The results of this study provided important information for the molecular mechanism of wound healing of EAE and provided a basis for further development of functional products of raspberry.https://www.tandfonline.com/doi/10.1080/09540105.2023.2214709Raspberryellagic acidcell proliferationtranscriptomewound healing |
| spellingShingle | Yongling Wu Chang Liu Wenjing Lu Siting Li Zhou Chen Junxia Xia Yiling Tian Aijin Ma Yingmin Jia Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing Food and Agricultural Immunology Raspberry ellagic acid cell proliferation transcriptome wound healing |
| title | Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing |
| title_full | Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing |
| title_fullStr | Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing |
| title_full_unstemmed | Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing |
| title_short | Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing |
| title_sort | transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing |
| topic | Raspberry ellagic acid cell proliferation transcriptome wound healing |
| url | https://www.tandfonline.com/doi/10.1080/09540105.2023.2214709 |
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