Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi
he culture media commonly used for isolation are imported and expensive. Many organic materials are naturally decomposed from complex organic compounds to simple ones by microbes. Based on this principles, this study wants to make isolation media with ripe coconut waste-water as based substance, so...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Padjajaran
2011-09-01
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| Series: | Majalah Kedokteran Bandung |
| Subjects: | |
| Online Access: | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/56 |
| _version_ | 1811261450091495424 |
|---|---|
| author | Hanna Yolanda Yanti Mulyana |
| author_facet | Hanna Yolanda Yanti Mulyana |
| author_sort | Hanna Yolanda |
| collection | DOAJ |
| description | he culture media commonly used for isolation are imported and expensive. Many organic materials are naturally decomposed from complex organic compounds to simple ones by microbes. Based on this principles, this study wants to make isolation media with ripe coconut waste-water as based substance, so it can be considered as economical culture media. The method was laboratoric experimental by isolating tested bacteria with ripe coconut waste-water as based substance. The composition of the media were adjusted with MacConkey agar and blood agar base. Standard media were MacConkey agar and blood agar base. Control media were agar 15 g/L media and ripe coconut waste-water agar media. Tested bacteria were a number species of Enterobacteriaceae and positive gram cocci. The evaluated variables were macroscopic and microscopic images. Data was analized by Wilcoxon matched pairs test and sign test methods. This study did not find a significant differences (p>0.05) between standard media and ripe coconut waste-water media. Specific characteristics of tested bacteria, such as red colonies, mucoid, and hemolitic zone, were similar between standard media and ripe coconut waste-water media. The conclusion is ripe coconut waste-water can be used as base for isolation media substance of Enterobacteriaceae and gram positive cocci. |
| first_indexed | 2024-04-12T19:04:37Z |
| format | Article |
| id | doaj.art-a97aba3e655442f481912e1b839d5eb4 |
| institution | Directory Open Access Journal |
| issn | 0126-074X 2338-6223 |
| language | English |
| last_indexed | 2024-04-12T19:04:37Z |
| publishDate | 2011-09-01 |
| publisher | Universitas Padjajaran |
| record_format | Article |
| series | Majalah Kedokteran Bandung |
| spelling | doaj.art-a97aba3e655442f481912e1b839d5eb42022-12-22T03:20:03ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232011-09-0143311712110.15395/mkb.v43n3.56Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media IsolasiHanna Yolanda0Yanti Mulyana1Departemen Parasitologi Unika Atma JayDepartemen Mikrobiologi Fakultas Kedokteran Universitas Padjadjaran, Bandunghe culture media commonly used for isolation are imported and expensive. Many organic materials are naturally decomposed from complex organic compounds to simple ones by microbes. Based on this principles, this study wants to make isolation media with ripe coconut waste-water as based substance, so it can be considered as economical culture media. The method was laboratoric experimental by isolating tested bacteria with ripe coconut waste-water as based substance. The composition of the media were adjusted with MacConkey agar and blood agar base. Standard media were MacConkey agar and blood agar base. Control media were agar 15 g/L media and ripe coconut waste-water agar media. Tested bacteria were a number species of Enterobacteriaceae and positive gram cocci. The evaluated variables were macroscopic and microscopic images. Data was analized by Wilcoxon matched pairs test and sign test methods. This study did not find a significant differences (p>0.05) between standard media and ripe coconut waste-water media. Specific characteristics of tested bacteria, such as red colonies, mucoid, and hemolitic zone, were similar between standard media and ripe coconut waste-water media. The conclusion is ripe coconut waste-water can be used as base for isolation media substance of Enterobacteriaceae and gram positive cocci.http://journal.fk.unpad.ac.id/index.php/mkb/article/view/56Blood agar baseMacConkey agarisolationmediaripe coconut waste-water |
| spellingShingle | Hanna Yolanda Yanti Mulyana Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi Majalah Kedokteran Bandung Blood agar base MacConkey agar isolation media ripe coconut waste-water |
| title | Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi |
| title_full | Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi |
| title_fullStr | Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi |
| title_full_unstemmed | Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi |
| title_short | Uji Coba Penggunaan Limbah Air Kelapa Tua sebagai Bahan Dasar Media Isolasi |
| title_sort | uji coba penggunaan limbah air kelapa tua sebagai bahan dasar media isolasi |
| topic | Blood agar base MacConkey agar isolation media ripe coconut waste-water |
| url | http://journal.fk.unpad.ac.id/index.php/mkb/article/view/56 |
| work_keys_str_mv | AT hannayolanda ujicobapenggunaanlimbahairkelapatuasebagaibahandasarmediaisolasi AT yantimulyana ujicobapenggunaanlimbahairkelapatuasebagaibahandasarmediaisolasi |