Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences
Abstract Background Anaplasma ovis (A. ovis) is the predominant causative agent of anaplasmosis in goats and sheep in most tropical and subtropical regions of the world. However, there is considerable variation in reported infection rates, breed susceptibility, and controversial findings regarding t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.1404 |
| _version_ | 1797263345119657984 |
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| author | Maryam Abbas Zadeh Hossein Hamidinejat Ali Abbas Nikvand Seyedeh Missagh Jalali Somayeh Bahrami |
| author_facet | Maryam Abbas Zadeh Hossein Hamidinejat Ali Abbas Nikvand Seyedeh Missagh Jalali Somayeh Bahrami |
| author_sort | Maryam Abbas Zadeh |
| collection | DOAJ |
| description | Abstract Background Anaplasma ovis (A. ovis) is the predominant causative agent of anaplasmosis in goats and sheep in most tropical and subtropical regions of the world. However, there is considerable variation in reported infection rates, breed susceptibility, and controversial findings regarding the haemolytic effects of A. ovis infection in goats. Objectives Thus, we investigated the molecular and haematological aspects of A. ovis infection in goats from Ahvaz city. Methods One hundred and fifty apparently healthy goats (74 blacks and 76 Najdi goats) were randomly sampled from six flocks in the Ahvaz suburb during ticks’ activity season. Haematological evaluation, smear microscopic (SM) examination and PCR assay were performed to assess A. ovis infection. Additionally, the percentage of parasitemia was determined from blood smears. Results SM examination revealed that 25.7% of the goats displayed erythrocyte Anaplasma‐like inclusion bodies. PCR analysis indicated that 54% of the goats were positive for A. ovis infection (44.6% of blacks and 63.2% of Najdi goats). No significant difference in haematological values was observed between healthy and infected goats based on PCR testing. However, a significant difference in haematological indices was observed between the group with parasitemia level of 0.01–0.02% (SM and PCR positive) compared to the healthy goats (SM and PCR negative), particularly concerning Hb, PCV and RBC count (p < 0.01). Conclusions When the parasitemia exceeds 0.01%, A. ovis infection may disrupt haematological parameters in infected goats. The high prevalence of A. ovis infection (54%) among the studied goats underscores the importance of giving special attention to implementing necessary measures for disease control in the Ahvaz suburb. |
| first_indexed | 2024-04-25T00:11:32Z |
| format | Article |
| id | doaj.art-a97b5f04f08147b8b2dd50c068501612 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-25T00:11:32Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-a97b5f04f08147b8b2dd50c0685016122024-03-13T13:16:36ZengWileyVeterinary Medicine and Science2053-10952024-03-01102n/an/a10.1002/vms3.1404Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influencesMaryam Abbas Zadeh0Hossein Hamidinejat1Ali Abbas Nikvand2Seyedeh Missagh Jalali3Somayeh Bahrami4Faculty of Veterinary MedicineDepartment of PathobiologyShahid Chamran University of AhvazAhvaz IranFaculty of Veterinary Medicine Department of Pathobiology Shahid Chamran University of Ahvaz Ahvaz IranFaculty of Veterinary Medicine Department of Clinical Sciences Shahid Chamran University of Ahvaz Ahvaz IranFaculty of Veterinary Medicine Department of Clinical Sciences Shahid Chamran University of Ahvaz Ahvaz IranFaculty of Veterinary Medicine Department of Pathobiology Shahid Chamran University of Ahvaz Ahvaz IranAbstract Background Anaplasma ovis (A. ovis) is the predominant causative agent of anaplasmosis in goats and sheep in most tropical and subtropical regions of the world. However, there is considerable variation in reported infection rates, breed susceptibility, and controversial findings regarding the haemolytic effects of A. ovis infection in goats. Objectives Thus, we investigated the molecular and haematological aspects of A. ovis infection in goats from Ahvaz city. Methods One hundred and fifty apparently healthy goats (74 blacks and 76 Najdi goats) were randomly sampled from six flocks in the Ahvaz suburb during ticks’ activity season. Haematological evaluation, smear microscopic (SM) examination and PCR assay were performed to assess A. ovis infection. Additionally, the percentage of parasitemia was determined from blood smears. Results SM examination revealed that 25.7% of the goats displayed erythrocyte Anaplasma‐like inclusion bodies. PCR analysis indicated that 54% of the goats were positive for A. ovis infection (44.6% of blacks and 63.2% of Najdi goats). No significant difference in haematological values was observed between healthy and infected goats based on PCR testing. However, a significant difference in haematological indices was observed between the group with parasitemia level of 0.01–0.02% (SM and PCR positive) compared to the healthy goats (SM and PCR negative), particularly concerning Hb, PCV and RBC count (p < 0.01). Conclusions When the parasitemia exceeds 0.01%, A. ovis infection may disrupt haematological parameters in infected goats. The high prevalence of A. ovis infection (54%) among the studied goats underscores the importance of giving special attention to implementing necessary measures for disease control in the Ahvaz suburb.https://doi.org/10.1002/vms3.1404Anaplasma ovisNajdi goatparasitemiaPCRsmear microscopy |
| spellingShingle | Maryam Abbas Zadeh Hossein Hamidinejat Ali Abbas Nikvand Seyedeh Missagh Jalali Somayeh Bahrami Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences Veterinary Medicine and Science Anaplasma ovis Najdi goat parasitemia PCR smear microscopy |
| title | Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences |
| title_full | Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences |
| title_fullStr | Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences |
| title_full_unstemmed | Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences |
| title_short | Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences |
| title_sort | molecular and haematological investigations on anaplasma ovis infection in goats in ahvaz iran insights into infection rate haemolytic effect and breed influences |
| topic | Anaplasma ovis Najdi goat parasitemia PCR smear microscopy |
| url | https://doi.org/10.1002/vms3.1404 |
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