Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system
Abstract Background 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, i...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | http://link.springer.com/article/10.1186/s13068-017-0886-x |
| _version_ | 1828825232164519936 |
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| author | Changman Kim Mi Yeon Kim Iain Michie Byong-Hun Jeon Giuliano C. Premier Sunghoon Park Jung Rae Kim |
| author_facet | Changman Kim Mi Yeon Kim Iain Michie Byong-Hun Jeon Giuliano C. Premier Sunghoon Park Jung Rae Kim |
| author_sort | Changman Kim |
| collection | DOAJ |
| description | Abstract Background 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD+ under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Results In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase (AldH) was developed and cultured in a bioelectrochemical system (BES) with the application of an electrical potential to the anode using a chronoamperometric method (+0.5 V vs. Ag/AgCl). The BES operation resulted in 1.7-fold enhancement of 3-HP production compared to the control without the applied potential. The intracellular NADH/NAD+ ratio was significantly lower when the L17 cells were grown under an electric potential. The interaction between the electrode and overexpressed AldH was enhanced by electron shuttling mediated by HNQ (2-hydroxy-1,4-naphthoquinone). Conclusions Enhanced 3-HP production by the BES was achieved using recombinant K. pneumoniae L17. The quinone-based electron transference between the electrode and L17 was investigated by respiratory uncoupler experiments. This study provides a novel strategy to control the intracellular redox states to enhance the yield and titer of 3-HP production as well as other bioconversion processes. |
| first_indexed | 2024-12-12T14:13:22Z |
| format | Article |
| id | doaj.art-a97e60099b074a56aee158ad3cbb7b2e |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T14:13:22Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-a97e60099b074a56aee158ad3cbb7b2e2022-12-22T00:21:59ZengBMCBiotechnology for Biofuels1754-68342017-08-0110111110.1186/s13068-017-0886-xAnodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical systemChangman Kim0Mi Yeon Kim1Iain Michie2Byong-Hun Jeon3Giuliano C. Premier4Sunghoon Park5Jung Rae Kim6School of Chemical and Biomolecular Engineering, Pusan National UniversitySchool of Chemical and Biomolecular Engineering, Pusan National UniversitySustainable Environment Research Centre (SERC), Faculty of Computing, Engineering and Science, University of South WalesDepartment of Natural Resources and Environmental Engineering, Hanyang UniversitySustainable Environment Research Centre (SERC), Faculty of Computing, Engineering and Science, University of South WalesSchool of Chemical and Biomolecular Engineering, Pusan National UniversitySchool of Chemical and Biomolecular Engineering, Pusan National UniversityAbstract Background 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD+ under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Results In this study, recombinant K. pneumoniae L17 overexpressing aldehyde dehydrogenase (AldH) was developed and cultured in a bioelectrochemical system (BES) with the application of an electrical potential to the anode using a chronoamperometric method (+0.5 V vs. Ag/AgCl). The BES operation resulted in 1.7-fold enhancement of 3-HP production compared to the control without the applied potential. The intracellular NADH/NAD+ ratio was significantly lower when the L17 cells were grown under an electric potential. The interaction between the electrode and overexpressed AldH was enhanced by electron shuttling mediated by HNQ (2-hydroxy-1,4-naphthoquinone). Conclusions Enhanced 3-HP production by the BES was achieved using recombinant K. pneumoniae L17. The quinone-based electron transference between the electrode and L17 was investigated by respiratory uncoupler experiments. This study provides a novel strategy to control the intracellular redox states to enhance the yield and titer of 3-HP production as well as other bioconversion processes.http://link.springer.com/article/10.1186/s13068-017-0886-x3-hydroxypropionic acidElectro-fermentationBioelectrochemical systemKlebsiella pneumoniae L17 |
| spellingShingle | Changman Kim Mi Yeon Kim Iain Michie Byong-Hun Jeon Giuliano C. Premier Sunghoon Park Jung Rae Kim Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system Biotechnology for Biofuels 3-hydroxypropionic acid Electro-fermentation Bioelectrochemical system Klebsiella pneumoniae L17 |
| title | Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system |
| title_full | Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system |
| title_fullStr | Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system |
| title_full_unstemmed | Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system |
| title_short | Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system |
| title_sort | anodic electro fermentation of 3 hydroxypropionic acid from glycerol by recombinant klebsiella pneumoniae l17 in a bioelectrochemical system |
| topic | 3-hydroxypropionic acid Electro-fermentation Bioelectrochemical system Klebsiella pneumoniae L17 |
| url | http://link.springer.com/article/10.1186/s13068-017-0886-x |
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