Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
Background: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/11/5/1331 |
| _version_ | 1797600945401495552 |
|---|---|
| author | Ravil Garifulin Maria Davleeva Andrei Izmailov Filip Fadeev Vage Markosyan Roman Shevchenko Irina Minyazeva Tagir Minekayev Igor Lavrov Rustem Islamov |
| author_facet | Ravil Garifulin Maria Davleeva Andrei Izmailov Filip Fadeev Vage Markosyan Roman Shevchenko Irina Minyazeva Tagir Minekayev Igor Lavrov Rustem Islamov |
| author_sort | Ravil Garifulin |
| collection | DOAJ |
| description | Background: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in relation to SCI: distant changes in the spinal cord, peripheral nerve, and muscles. Methods: The changes in the spinal cord, the tibial nerve, and the hind limb muscles were evaluated in control SCI animals and after intravenous infusion of autologous leucoconcentrate enriched with genes encoding neuroprotective factors (VEGF, GDNF, and NCAM), which previously demonstrated a positive effect on post-traumatic restoration. Results: Two months after thoracic contusion in the treated mini pigs, a positive remodeling of the macro- and microglial cells, expression of PSD95 and Chat in the lumbar spinal cord, and preservation of the number and morphological characteristics of the myelinated fibers in the tibial nerve were observed and were aligned with hind limb motor recovery and reduced soleus muscle atrophy. Conclusion: Here, we show the positive effect of autologous genetically enriched leucoconcentrate-producing recombinant neuroprotective factors on targets distant to the primary lesion site in mini pigs with SCI. These findings open new perspectives for the therapy of SCI. |
| first_indexed | 2024-03-11T03:54:59Z |
| format | Article |
| id | doaj.art-a97e62b4fe9640779c81b4a51873a698 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T03:54:59Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-a97e62b4fe9640779c81b4a51873a6982023-11-18T00:35:27ZengMDPI AGBiomedicines2227-90592023-04-01115133110.3390/biomedicines11051331Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion InjuryRavil Garifulin0Maria Davleeva1Andrei Izmailov2Filip Fadeev3Vage Markosyan4Roman Shevchenko5Irina Minyazeva6Tagir Minekayev7Igor Lavrov8Rustem Islamov9Department of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaDepartment of Neurology, Mayo Clinic, Rochester, MN 55905, USADepartment of Histology, Cytology and Embryology, Kazan State Medical University, 420012 Kazan, RussiaBackground: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in relation to SCI: distant changes in the spinal cord, peripheral nerve, and muscles. Methods: The changes in the spinal cord, the tibial nerve, and the hind limb muscles were evaluated in control SCI animals and after intravenous infusion of autologous leucoconcentrate enriched with genes encoding neuroprotective factors (VEGF, GDNF, and NCAM), which previously demonstrated a positive effect on post-traumatic restoration. Results: Two months after thoracic contusion in the treated mini pigs, a positive remodeling of the macro- and microglial cells, expression of PSD95 and Chat in the lumbar spinal cord, and preservation of the number and morphological characteristics of the myelinated fibers in the tibial nerve were observed and were aligned with hind limb motor recovery and reduced soleus muscle atrophy. Conclusion: Here, we show the positive effect of autologous genetically enriched leucoconcentrate-producing recombinant neuroprotective factors on targets distant to the primary lesion site in mini pigs with SCI. These findings open new perspectives for the therapy of SCI.https://www.mdpi.com/2227-9059/11/5/1331spinal cord injuriesswineautologous blood transfusionleukocytesgenetic vectorsgenetic therapy |
| spellingShingle | Ravil Garifulin Maria Davleeva Andrei Izmailov Filip Fadeev Vage Markosyan Roman Shevchenko Irina Minyazeva Tagir Minekayev Igor Lavrov Rustem Islamov Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury Biomedicines spinal cord injuries swine autologous blood transfusion leukocytes genetic vectors genetic therapy |
| title | Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury |
| title_full | Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury |
| title_fullStr | Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury |
| title_full_unstemmed | Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury |
| title_short | Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury |
| title_sort | evaluation of the autologous genetically enriched leucoconcentrate on the lumbar spinal cord morpho functional recovery in a mini pig with thoracic spine contusion injury |
| topic | spinal cord injuries swine autologous blood transfusion leukocytes genetic vectors genetic therapy |
| url | https://www.mdpi.com/2227-9059/11/5/1331 |
| work_keys_str_mv | AT ravilgarifulin evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT mariadavleeva evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT andreiizmailov evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT filipfadeev evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT vagemarkosyan evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT romanshevchenko evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT irinaminyazeva evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT tagirminekayev evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT igorlavrov evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury AT rustemislamov evaluationoftheautologousgeneticallyenrichedleucoconcentrateonthelumbarspinalcordmorphofunctionalrecoveryinaminipigwiththoracicspinecontusioninjury |