Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47
ObjectiveThe low detection rate of early-stage and small tumors remains a clinical challenge. A solution to this unmet need is urgently warranted for the accurate diagnosis and treatment of bladder cancer (BC). This study aimed to evaluate the feasibility of CD47 as a target for optical molecular im...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1083553/full |
| _version_ | 1811162684258779136 |
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| author | Haifeng Hao Xinyu Wang Yan Qin Zhifang Ma Pengyu Yan Chao Liu Guanying Chen Xiaofeng Yang |
| author_facet | Haifeng Hao Xinyu Wang Yan Qin Zhifang Ma Pengyu Yan Chao Liu Guanying Chen Xiaofeng Yang |
| author_sort | Haifeng Hao |
| collection | DOAJ |
| description | ObjectiveThe low detection rate of early-stage and small tumors remains a clinical challenge. A solution to this unmet need is urgently warranted for the accurate diagnosis and treatment of bladder cancer (BC). This study aimed to evaluate the feasibility of CD47 as a target for optical molecular imaging of human BC and conduct preliminary ex vivo imaging experiments.MethodUsing indocyanine green (ICG) and a CD47 antibody (anti-CD47), we synthesized a new targeted fluorescent probe ICG-anti-CD47. A total of 25 patients undergoing radical cystectomy were prospectively included in ex vivo imaging experiments. Following surgery, the freshly isolated bladder specimens were incubated with ICG-anti-CD47, and images were captured under white light and near-infrared (NIR) light. Standard histopathologic evaluation was performed, and findings were correlated with those of CD47-targeted NIR molecular imaging.ResultsBased on the ex vivo imaging experiments, 23 and 2 patients were pathologically diagnosed with bladder urothelial carcinoma and bladder squamous cell carcinoma, respectively. There were no adverse effects of ICG-anti-CD47 on the histological structure of the tumor and normal uroepithelium. In the NIR grayscale images, the mean fluorescence intensity of the tumor tissue was significantly higher than that of the adjacent normal background tissue, which markedly improved tumor visualization.ConclusionAnti-CD47-targeted NIR molecular imaging may be a feasible and powerful strategy for the accurate diagnosis of BC. Nevertheless, larger-scale randomized trials are warranted to verify the present findings. |
| first_indexed | 2024-04-10T06:33:13Z |
| format | Article |
| id | doaj.art-a97f2b2907ea42c9948b79206cd99f3c |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T06:33:13Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-a97f2b2907ea42c9948b79206cd99f3c2023-03-01T05:17:51ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-03-011310.3389/fonc.2023.10835531083553Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47Haifeng Hao0Xinyu Wang1Yan Qin2Zhifang Ma3Pengyu Yan4Chao Liu5Guanying Chen6Xiaofeng Yang7Department of Urology, First Hospital of Shanxi Medical University, Taiyuan, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, ChinaDepartment of Gastroenterology, Shanxi Provincial People’s Hospital, Taiyuan, ChinaDepartment of Urology, First Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Urology, First Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Urology, First Hospital of Shanxi Medical University, Taiyuan, ChinaSchool of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, ChinaDepartment of Urology, First Hospital of Shanxi Medical University, Taiyuan, ChinaObjectiveThe low detection rate of early-stage and small tumors remains a clinical challenge. A solution to this unmet need is urgently warranted for the accurate diagnosis and treatment of bladder cancer (BC). This study aimed to evaluate the feasibility of CD47 as a target for optical molecular imaging of human BC and conduct preliminary ex vivo imaging experiments.MethodUsing indocyanine green (ICG) and a CD47 antibody (anti-CD47), we synthesized a new targeted fluorescent probe ICG-anti-CD47. A total of 25 patients undergoing radical cystectomy were prospectively included in ex vivo imaging experiments. Following surgery, the freshly isolated bladder specimens were incubated with ICG-anti-CD47, and images were captured under white light and near-infrared (NIR) light. Standard histopathologic evaluation was performed, and findings were correlated with those of CD47-targeted NIR molecular imaging.ResultsBased on the ex vivo imaging experiments, 23 and 2 patients were pathologically diagnosed with bladder urothelial carcinoma and bladder squamous cell carcinoma, respectively. There were no adverse effects of ICG-anti-CD47 on the histological structure of the tumor and normal uroepithelium. In the NIR grayscale images, the mean fluorescence intensity of the tumor tissue was significantly higher than that of the adjacent normal background tissue, which markedly improved tumor visualization.ConclusionAnti-CD47-targeted NIR molecular imaging may be a feasible and powerful strategy for the accurate diagnosis of BC. Nevertheless, larger-scale randomized trials are warranted to verify the present findings.https://www.frontiersin.org/articles/10.3389/fonc.2023.1083553/fullbladder carcinomaindocyanine greenCD47near-infrared fluorescencemolecular imaging |
| spellingShingle | Haifeng Hao Xinyu Wang Yan Qin Zhifang Ma Pengyu Yan Chao Liu Guanying Chen Xiaofeng Yang Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47 Frontiers in Oncology bladder carcinoma indocyanine green CD47 near-infrared fluorescence molecular imaging |
| title | Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47 |
| title_full | Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47 |
| title_fullStr | Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47 |
| title_full_unstemmed | Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47 |
| title_short | Ex vivo near-infrared targeted imaging of human bladder carcinoma by ICG-anti-CD47 |
| title_sort | ex vivo near infrared targeted imaging of human bladder carcinoma by icg anti cd47 |
| topic | bladder carcinoma indocyanine green CD47 near-infrared fluorescence molecular imaging |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1083553/full |
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