The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms
<i>Staphylococcus aureus</i> is one of the most important pathogens causing intramammary infection (IMI) in dairy herds. The goals of this study were (1) to describe the prevalence of <i>S. aureus</i> in Israeli dairy farms; (2) to characterize the <i>spa</i>-base...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/9/7/371 |
| _version_ | 1797433060856168448 |
|---|---|
| author | Adin Shwimmer Rama Falk Tamar K. De-Levie Michal Lasnoy Katia Levytskyi Amos Adler |
| author_facet | Adin Shwimmer Rama Falk Tamar K. De-Levie Michal Lasnoy Katia Levytskyi Amos Adler |
| author_sort | Adin Shwimmer |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> is one of the most important pathogens causing intramammary infection (IMI) in dairy herds. The goals of this study were (1) to describe the prevalence of <i>S. aureus</i> in Israeli dairy farms; (2) to characterize the <i>spa</i>-based clonal structure of mastitis-related <i>S. aureus</i> isolates; (3) to analyze the transmission network of a large outbreak within a single farm and (4) to characterize the virulence factors of the outbreak strain. The prevalence and the molecular survey were performed on all Israeli IMI-related isolates, 9.2019–8.2020. Molecular methods included <i>spa</i>-typing for the survey and whole-genome sequencing (WGS) for the investigation of the farm ‘A’ outbreak. During the one-year survey, <i>S. aureus</i> was identified in 152 dairy farms, with a total of 440 positive samples. The <i>spa</i> t2873 was found in 284 isolates (64.5%) across 112 farms (73.6%). Other common types included t529 (n = 46), t9303 (n = 34) and the methicillin-resistant <i>S. aureus</i> t011 (n = 11). The highest number of cases (n = 25) was detected in Farm ‘A’, all of which were found to be <i>spa</i> t2873. Phylogenetic analysis confirmed that most transmission events occurred within the same milking group, and inter-group transmission was due to the transfer of cows between groups or due to consecutive milking order. The <i>spa</i> t2873 strain contained putative virulence genes, including various intracellular and collagen adhesion proteins. Our study revealed the dissemination of the t2873 strain to the majority of Israeli dairy farms. The possibility of inter-farm transmission should be monitored and prevented. |
| first_indexed | 2024-03-09T10:10:40Z |
| format | Article |
| id | doaj.art-a9804a04bcc742bc9142bbf34661120a |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T10:10:40Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-a9804a04bcc742bc9142bbf34661120a2023-12-01T22:47:56ZengMDPI AGVeterinary Sciences2306-73812022-07-019737110.3390/vetsci9070371The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy FarmsAdin Shwimmer0Rama Falk1Tamar K. De-Levie2Michal Lasnoy3Katia Levytskyi4Amos Adler5National Service for Udder Health and Milk Quality, Israeli Dairy Board, Caesarea 38900, IsraelNational Service for Udder Health and Milk Quality, Israeli Dairy Board, Caesarea 38900, IsraelSackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, IsraelClinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Tel Aviv 64239, IsraelClinical Microbiology Laboratory, Tel Aviv Sourasky Medical Center, Tel Aviv 64239, IsraelSackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel<i>Staphylococcus aureus</i> is one of the most important pathogens causing intramammary infection (IMI) in dairy herds. The goals of this study were (1) to describe the prevalence of <i>S. aureus</i> in Israeli dairy farms; (2) to characterize the <i>spa</i>-based clonal structure of mastitis-related <i>S. aureus</i> isolates; (3) to analyze the transmission network of a large outbreak within a single farm and (4) to characterize the virulence factors of the outbreak strain. The prevalence and the molecular survey were performed on all Israeli IMI-related isolates, 9.2019–8.2020. Molecular methods included <i>spa</i>-typing for the survey and whole-genome sequencing (WGS) for the investigation of the farm ‘A’ outbreak. During the one-year survey, <i>S. aureus</i> was identified in 152 dairy farms, with a total of 440 positive samples. The <i>spa</i> t2873 was found in 284 isolates (64.5%) across 112 farms (73.6%). Other common types included t529 (n = 46), t9303 (n = 34) and the methicillin-resistant <i>S. aureus</i> t011 (n = 11). The highest number of cases (n = 25) was detected in Farm ‘A’, all of which were found to be <i>spa</i> t2873. Phylogenetic analysis confirmed that most transmission events occurred within the same milking group, and inter-group transmission was due to the transfer of cows between groups or due to consecutive milking order. The <i>spa</i> t2873 strain contained putative virulence genes, including various intracellular and collagen adhesion proteins. Our study revealed the dissemination of the t2873 strain to the majority of Israeli dairy farms. The possibility of inter-farm transmission should be monitored and prevented.https://www.mdpi.com/2306-7381/9/7/371<i>Staphylococcus aureus</i>bovine mastitistransmissionwhole-genome sequencing |
| spellingShingle | Adin Shwimmer Rama Falk Tamar K. De-Levie Michal Lasnoy Katia Levytskyi Amos Adler The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms Veterinary Sciences <i>Staphylococcus aureus</i> bovine mastitis transmission whole-genome sequencing |
| title | The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms |
| title_full | The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms |
| title_fullStr | The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms |
| title_full_unstemmed | The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms |
| title_short | The Dissemination of a Single <i>Staphylococcus</i><i>aureus</i> Strain, <i>Spa</i>-t2873, as the Predominant Cause of Bovine Mastitis in Israeli Dairy Farms |
| title_sort | dissemination of a single i staphylococcus i i aureus i strain i spa i t2873 as the predominant cause of bovine mastitis in israeli dairy farms |
| topic | <i>Staphylococcus aureus</i> bovine mastitis transmission whole-genome sequencing |
| url | https://www.mdpi.com/2306-7381/9/7/371 |
| work_keys_str_mv | AT adinshwimmer thedisseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT ramafalk thedisseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT tamarkdelevie thedisseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT michallasnoy thedisseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT katialevytskyi thedisseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT amosadler thedisseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT adinshwimmer disseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT ramafalk disseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT tamarkdelevie disseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT michallasnoy disseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT katialevytskyi disseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms AT amosadler disseminationofasingleistaphylococcusiiaureusistrainispait2873asthepredominantcauseofbovinemastitisinisraelidairyfarms |