Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation
Microsclerotia (MS) formation was successfully induced in Metarhizium rileyi (Ascomycetes: Hypocreales) in liquid culture. To optimise the process variables of liquid fermentation, we first used a two-level fraction design to confirm the variables, including inoculum density, initial pH, shaker spee...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Mycology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21501203.2017.1279688 |
| _version_ | 1818477946407485440 |
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| author | Zhangyong Song Yunlong Lin Fang Du Youping Yin Zhongkang Wang |
| author_facet | Zhangyong Song Yunlong Lin Fang Du Youping Yin Zhongkang Wang |
| author_sort | Zhangyong Song |
| collection | DOAJ |
| description | Microsclerotia (MS) formation was successfully induced in Metarhizium rileyi (Ascomycetes: Hypocreales) in liquid culture. To optimise the process variables of liquid fermentation, we first used a two-level fraction design to confirm the variables, including inoculum density, initial pH, shaker speed, and temperature, affecting M. rileyi MS production. Three variables were found to be important. Subsequently, a 23 full factorial central composite design (CCD) and response surface methodology were applied to ascertain the optimal level of each variable. A second-order polynomial was determined and shaker speed and inoculum density were found to be the primary variables affecting MS yields. Finally, we realised and optimised M. rileyi MS submerged fermentation based on previous findings. A maximum MS yields (3.84 × 104 MS/mL) were recorded in submerged fermentation at an initial pH of 5.5, growth temperature of 26°C, inoculum density of 10%, higher aeration rate (150 rpm in the initial 3 days and 200 rpm in the subsequent 3 days), and higher agitation rate of 800 L/h sterile air. |
| first_indexed | 2024-12-10T09:42:27Z |
| format | Article |
| id | doaj.art-a982ee724e9a408c9ac1cf3c2803ca5c |
| institution | Directory Open Access Journal |
| issn | 2150-1203 2150-1211 |
| language | English |
| last_indexed | 2024-12-10T09:42:27Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj.art-a982ee724e9a408c9ac1cf3c2803ca5c2022-12-22T01:53:58ZengTaylor & Francis GroupMycology2150-12032150-12112017-01-0181394710.1080/21501203.2017.12796881279688Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentationZhangyong Song0Yunlong Lin1Fang Du2Youping Yin3Zhongkang Wang4Chongqing UniversityChongqing UniversityChongqing UniversityChongqing UniversityChongqing UniversityMicrosclerotia (MS) formation was successfully induced in Metarhizium rileyi (Ascomycetes: Hypocreales) in liquid culture. To optimise the process variables of liquid fermentation, we first used a two-level fraction design to confirm the variables, including inoculum density, initial pH, shaker speed, and temperature, affecting M. rileyi MS production. Three variables were found to be important. Subsequently, a 23 full factorial central composite design (CCD) and response surface methodology were applied to ascertain the optimal level of each variable. A second-order polynomial was determined and shaker speed and inoculum density were found to be the primary variables affecting MS yields. Finally, we realised and optimised M. rileyi MS submerged fermentation based on previous findings. A maximum MS yields (3.84 × 104 MS/mL) were recorded in submerged fermentation at an initial pH of 5.5, growth temperature of 26°C, inoculum density of 10%, higher aeration rate (150 rpm in the initial 3 days and 200 rpm in the subsequent 3 days), and higher agitation rate of 800 L/h sterile air.http://dx.doi.org/10.1080/21501203.2017.1279688Metarhizium rileyimicrosclerotiasubmerged fermentationcentral composite designresponse surface methodologyaeration and agitation rate |
| spellingShingle | Zhangyong Song Yunlong Lin Fang Du Youping Yin Zhongkang Wang Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation Mycology Metarhizium rileyi microsclerotia submerged fermentation central composite design response surface methodology aeration and agitation rate |
| title | Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation |
| title_full | Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation |
| title_fullStr | Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation |
| title_full_unstemmed | Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation |
| title_short | Statistical optimisation of process variables and large-scale production of Metarhizium rileyi (Ascomycetes: Hypocreales) microsclerotia in submerged fermentation |
| title_sort | statistical optimisation of process variables and large scale production of metarhizium rileyi ascomycetes hypocreales microsclerotia in submerged fermentation |
| topic | Metarhizium rileyi microsclerotia submerged fermentation central composite design response surface methodology aeration and agitation rate |
| url | http://dx.doi.org/10.1080/21501203.2017.1279688 |
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