Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS
The effective production and usage of ginsenosides, given their distinct pharmacological effects, are receiving increasing amounts of attention. As the ginsenosides content differs in different parts of Panax ginseng, we wanted to assess and compare the ginsenosides content in the ginseng roots, lea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/12/2147 |
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| author | Jae Won Lee Bo-Ram Choi Young-Chang Kim Doo Jin Choi Young-Seob Lee Geum-Soog Kim Nam-In Baek Seung-Yu Kim Dae Young Lee |
| author_facet | Jae Won Lee Bo-Ram Choi Young-Chang Kim Doo Jin Choi Young-Seob Lee Geum-Soog Kim Nam-In Baek Seung-Yu Kim Dae Young Lee |
| author_sort | Jae Won Lee |
| collection | DOAJ |
| description | The effective production and usage of ginsenosides, given their distinct pharmacological effects, are receiving increasing amounts of attention. As the ginsenosides content differs in different parts of Panax ginseng, we wanted to assess and compare the ginsenosides content in the ginseng roots, leave, stems, and berries. To extract the ginsenosides, 70% (v/v) methanol was used. The optimal ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) method was used to profile various ginsenosides from the different parts of P. ginseng. The datasets were then subjected to multivariate analysis including principal component analysis (PCA) and hierarchical clustering analysis (HCA). A UPLC-QTOF/MS method with an in-house library was constructed to profile 58 ginsenosides. With this method, a total of 39 ginsenosides were successfully identified and quantified in the ginseng roots, leave, stem, and berries. PCA and HCA characterized the different ginsenosides compositions from the different parts. The quantitative ginsenoside contents were also characterized from each plant part. The results of this study indicate that the UPLC-QTOF/MS method can be an effective tool to characterize various ginsenosides from the different parts of P. ginseng. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T13:38:54Z |
| publishDate | 2017-12-01 |
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| series | Molecules |
| spelling | doaj.art-a98379ea06d04f349b884377e4c29db32022-12-21T17:44:56ZengMDPI AGMolecules1420-30492017-12-012212214710.3390/molecules22122147molecules22122147Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MSJae Won Lee0Bo-Ram Choi1Young-Chang Kim2Doo Jin Choi3Young-Seob Lee4Geum-Soog Kim5Nam-In Baek6Seung-Yu Kim7Dae Young Lee8Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaDepartment of Oriental Medicine Biotechnology, Kyung Hee University, Yongin 17104, KoreaProtected Horticulture Research Institute, National Institute of Horticultural and Herbal Science, RDA, Haman 52054, KoreaDepartment of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, KoreaThe effective production and usage of ginsenosides, given their distinct pharmacological effects, are receiving increasing amounts of attention. As the ginsenosides content differs in different parts of Panax ginseng, we wanted to assess and compare the ginsenosides content in the ginseng roots, leave, stems, and berries. To extract the ginsenosides, 70% (v/v) methanol was used. The optimal ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF/MS) method was used to profile various ginsenosides from the different parts of P. ginseng. The datasets were then subjected to multivariate analysis including principal component analysis (PCA) and hierarchical clustering analysis (HCA). A UPLC-QTOF/MS method with an in-house library was constructed to profile 58 ginsenosides. With this method, a total of 39 ginsenosides were successfully identified and quantified in the ginseng roots, leave, stem, and berries. PCA and HCA characterized the different ginsenosides compositions from the different parts. The quantitative ginsenoside contents were also characterized from each plant part. The results of this study indicate that the UPLC-QTOF/MS method can be an effective tool to characterize various ginsenosides from the different parts of P. ginseng.https://www.mdpi.com/1420-3049/22/12/2147Panax ginsengginsenosidesUPLC-QTOF/MS |
| spellingShingle | Jae Won Lee Bo-Ram Choi Young-Chang Kim Doo Jin Choi Young-Seob Lee Geum-Soog Kim Nam-In Baek Seung-Yu Kim Dae Young Lee Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS Molecules Panax ginseng ginsenosides UPLC-QTOF/MS |
| title | Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS |
| title_full | Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS |
| title_fullStr | Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS |
| title_full_unstemmed | Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS |
| title_short | Comprehensive Profiling and Quantification of Ginsenosides in the Root, Stem, Leaf, and Berry of Panax ginseng by UPLC-QTOF/MS |
| title_sort | comprehensive profiling and quantification of ginsenosides in the root stem leaf and berry of panax ginseng by uplc qtof ms |
| topic | Panax ginseng ginsenosides UPLC-QTOF/MS |
| url | https://www.mdpi.com/1420-3049/22/12/2147 |
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