Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis
Background The pro-inflammatory cytokine, tumor necrosis factor-alpha (TNF-α), is a pathogenic element for a number of disorders. Previous studies have reported that the -1031 T/C and -238 G/A polymorphisms in the promoter region of the TNF-α gene are important factors in reproductive-related disord...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2015-10-01
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| Series: | International Journal of Fertility and Sterility |
| Subjects: | |
| Online Access: | http://www.ijfs.ir/article_45321_6e6330b01fbf1a0a056cd3d689f3ad8f.pdf |
| _version_ | 1828476316536537088 |
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| author | Roshanak Abutorabi Azar Baradaran Fatemeh Sadat Mostafavi Yasaman Zarrin Farahnaz Mardanian |
| author_facet | Roshanak Abutorabi Azar Baradaran Fatemeh Sadat Mostafavi Yasaman Zarrin Farahnaz Mardanian |
| author_sort | Roshanak Abutorabi |
| collection | DOAJ |
| description | Background The pro-inflammatory cytokine, tumor necrosis factor-alpha (TNF-α), is a pathogenic element for a number of disorders. Previous studies have reported that the -1031 T/C and -238 G/A polymorphisms in the promoter region of the TNF-α gene are important factors in reproductive-related disorders. One of the most common gynecological diseases of women during the reproductive years is endometriosis. This study aims to assess an association between the -1031 T/C, -238 G/A and -308 G/A polymorphisms of the TNF-α gene promoter region to endometriosis. Materials and Methods In this case-control study, we enrolled 65 endometriosis patients and 65 matched healthy control women by simple sampling. Polymerase chain reaction (PCR) analysis was used to analyze -1031 T/C, -238 G/A and -308 G/A polymorphisms in the TNF-α gene promoter region. Statistical analysis was performed using the chi-square test. P values less than 0.05 were considered statistically significant. Results We found a strong association between the -1031 T/C polymorphism in the promoter region of the TNF-α gene with endometriosis (P=0.001). There were no significant associations between the -238 G/A (P=0.243) and -308 G/A (P=1) polymorphisms with endometriosis and again endometriosis stages have no association with these polymorphisms. Conclusion The -1031 T/C polymorphism and CC genotype can be used as a relevant marker to identify women at risk of developing endometriosis. |
| first_indexed | 2024-12-11T06:27:05Z |
| format | Article |
| id | doaj.art-a988df1d98584466adc026c0eccec533 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-11T06:27:05Z |
| publishDate | 2015-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-a988df1d98584466adc026c0eccec5332022-12-22T01:17:39ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782015-10-019332933710.22074/ijfs.2015.454845321Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in EndometriosisRoshanak Abutorabi0Azar Baradaran1Fatemeh Sadat Mostafavi2Yasaman Zarrin3Farahnaz Mardanian4Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of MedicalDepartment of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran4School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran5Department of Obstetrics and Gynecology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground The pro-inflammatory cytokine, tumor necrosis factor-alpha (TNF-α), is a pathogenic element for a number of disorders. Previous studies have reported that the -1031 T/C and -238 G/A polymorphisms in the promoter region of the TNF-α gene are important factors in reproductive-related disorders. One of the most common gynecological diseases of women during the reproductive years is endometriosis. This study aims to assess an association between the -1031 T/C, -238 G/A and -308 G/A polymorphisms of the TNF-α gene promoter region to endometriosis. Materials and Methods In this case-control study, we enrolled 65 endometriosis patients and 65 matched healthy control women by simple sampling. Polymerase chain reaction (PCR) analysis was used to analyze -1031 T/C, -238 G/A and -308 G/A polymorphisms in the TNF-α gene promoter region. Statistical analysis was performed using the chi-square test. P values less than 0.05 were considered statistically significant. Results We found a strong association between the -1031 T/C polymorphism in the promoter region of the TNF-α gene with endometriosis (P=0.001). There were no significant associations between the -238 G/A (P=0.243) and -308 G/A (P=1) polymorphisms with endometriosis and again endometriosis stages have no association with these polymorphisms. Conclusion The -1031 T/C polymorphism and CC genotype can be used as a relevant marker to identify women at risk of developing endometriosis.http://www.ijfs.ir/article_45321_6e6330b01fbf1a0a056cd3d689f3ad8f.pdfendometriosistumor necrosis factorpolymorphism |
| spellingShingle | Roshanak Abutorabi Azar Baradaran Fatemeh Sadat Mostafavi Yasaman Zarrin Farahnaz Mardanian Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis International Journal of Fertility and Sterility endometriosis tumor necrosis factor polymorphism |
| title | Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis |
| title_full | Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis |
| title_fullStr | Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis |
| title_full_unstemmed | Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis |
| title_short | Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis |
| title_sort | evaluation of tumor necrosis factor alpha polymorphism frequencies in endometriosis |
| topic | endometriosis tumor necrosis factor polymorphism |
| url | http://www.ijfs.ir/article_45321_6e6330b01fbf1a0a056cd3d689f3ad8f.pdf |
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