Isolation and Characterisation of Electrogenic Bacteria from Mud Samples
To develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation ca...
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/781 |
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| author | György Schneider Dorina Pásztor Péter Szabó László Kőrösi Nandyala Siva Kishan Penmetsa Appala Rama Krishna Raju Rajnish Kaur Calay |
| author_facet | György Schneider Dorina Pásztor Péter Szabó László Kőrösi Nandyala Siva Kishan Penmetsa Appala Rama Krishna Raju Rajnish Kaur Calay |
| author_sort | György Schneider |
| collection | DOAJ |
| description | To develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation capacities and macromolecule degradation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identifications have revealed that isolates represented 18 known and 4 unknown genuses. They all had the capacities to reduce the Reactive Black 5 stain in the agar medium, and 48 of them were positive in the wolfram nanorod reduction assay. The isolates formed biofilm to different extents on the surfaces of both adhesive and non-adhesive 96-well polystyrene plates and glass. Scanning electron microscopy images revealed the different adhesion potentials of isolates to the surface of carbon tissue fibres. Eight of them (15%) were able to form massive amounts of biofilm in three days at 23 °C. A total of 70% of the isolates produced proteases, while lipase and amylase production was lower, at 38% and 27% respectively. All of the macromolecule-degrading enzymes were produced by 11 isolates, and two isolates of them had the capacity to form a strong biofilm on the carbon tissue one of the most used anodic materials in MFC systems. This study discusses the potential of the isolates for future MFC development applications. |
| first_indexed | 2024-03-11T06:09:31Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T06:09:31Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a988e2eb28ce4653b29c1905b0a110cd2023-11-17T12:46:00ZengMDPI AGMicroorganisms2076-26072023-03-0111378110.3390/microorganisms11030781Isolation and Characterisation of Electrogenic Bacteria from Mud SamplesGyörgy Schneider0Dorina Pásztor1Péter Szabó2László Kőrösi3Nandyala Siva Kishan4Penmetsa Appala Rama Krishna Raju5Rajnish Kaur Calay6Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryDepartment of Geology and Meteorology, Faculty of Sciences, University of Pécs, Ifjúság Str. 6, H-7624 Pécs, HungaryResearch Institute for Viticulture and Oenology, University of Pécs, Pázmány P. u. 4, H-7634 Pécs, HungaryCentre for Research and Development, SRKR Engineering College, SRKR Marg, China Amiram, Bhimavaram 534204, IndiaDepartment of Civil Engineering, SRKR Engineering College, SRKR Marg, China Amiram, Bhimavaram 534024, IndiaInstitute for Building Energy and Materials Technology, Narvik Campus, UiT Norway’s Arctic University, 8514 Narvik, NorwayTo develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation capacities and macromolecule degradation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identifications have revealed that isolates represented 18 known and 4 unknown genuses. They all had the capacities to reduce the Reactive Black 5 stain in the agar medium, and 48 of them were positive in the wolfram nanorod reduction assay. The isolates formed biofilm to different extents on the surfaces of both adhesive and non-adhesive 96-well polystyrene plates and glass. Scanning electron microscopy images revealed the different adhesion potentials of isolates to the surface of carbon tissue fibres. Eight of them (15%) were able to form massive amounts of biofilm in three days at 23 °C. A total of 70% of the isolates produced proteases, while lipase and amylase production was lower, at 38% and 27% respectively. All of the macromolecule-degrading enzymes were produced by 11 isolates, and two isolates of them had the capacity to form a strong biofilm on the carbon tissue one of the most used anodic materials in MFC systems. This study discusses the potential of the isolates for future MFC development applications.https://www.mdpi.com/2076-2607/11/3/781electrogenic bacteriaidentificationmudbiofilmabiotic surfacecarbon tissue |
| spellingShingle | György Schneider Dorina Pásztor Péter Szabó László Kőrösi Nandyala Siva Kishan Penmetsa Appala Rama Krishna Raju Rajnish Kaur Calay Isolation and Characterisation of Electrogenic Bacteria from Mud Samples Microorganisms electrogenic bacteria identification mud biofilm abiotic surface carbon tissue |
| title | Isolation and Characterisation of Electrogenic Bacteria from Mud Samples |
| title_full | Isolation and Characterisation of Electrogenic Bacteria from Mud Samples |
| title_fullStr | Isolation and Characterisation of Electrogenic Bacteria from Mud Samples |
| title_full_unstemmed | Isolation and Characterisation of Electrogenic Bacteria from Mud Samples |
| title_short | Isolation and Characterisation of Electrogenic Bacteria from Mud Samples |
| title_sort | isolation and characterisation of electrogenic bacteria from mud samples |
| topic | electrogenic bacteria identification mud biofilm abiotic surface carbon tissue |
| url | https://www.mdpi.com/2076-2607/11/3/781 |
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