Comparative Genomics of Vibrio cholerae from Haiti, Asia, and Africa

Comparative Genomics of Vibrio cholerae from Haiti, Asia, and Africa

Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti in the fall of 2010. Pulsed-field gel electrophoresis (PFGE) analysis of clinical isolates from the Haiti outbreak and recent global travelers returning to the United States showed indistinguis...

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Bibliographic Details
Main Authors: Aleisha R. Reimer, Gary Van Domselaar, Steven Stroika, Matthew Walker, Heather Kent, Cheryl Tarr, Deborah Talkington, Lori A. Rowe, Melissa Olsen-Rasmussen, Michael Frace, Scott A. Sammons, Georges Anicet Dahourou, Jacques Boncy, Anthony M. Smith, Philip Mabon, Aaron Petkau, Morag Graham, Matthew W. Gilmour, Peter Gerner-Smidt
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2011-11-01
Series:Emerging Infectious Diseases
Subjects:
Vibrio cholerae
cholera
bacteria
genomics
whole-genome sequencing
public health
Online Access:https://wwwnc.cdc.gov/eid/article/17/11/11-0794_article
Description
Summary:Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti in the fall of 2010. Pulsed-field gel electrophoresis (PFGE) analysis of clinical isolates from the Haiti outbreak and recent global travelers returning to the United States showed indistinguishable PFGE fingerprints. To better explore the genetic ancestry of the Haiti outbreak strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 isolates obtained in Haiti or the Dominican Republic, 12 PFGE pattern-matched isolates linked to Asia or Africa, and 2 nonmatched outliers from the Western Hemisphere. Phylogenies for whole-genome sequences and core genome single-nucleotide polymorphisms showed that the Haiti outbreak strain is genetically related to strains originating in India and Cameroon. However, because no identical genetic match was found among sequenced contemporary isolates, a definitive genetic origin for the outbreak in Haiti remains speculative.
ISSN:1080-6040
1080-6059

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