Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study
The aim of the study was to evaluate the occurrence of Arcobacter spp. in food samples collected from Sicilia region. A total of 91 food products of animal origin (41 meat, 17 fresh milk, 18 shellfish) and 15 samples of fresh vegetables, were examined by cultural method and confirmed by biochemical...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2018-07-01
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| Series: | Italian Journal of Food Safety |
| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/index.php/ijfs/article/view/7171 |
| _version_ | 1811322242662924288 |
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| author | Anna Maria Di Noto Sonia Sciortino Cinzia Cardamone Cosimo Ciravolo Concetta Napoli Vincenzina Alio Pietro Arculeo Giuseppa Oliveri Antonella Costa |
| author_facet | Anna Maria Di Noto Sonia Sciortino Cinzia Cardamone Cosimo Ciravolo Concetta Napoli Vincenzina Alio Pietro Arculeo Giuseppa Oliveri Antonella Costa |
| author_sort | Anna Maria Di Noto |
| collection | DOAJ |
| description | The aim of the study was to evaluate the occurrence of Arcobacter spp. in food samples collected from Sicilia region. A total of 91 food products of animal origin (41 meat, 17 fresh milk, 18 shellfish) and 15 samples of fresh vegetables, were examined by cultural method and confirmed by biochemical analysis and PCR methods. The detection of Arcobacter spp. was performed, after selective enrichment, on two selective agar plates: Arcobacter agar and mCCD (modified charcoal cefoperazone deoxycholate) agar supplemented with CAT (Cefoperazone, Amphotericin B and Teicoplanin). Arcobacter species were isolated using the membrane filtration technique. In 13 (14.3%) out of the 91 tested samples, the presence of Arcobacter spp. was found: the isolates were confirmed by multiplex PCR and identified as belonging to the species A. butzleri and A. cryaerophilus. The highest prevalence rate was observed in chicken meat (8.8%) followed by shellfish (3.3%). Negative results have been obtained for raw milks and vegetables samples. The preliminary study highlights the importance of this emerging pathogen and the need for further studies on its prevalence and distribution in different types of food for human consumption. |
| first_indexed | 2024-04-13T13:32:54Z |
| format | Article |
| id | doaj.art-a98c94b03af7411e9fc43d116ad036bd |
| institution | Directory Open Access Journal |
| issn | 2239-7132 |
| language | English |
| last_indexed | 2024-04-13T13:32:54Z |
| publishDate | 2018-07-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Food Safety |
| spelling | doaj.art-a98c94b03af7411e9fc43d116ad036bd2022-12-22T02:44:55ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322018-07-017210.4081/ijfs.2018.71715758Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary studyAnna Maria Di Noto0Sonia Sciortino1Cinzia Cardamone2Cosimo Ciravolo3Concetta Napoli4Vincenzina Alio5Pietro Arculeo6Giuseppa Oliveri7Antonella Costa8Food Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoFood Microbiology Section, Experimental Zooprophylactic Institute of Sicily, PalermoThe aim of the study was to evaluate the occurrence of Arcobacter spp. in food samples collected from Sicilia region. A total of 91 food products of animal origin (41 meat, 17 fresh milk, 18 shellfish) and 15 samples of fresh vegetables, were examined by cultural method and confirmed by biochemical analysis and PCR methods. The detection of Arcobacter spp. was performed, after selective enrichment, on two selective agar plates: Arcobacter agar and mCCD (modified charcoal cefoperazone deoxycholate) agar supplemented with CAT (Cefoperazone, Amphotericin B and Teicoplanin). Arcobacter species were isolated using the membrane filtration technique. In 13 (14.3%) out of the 91 tested samples, the presence of Arcobacter spp. was found: the isolates were confirmed by multiplex PCR and identified as belonging to the species A. butzleri and A. cryaerophilus. The highest prevalence rate was observed in chicken meat (8.8%) followed by shellfish (3.3%). Negative results have been obtained for raw milks and vegetables samples. The preliminary study highlights the importance of this emerging pathogen and the need for further studies on its prevalence and distribution in different types of food for human consumption.https://www.pagepressjournals.org/index.php/ijfs/article/view/7171Arcobacter spp., Foodborne pathogens, Multiplex PCR. |
| spellingShingle | Anna Maria Di Noto Sonia Sciortino Cinzia Cardamone Cosimo Ciravolo Concetta Napoli Vincenzina Alio Pietro Arculeo Giuseppa Oliveri Antonella Costa Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study Italian Journal of Food Safety Arcobacter spp., Foodborne pathogens, Multiplex PCR. |
| title | Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study |
| title_full | Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study |
| title_fullStr | Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study |
| title_full_unstemmed | Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study |
| title_short | Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study |
| title_sort | detection of arcobacter spp in food products collected from sicilia region a preliminary study |
| topic | Arcobacter spp., Foodborne pathogens, Multiplex PCR. |
| url | https://www.pagepressjournals.org/index.php/ijfs/article/view/7171 |
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