Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis
Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sug...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-07-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00245/full |
| _version_ | 1818425020370649088 |
|---|---|
| author | Timothy J Heisel Timothy J Heisel Chun Yao eLi Katia M Grey Susan I Gibson |
| author_facet | Timothy J Heisel Timothy J Heisel Chun Yao eLi Katia M Grey Susan I Gibson |
| author_sort | Timothy J Heisel |
| collection | DOAJ |
| description | Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Towards this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism. |
| first_indexed | 2024-12-14T14:07:18Z |
| format | Article |
| id | doaj.art-a98fa792b12b4e58ad0bb90818f9c32d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T14:07:18Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a98fa792b12b4e58ad0bb90818f9c32d2022-12-21T22:58:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2013-07-01410.3389/fpls.2013.0024551448Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in ArabidopsisTimothy J Heisel0Timothy J Heisel1Chun Yao eLi2Katia M Grey3Susan I Gibson4University of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaUniversity of MinnesotaNutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Towards this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00245/fullArabidopsisFertilityChromatin remodelingchromatin modificationSugar SignalingSnRK1 |
| spellingShingle | Timothy J Heisel Timothy J Heisel Chun Yao eLi Katia M Grey Susan I Gibson Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis Frontiers in Plant Science Arabidopsis Fertility Chromatin remodeling chromatin modification Sugar Signaling SnRK1 |
| title | Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis |
| title_full | Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis |
| title_fullStr | Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis |
| title_full_unstemmed | Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis |
| title_short | Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis |
| title_sort | mutations in histone acetyltransferase1 affect sugar response and gene expression in arabidopsis |
| topic | Arabidopsis Fertility Chromatin remodeling chromatin modification Sugar Signaling SnRK1 |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00245/full |
| work_keys_str_mv | AT timothyjheisel mutationsinhistoneacetyltransferase1affectsugarresponseandgeneexpressioninarabidopsis AT timothyjheisel mutationsinhistoneacetyltransferase1affectsugarresponseandgeneexpressioninarabidopsis AT chunyaoeli mutationsinhistoneacetyltransferase1affectsugarresponseandgeneexpressioninarabidopsis AT katiamgrey mutationsinhistoneacetyltransferase1affectsugarresponseandgeneexpressioninarabidopsis AT susanigibson mutationsinhistoneacetyltransferase1affectsugarresponseandgeneexpressioninarabidopsis |