Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i>
<i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. has been used as an herbal remedy and often involves using the whole plant or roots. However, the bioactivities of different parts of the plant have been far less explored. This study aimed to evaluate the antioxidative...
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| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/19/6923 |
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| author | Pei-Ling Yen Ting-An Lin Wei Lin Chuah Chih-Yi Chang Yen-Hsueh Tseng Chia-Yin Huang Jeng-Chuann Yang Fu-Lan Hsu Vivian Hsiu-Chuan Liao |
| author_facet | Pei-Ling Yen Ting-An Lin Wei Lin Chuah Chih-Yi Chang Yen-Hsueh Tseng Chia-Yin Huang Jeng-Chuann Yang Fu-Lan Hsu Vivian Hsiu-Chuan Liao |
| author_sort | Pei-Ling Yen |
| collection | DOAJ |
| description | <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. has been used as an herbal remedy and often involves using the whole plant or roots. However, the bioactivities of different parts of the plant have been far less explored. This study aimed to evaluate the antioxidative ability of methanol extracts from the flowers, leaves, stems, and roots of the <i>Cirsium</i> plant and their possible active components against juglone-induced oxidative stress in the nematode <i>Caenorhabditis elegans</i>. The results showed that the highest dry weight (12.3 g per plant) was observed in leaves, which was followed by stems (8.0 g). The methanol extract yields from the flowers, leaves, and roots were all similar (13.0–13.8%), while the yield from stems was the lowest (8.6%). The analysis of the silymarin contents in the extracts indicated that the flowers, leaves, stems, and roots contained silychristin and taxifolin; however, silydianin was only found in the leaves, stems, and roots. The flower, leaf, and stem extracts, at a concentration of 10 mg/L, significantly reduced juglone-induced oxidative stress in <i>C. elegans</i>, which was potentially due to the presence of silychristin and taxifolin. Overall, <i>C. japonicum</i> DC. var. <i>australe</i> Kitam. contains a significant amount of silymarin and exhibits in vivo antioxidative activity, suggesting that the prospects for the plant in terms of health supplements or as a source of silymarin are promising. |
| first_indexed | 2024-03-10T21:38:58Z |
| format | Article |
| id | doaj.art-a98ff8bcdd424a19ac0a0d323d911427 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:38:58Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a98ff8bcdd424a19ac0a0d323d9114272023-11-19T14:47:25ZengMDPI AGMolecules1420-30492023-10-012819692310.3390/molecules28196923Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i>Pei-Ling Yen0Ting-An Lin1Wei Lin Chuah2Chih-Yi Chang3Yen-Hsueh Tseng4Chia-Yin Huang5Jeng-Chuann Yang6Fu-Lan Hsu7Vivian Hsiu-Chuan Liao8Department of Bioenvironmental Systems Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, TaiwanDepartment of Bioenvironmental Systems Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, TaiwanDepartment of Bioenvironmental Systems Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, TaiwanDepartment of Forestry, National Chung Hsing University, No. 145, Xingda Rd., Taichung 402, TaiwanTaiwan Forestry Research Institute, No. 53, Nanhai Rd., Taipei 100, TaiwanTaiwan Forestry Research Institute, No. 53, Nanhai Rd., Taipei 100, TaiwanTaiwan Forestry Research Institute, No. 53, Nanhai Rd., Taipei 100, TaiwanTaiwan Forestry Research Institute, No. 53, Nanhai Rd., Taipei 100, TaiwanDepartment of Bioenvironmental Systems Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan<i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. has been used as an herbal remedy and often involves using the whole plant or roots. However, the bioactivities of different parts of the plant have been far less explored. This study aimed to evaluate the antioxidative ability of methanol extracts from the flowers, leaves, stems, and roots of the <i>Cirsium</i> plant and their possible active components against juglone-induced oxidative stress in the nematode <i>Caenorhabditis elegans</i>. The results showed that the highest dry weight (12.3 g per plant) was observed in leaves, which was followed by stems (8.0 g). The methanol extract yields from the flowers, leaves, and roots were all similar (13.0–13.8%), while the yield from stems was the lowest (8.6%). The analysis of the silymarin contents in the extracts indicated that the flowers, leaves, stems, and roots contained silychristin and taxifolin; however, silydianin was only found in the leaves, stems, and roots. The flower, leaf, and stem extracts, at a concentration of 10 mg/L, significantly reduced juglone-induced oxidative stress in <i>C. elegans</i>, which was potentially due to the presence of silychristin and taxifolin. Overall, <i>C. japonicum</i> DC. var. <i>australe</i> Kitam. contains a significant amount of silymarin and exhibits in vivo antioxidative activity, suggesting that the prospects for the plant in terms of health supplements or as a source of silymarin are promising.https://www.mdpi.com/1420-3049/28/19/6923antioxidative activity<i>Cirsium</i><i>Caenorhabditis elegans</i>oxidative stresssilymarin |
| spellingShingle | Pei-Ling Yen Ting-An Lin Wei Lin Chuah Chih-Yi Chang Yen-Hsueh Tseng Chia-Yin Huang Jeng-Chuann Yang Fu-Lan Hsu Vivian Hsiu-Chuan Liao Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i> Molecules antioxidative activity <i>Cirsium</i> <i>Caenorhabditis elegans</i> oxidative stress silymarin |
| title | Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i> |
| title_full | Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i> |
| title_fullStr | Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i> |
| title_short | Methanol Extracts from <i>Cirsium japonicum</i> DC. var. <i>australe</i> Kitam. and Their Active Components Reduce Intracellular Oxidative Stress in <i>Caenorhabditis elegans</i> |
| title_sort | methanol extracts from i cirsium japonicum i dc var i australe i kitam and their active components reduce intracellular oxidative stress in i caenorhabditis elegans i |
| topic | antioxidative activity <i>Cirsium</i> <i>Caenorhabditis elegans</i> oxidative stress silymarin |
| url | https://www.mdpi.com/1420-3049/28/19/6923 |
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