Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in Rats
Osteoarthritis (OA) represents the highest degenerative disorder. Because cartilage erosion is a common pathological alteration in OA, targeting some key metalloproteinases such as MMP-3, ADAMTS-5 besides their inhibitor TIMP-3 by natural products, could be an effective strategy to protect against o...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/16/2/260 |
| _version_ | 1797618832505831424 |
|---|---|
| author | Nema S. Shaban Abeer M. Radi Mohamed A. Abdelgawad Mohammed M. Ghoneim Rasha Hamed Al-Serwi Randa M. Hassan Eman T. Mohammed Rania A. Radi Fatma M. Halfaya |
| author_facet | Nema S. Shaban Abeer M. Radi Mohamed A. Abdelgawad Mohammed M. Ghoneim Rasha Hamed Al-Serwi Randa M. Hassan Eman T. Mohammed Rania A. Radi Fatma M. Halfaya |
| author_sort | Nema S. Shaban |
| collection | DOAJ |
| description | Osteoarthritis (OA) represents the highest degenerative disorder. Because cartilage erosion is a common pathological alteration in OA, targeting some key metalloproteinases such as MMP-3, ADAMTS-5 besides their inhibitor TIMP-3 by natural products, could be an effective strategy to protect against osteoarthritis. Forty female Wister rats were categorized into five equal groups. Control, osteoarthritic (OA) (monosodium iodoacetate (MIA) 2 mg/50 µL saline, single intra-articular injection), OA+ indomethacin (2 mg/kg/daily/orally), OA+ nano-naringenin (25 mg/kg/daily/orally), and OA+ <i>Amphora coffeaeformis</i> (772 mg/kg/daily/orally). Treatments were initiated on the 8th day after osteoarthritis induction and continued for 28 days thereafter. Finally, blood and knee joint samples were collected from all rats for biochemical and histopathological evaluations. The current study showed that MIA induced oxidative stress, which resulted in changes in the inflammatory joint markers associated with increased right knee diameter and higher clinical scores for lameness. <i>Amphora coffeaeformis</i> followed by nano-naringenin exhibited a potential anti-arthritic activity by reducing the concentrations of serum MMP-3, ADAMTS-5, and joint MDA and increasing the levels of serum TIMP-3 and joint GSH, similar to indomethacin. The histopathological results confirmed these outcomes. In conclusion, <i>Amphora coffeaeformis</i> and nano-naringenin can be considered as natural therapeutic agents for osteoarthritis owing to their antioxidant and anti-inflammatory activities. |
| first_indexed | 2024-03-11T08:18:10Z |
| format | Article |
| id | doaj.art-a99017631f2e46fd8ec5d3c05db9e5ca |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-11T08:18:10Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-a99017631f2e46fd8ec5d3c05db9e5ca2023-11-16T22:37:12ZengMDPI AGPharmaceuticals1424-82472023-02-0116226010.3390/ph16020260Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in RatsNema S. Shaban0Abeer M. Radi1Mohamed A. Abdelgawad2Mohammed M. Ghoneim3Rasha Hamed Al-Serwi4Randa M. Hassan5Eman T. Mohammed6Rania A. Radi7Fatma M. Halfaya8Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Aljouf 72341, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Ad Diriyah 13713, Saudi ArabiaDepartment of Basic Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, EgyptOsteoarthritis (OA) represents the highest degenerative disorder. Because cartilage erosion is a common pathological alteration in OA, targeting some key metalloproteinases such as MMP-3, ADAMTS-5 besides their inhibitor TIMP-3 by natural products, could be an effective strategy to protect against osteoarthritis. Forty female Wister rats were categorized into five equal groups. Control, osteoarthritic (OA) (monosodium iodoacetate (MIA) 2 mg/50 µL saline, single intra-articular injection), OA+ indomethacin (2 mg/kg/daily/orally), OA+ nano-naringenin (25 mg/kg/daily/orally), and OA+ <i>Amphora coffeaeformis</i> (772 mg/kg/daily/orally). Treatments were initiated on the 8th day after osteoarthritis induction and continued for 28 days thereafter. Finally, blood and knee joint samples were collected from all rats for biochemical and histopathological evaluations. The current study showed that MIA induced oxidative stress, which resulted in changes in the inflammatory joint markers associated with increased right knee diameter and higher clinical scores for lameness. <i>Amphora coffeaeformis</i> followed by nano-naringenin exhibited a potential anti-arthritic activity by reducing the concentrations of serum MMP-3, ADAMTS-5, and joint MDA and increasing the levels of serum TIMP-3 and joint GSH, similar to indomethacin. The histopathological results confirmed these outcomes. In conclusion, <i>Amphora coffeaeformis</i> and nano-naringenin can be considered as natural therapeutic agents for osteoarthritis owing to their antioxidant and anti-inflammatory activities.https://www.mdpi.com/1424-8247/16/2/260<i>Amphora coffeaeformis</i>nano-naringeninmetalloproteinasesrat MIA-osteoarthritis model |
| spellingShingle | Nema S. Shaban Abeer M. Radi Mohamed A. Abdelgawad Mohammed M. Ghoneim Rasha Hamed Al-Serwi Randa M. Hassan Eman T. Mohammed Rania A. Radi Fatma M. Halfaya Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in Rats Pharmaceuticals <i>Amphora coffeaeformis</i> nano-naringenin metalloproteinases rat MIA-osteoarthritis model |
| title | Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in Rats |
| title_full | Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in Rats |
| title_fullStr | Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in Rats |
| title_full_unstemmed | Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in Rats |
| title_short | Targeting Some Key Metalloproteinases by Nano-Naringenin and <i>Amphora coffeaeformis</i> as a Novel Strategy for Treatment of Osteoarthritis in Rats |
| title_sort | targeting some key metalloproteinases by nano naringenin and i amphora coffeaeformis i as a novel strategy for treatment of osteoarthritis in rats |
| topic | <i>Amphora coffeaeformis</i> nano-naringenin metalloproteinases rat MIA-osteoarthritis model |
| url | https://www.mdpi.com/1424-8247/16/2/260 |
| work_keys_str_mv | AT nemasshaban targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT abeermradi targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT mohamedaabdelgawad targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT mohammedmghoneim targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT rashahamedalserwi targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT randamhassan targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT emantmohammed targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT raniaaradi targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats AT fatmamhalfaya targetingsomekeymetalloproteinasesbynanonaringeninandiamphoracoffeaeformisiasanovelstrategyfortreatmentofosteoarthritisinrats |