Seroepidemiology of toxoplasmosis among people having close contact with animals
A cross-sectional study was conducted to determine the seroepidemiology of Toxoplasma gondii infection among individuals who have close contact with animals. The association between plausible risk factors and the seropositivity for Toxoplasma was examined. A total of 312 blood samples were collected...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00143/full |
| _version_ | 1819116249070698496 |
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| author | Guo-Jie eBrandon-Mong Nurul Asma Anati BT Che Mat Seri Reuben eSharma Hemah eAndiappan Tian-Chye eTan Yvonne Ai-Lian Lim Veeranoot eNISSAPATORN |
| author_facet | Guo-Jie eBrandon-Mong Nurul Asma Anati BT Che Mat Seri Reuben eSharma Hemah eAndiappan Tian-Chye eTan Yvonne Ai-Lian Lim Veeranoot eNISSAPATORN |
| author_sort | Guo-Jie eBrandon-Mong |
| collection | DOAJ |
| description | A cross-sectional study was conducted to determine the seroepidemiology of Toxoplasma gondii infection among individuals who have close contact with animals. The association between plausible risk factors and the seropositivity for Toxoplasma was examined. A total of 312 blood samples were collected form veterinarians(38), veterinary technicians(45), veterinary students(194) and pet owners(35) from several veterinary hospitals and clinics in the area of Klang Valley, Malaysia. About 4cc of blood samples drawn from agreed participants were processed for measurement of anti-Toxoplasma IgG and IgM antibodies as well as avidity test of Toxoplasma IgG by ELISA.Meanwhile, the demographic profiles and possible risk factors of these participants were also recorded in the standardized data collection sheets.Overall seroprevalence of toxoplasmosis was observed in 62 (19.9%)participants where 7(18.4%) veterinarians, 15(33.3%)veterinary technicians, 29(14.9%)veterinary students and 11(31.4%) pet owners were infected. Out of 19.9% positive samples for toxoplasmosis, 18.3% of these samples were positive for IgG antibody, 1.0% was positive for IgM antibody and 0.6% were positive for both IgG and IgM antibodies.Positive samples for IgM antibody and for both IgG and IgM antibodies were further analysed by IgG avidity test. Out of 5 samples tested for avidity test, 4 samples showed a high avidity results which means infection occurred in the past whereas only 1 sample showed a recently acquired infection. Univariate analysis showed that age group, gender,study population, gardening, task performance and working duration were significantly associated with seropositivity of toxoplasmosis. Further analysis by multivariate analysis using logistic regression showed that age group more than or equal to 30 years old (OR=0.34, 95% CI=0.18-0.63, p=0.001)and working or study duration more than 10 years in handling with animals(OR=5.07, 95% CI=1.80-14.24, p=0.002)were identified as significant |
| first_indexed | 2024-12-22T05:14:05Z |
| format | Article |
| id | doaj.art-a99248aefc094638965a01c997249c93 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T05:14:05Z |
| publishDate | 2015-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-a99248aefc094638965a01c997249c932022-12-21T18:37:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242015-04-01610.3389/fimmu.2015.00143124680Seroepidemiology of toxoplasmosis among people having close contact with animalsGuo-Jie eBrandon-Mong0Nurul Asma Anati BT Che Mat Seri1Reuben eSharma2Hemah eAndiappan3Tian-Chye eTan4Yvonne Ai-Lian Lim5Veeranoot eNISSAPATORN6University of MalayaUniversity of MalayaUniversiti Putra MalaysiaUniversity of MalayaUniversity of MalayaUniversity of MalayaUniversity of MalayaA cross-sectional study was conducted to determine the seroepidemiology of Toxoplasma gondii infection among individuals who have close contact with animals. The association between plausible risk factors and the seropositivity for Toxoplasma was examined. A total of 312 blood samples were collected form veterinarians(38), veterinary technicians(45), veterinary students(194) and pet owners(35) from several veterinary hospitals and clinics in the area of Klang Valley, Malaysia. About 4cc of blood samples drawn from agreed participants were processed for measurement of anti-Toxoplasma IgG and IgM antibodies as well as avidity test of Toxoplasma IgG by ELISA.Meanwhile, the demographic profiles and possible risk factors of these participants were also recorded in the standardized data collection sheets.Overall seroprevalence of toxoplasmosis was observed in 62 (19.9%)participants where 7(18.4%) veterinarians, 15(33.3%)veterinary technicians, 29(14.9%)veterinary students and 11(31.4%) pet owners were infected. Out of 19.9% positive samples for toxoplasmosis, 18.3% of these samples were positive for IgG antibody, 1.0% was positive for IgM antibody and 0.6% were positive for both IgG and IgM antibodies.Positive samples for IgM antibody and for both IgG and IgM antibodies were further analysed by IgG avidity test. Out of 5 samples tested for avidity test, 4 samples showed a high avidity results which means infection occurred in the past whereas only 1 sample showed a recently acquired infection. Univariate analysis showed that age group, gender,study population, gardening, task performance and working duration were significantly associated with seropositivity of toxoplasmosis. Further analysis by multivariate analysis using logistic regression showed that age group more than or equal to 30 years old (OR=0.34, 95% CI=0.18-0.63, p=0.001)and working or study duration more than 10 years in handling with animals(OR=5.07, 95% CI=1.80-14.24, p=0.002)were identified as significanthttp://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00143/fullPrevalenceprevalence.Toxoplasma gondiianti-Toxoplasma antibodiesrisk factors.IgG avidity |
| spellingShingle | Guo-Jie eBrandon-Mong Nurul Asma Anati BT Che Mat Seri Reuben eSharma Hemah eAndiappan Tian-Chye eTan Yvonne Ai-Lian Lim Veeranoot eNISSAPATORN Seroepidemiology of toxoplasmosis among people having close contact with animals Frontiers in Immunology Prevalence prevalence. Toxoplasma gondii anti-Toxoplasma antibodies risk factors. IgG avidity |
| title | Seroepidemiology of toxoplasmosis among people having close contact with animals |
| title_full | Seroepidemiology of toxoplasmosis among people having close contact with animals |
| title_fullStr | Seroepidemiology of toxoplasmosis among people having close contact with animals |
| title_full_unstemmed | Seroepidemiology of toxoplasmosis among people having close contact with animals |
| title_short | Seroepidemiology of toxoplasmosis among people having close contact with animals |
| title_sort | seroepidemiology of toxoplasmosis among people having close contact with animals |
| topic | Prevalence prevalence. Toxoplasma gondii anti-Toxoplasma antibodies risk factors. IgG avidity |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2015.00143/full |
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